Binding of Host‐Associated Treponeme Proteins to Collagens and Laminin: A Possible Mechanism of Spirochetal Adherence to Host Tissues

Umemoto, Toshihiko; Namikawa, Isamu

doi:10.1111/j.1348-0421.1994.tb01836.x

Cited by 34 publications

(43 citation statements)

References 38 publications

(35 reference statements)

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“…In order to obtain a pure culture, the procedure was repeated more than 10 times, and spirochetal colonies were picked from solid sterility test medium supplemented with horse serum (35). Later, TYGVS medium was used as the routine culture medium and an anaerobic chamber (85% N,, 10% H,, 5% COz) was used for anaerobic conditions (34,36).…”

Section: Methodsmentioning

confidence: 99%

“…After electrophoresis, the gel was stained with Coomassie brilliant blue R-250 or used for Western transfer. Western transfer of the antigens from the gel to a nitrocellulose membrane was performed as described previously (19,34). An immunoblotting analysis was carried out by using goat anti-rabbit immunoglobulin G conjugated with peroxidase as the second antibody as described previously (19).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Treponema medium sp. nov., Isolated from Human Subgingival Dental Plaque

Umemoto

Nakazawa

Hoshino

et al. 1997

International Journal of Systematic Bacteriology

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A new Treponema species, for which we propose the name Treponema medium, was isolated from subgingival plaque from an adult with periodontal disease. The morphological characteristics, differential biochemical characteristics, and protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of this organism are described. The guanine-plus-cytosine content of the DNA of T. medium is 51 mol%. The levels of DNA-DNA relatedness of the new species to other Treponema species, including Treponema denticola, Treponema vincentii, Treponema socranskii, Treponema pallidum, and Treponema phagedenis, are less than 30%. A phylogenetic analysis based on 16s rRNA sequences distinguished the new Treponema strain from strains belonging to previously described Treponema species. The type strain of T. medium is strain G7201.The incidence and numbers of treponemes in the human gingival flora become greater as gingivitis becomes more severe (27). The number of treponemes increases to approximately more than 50% of all bacterial morphotypes in subgingival samples from periodontitis patients (10,12,13,27). Some oral treponemal species have been associated with soft-tissue damage due to proteolytic enzymes or with inflammatory reactions due to antibodies against their specific antigens, and eventually this leads to periodontal tissue destruction in certain types of periodontal disease (12,18,21,36). Simonson et al., for instance, have reported that Treponema denticola is linked to the severity of periodontal disease (25). All of the human oral spirochetes mentioned above belong to the genus Treponema in the family Spirochaetaceae; these bacteria characteristically are tightly coiled organisms that consist of a protoplasmic cylinder (length, approximately 6 to 20 pm; width, 0.1 to 0.5 pm) covered by an envelope (an outer sheath) and periplasmic flagella (axial flagella) which originate subterminally at the ends of the protoplasmic cylinder (26). Choi et al. used a 16s rRNA gene cloning technique to document the qualitative nature of human oral spirochetes and demonstrated that there is great genetic diversity in oral spirochetes, even in a single periodontitis patient (2). Human oral spirochetes, however, have been conventionally grouped into the following three morphotypes: (i) small spirochetes, including T. denticola, Treponema socranskii, and Treponema pectinovorum; (ii) medium-sized spirochetes, such as Treponema vincentii; and (iii) large spirochetes which have not been isolated in pure culture (3, 11). related spirochete with different periodontal status and reported that human oral spirochetes were not uniformly distributed within the dentition or around individual teeth T. denticola, T. socranskii, T. pectinovorum, T. vincentii,We isolated Treponema sp. strain G7201T (T = type strain), which could not be classified in any previously described species. We examined the phenotypic, serologic, and genetic characteristics of this organism and compared the results with data for reference species.In this paper we ...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Treponema medium sp. nov., Isolated from Human Subgingival Dental Plaque

Umemoto

Nakazawa

Hoshino

et al. 1997

International Journal of Systematic Bacteriology

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“…The adherence of human oral spirochetes to host tissues has been reported (21,23). T. socranskii possesses collagen-and laminin-binding polypeptides (23). In this study, T. socranskii was detected more often in subgingival plaque samples than in saliva samples.…”

Section: Periodontitismentioning

confidence: 58%

“…Therefore, further study will be necessary to make clear the relationship between the presence of T. socranskii in health-associated plaque and risk for periodontitis . The adherence of human oral spirochetes to host tissues has been reported (21,23). T. socranskii possesses collagen-and laminin-binding polypeptides (23).…”

Section: Periodontitismentioning

confidence: 99%

Detection of Treponema socranskii Associated with Human Periodontitis by PCR

Sakamoto

Takeuchi

Umeda

et al. 1999

Microbiology and Immunology

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A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells . Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis .

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“…Fenno et al (17) also found that recombinant Msp did not show an oligomeric form but it adhered to fibronectin and laminin. Umemoto et al (84,86) reported that T vincentii and T socranskii subsp. huecale have outer envelope proteins that bind to laminin, and that the 95 kDa outer sheath protein of T medium binds to type IV collagen.…”

Section: Adherencementioning

confidence: 99%