Binding of human serum albumin to silica particles by means of polymers: a liquid chromatographic study of the selectivity of resulting chiral stationary phases

Millot, Marie‐Claude; Taleb, Nabila L; Sébille, Bernard

doi:10.1016/s0378-4347(01)00495-9

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…CSPs obtained by immobilization of human serum albumin on various polymer-coated silicas were investigated for the enantioseparation of racemic mixtures of tryptophan, oxazepam, warfarin, and NBP (153). An immobilized human serum albumin CSP was used in the study of the stereoselective binding of 2-(4-biphenylyl)-3-substituted 3-hydroxypropionic acids (154).…”

Section: Liquid Chromatographymentioning

confidence: 99%

Chiral Separations

2004

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Gas Chromatography (GC). The separation of enantiomers by GC on CSPs was extensively reviewed by Schurig (12), with discussion of the method development, applications, and ancillary techniques of chiral separations using GC. CSPs with amino acid derivatives, terpene-derived metal coordination compounds, and modified cyclodextrins were included. Schurig also comprehensively reviewed the practice and theory of enantioselective complexation GC on optically active metal(II) bis[3-perfluoroacyl)-(1R)-camphorate] selectors (13). Applications extend to chiral analysis in asymmetric synthesis, enzymic reactions, pheromone, and flavor chemistry. The elucidation of thermodynamic parameters of enantioselectivity and the investigation of the enantiomerization of configurationally labile enantiomers was also discussed.Thin-Layer Chromatography (TLC). The development of stationary phases for TLC in the last 10 years was compiled by Gocan ( 14), and included chiral separation and recent advances in chiral stationary phases. The CSPs discussed included nonpolar bonded stationary phases impregnated with transitional metal ions, cellulose, modified cellulose, chitin, chitosan, and their derivatives. Cyclodextrin and macrocyclic antibiotics were reported to have very good results for enantioseparation by TLC, with molecular imprinting polymers also finding use as CSPs in TLC.Capillary Electrochromatography. The applications of CEC were reviewed by Remcho et al. (15). Chiral separations with cyclodextrins and their derivatives, biomolecules, molecularly imprinted polymers, "brush"-type phases, ion exchangers, antibiotics, polysaccharide derivatives, and other CSPs were included in this review. A summary of recent progress in open-tubular CEC for chiral and achiral separations included stationary-phase preparation ( 16), with the major developments, potential applications, technical difficulties, and advantages of each wall coating discussed.Multiple Technique Reviews. The use of cyclodextrins in chiral chromatography was compiled by Juvancz and Szejtli (17). The role of cyclodextrins in methods using capillary columns such as GC, supercritical fluid chromatography (SFC), and CE was detailed, as well as their use in other forms of chromatography such as HPLC and TLC. The mechanism of chiral recognition using cyclodextrins was also discussed. Williams and Wainer (18) reviewed the role of chiral chromatography in therapeutic drug monitoring and in clinical and forensic toxicology. Enantioselective GC and HPLC were used as tools to unravel complex phenomena associated with drug transport and metabolism.

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Section: Liquid Chromatographymentioning

confidence: 99%

Chiral Separations

2004

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“…The earliest work in the area of human serum immobilized chromatography was carried out by Wainer and his group [34][35][36][37]. Henceforth, different strategies for the development of columns immobilized with HSA have been reported [38][39][40][41][42][43][44]. In one method, the epoxy groups of this copolymer (glycidyl methacrylate and ethylene dimethacrylate) were used directly for the immobilization of HSA through its amine residues (i.e., the epoxy method); in other approaches, these epoxy groups were converted to diols for later use in the carbonyldiimidazole, disuccinimidyl carbonate, and Schiff base methods [38].…”

Section: Introductionmentioning

confidence: 99%

Measurement of drug–protein binding by immobilized human serum albumin-HPLC and comparison with ultrafiltration

Singh¹,

Mehta²

2006

Journal of Chromatography B

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“…Procedures whereby BSA was immobilized to aminoderivatized silica gels via an intermediate reactive polymer layer have been developed by Millot and coworkers [87][88][89]. As illustrated in Fig.…”

Section: Immobilization Proceduresmentioning

confidence: 99%

“…Conditions: phosphate buffer, pH = 7.4 with 5% of 1-propanol; flow rate: 1 ml/min; temperature, 20 • C, UV detection at 280 nm (reproduced from[89] with permission, no further details on detection available).…”

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confidence: 99%