Binding of lactoferrin to the surface of low-density lipoproteins modified by myeloperoxidase prevents intracellular cholesterol accumulation by human blood monocytes

Abstract

Myeloperoxidase (MPO) is unique heme-containing peroxidase which can catalyze formation of hypochlorous acid (HOCl). Strong interaction of MPO with low-density lipoproteins (LDL) promotes proatherogenic modification of LDL by HOCl. The so-called MPO-modified LDL (Mox-LDL) accumulates in macrophages with formation of foam cells, which is the pathognomic symptom of atherosclerosis. A promising approach to prophylaxis and therapy of atherosclerosis is searching for remedies preventing modification or accumulation of LDL in macrophages. Lactoferrin (LF) has several application points in obesity pathogenesis. We aimed to study LF binding to Mox-LDL and their accumulation in monocytes transformed into macrophages. Using surface plasmon resonance and ELISA techniques we observed no LF interaction with intact LDL, while Mox-LDL strongly interacted with LF. Affinity of Mox-LDL to LF increased with the degree of oxidative modification of LDL. Moreover, an excess of MPO did not prevent interaction of Mox-LDL with LF. LF inhibits accumulation of cholesterol in macrophages exposed to Mox-LDL. The results obtained reinforce the notion of LF potency as a remedy against atherosclerosis.

Additive Production of a Material Based on an Acrylic Polymer with a Nanoscale Layer of Zno Nanorods Deposited Using a Direct Current Magnetron Discharge: Morphology, Photoconversion Properties, and Biosafety

“…It is known that generation of reactive oxygen species (ROS) is observed on the surface of zinc oxide coatings in in-vitro systems [ 52 ]. It is known that an increase in the intracellular concentration of ROS often leads to the development of oxidative stress [ 53 ] associated with lipid peroxidation [ 54 ], oxidative modification of proteins [ 55 ], and nucleic acids [ 56 ]. Damage to biological molecules is associated with processes, such as mutagenesis, carcinogenesis, teratogenesis, aging, etc.…”

Inflammatory markers and risk of cardiovascular mortality in relation to diabetes status in the HUNT study

“…Studies show that increasing concentrations of neopterin can predict cardiac events in patients with chronic stable angina 12 and acute coronary syndromes 13 , as well as fatal cardiac events in patients with diabetes 4 . Lactoferrin is a glycoprotein released from secondary granules of activated neutrophils, thought to have anti-atherogenic properties, such as inhibiting expression of endothelial adhesion molecules 14 and cholesterol accumulation in macrophages 15 . Increasing lactoferrin concentrations correlate with the degree of coronary artery stenosis in patients undergoing angiography 16 and are suggested to predict fatal cardiac events in patients with diabetes 3 .…”

Znaczenie i wykorzystanie białkowych składników serwatki

“…Białka serwatkowe mają też coraz większe znaczenie w medycynie, czy to bezpośrednio, jako substancje o działaniu terapeutycznym (Amirani i in., 2020;, 2013Ng i in., 2015;Sousa i in., 2012), czy też pośrednio, jako substancje służące do enkapsulacji leków oraz innych bioaktywnych substancji, co wynika z ich zdolności tworzenia nanokompozytów (Livney, 2010;Varlamova i Zaripow, 2020). Jak dowiedziono, białka te wykazują działanie przeciwzapalne, wpływają korzystnie na funkcjonowanie układu odpornościowego, regulację ciśnienia tętniczego oraz poziomu glukozy i cholesterolu we krwi, jak również mają właściwości antynowotworowe (Cicero i in., 2017;Krissansen i in., 2007;Sousa i in., 2012;Teixeira i in., 2019;Vasilyev i in. 2021;Yang i in.…”