“…The LT-PO-DAB procedures have recently been employed for the histochemical detection of saccharide residues of carbohydrates in light microscopy (Streit et al , 1985;Hosaka et al , 1985;Capaldi et al , 1985;Dolapchieva et al , 1986;Kashio et al , 1991;Martinez-Menarguez et al , 1992;Gheri et al , 1993), even though the final reaction products obtained suffer at times from their diffusion (Fujimori et al , 1988). The AB methods are known to be precise histochemical techniques for the demonstration of acidic groupings of carbohydrates and have long been used widely in light microscopy (Spicer et al , 1967;Pearse, 1985;Yamada, 1987), since the dye was first introduced by Steedman (1950) into light microscopic histochemistry of mucins.…”