Binding of glycosaminoglycan inhibitors to calcium oxalate crystals in relation to ionic strength

Fellström, Bengt; Lindsjö, Monica; Danielson, Bo G.; Karlsson, F. Anders; Ljunghall, Sverker

doi:10.1016/0009-8981(89)90002-8

Cited by 18 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apparently some macromolecules have higher affinity for crystal surfaces and bind firmly. As a result they become occluded in the crystals after being adsorbed on their sur faces [4,34]. On the other hand, some macromolecules affect crystallization without firmly binding to crystals and becoming a part of their matrix.…”

Section: Crystal Nucleation and Growthmentioning

confidence: 99%

Interactions between Stone-Forming Calcific Crystals and Macromolecules

Khan¹

1997

Urol Int

View full text Add to dashboard Cite

Section: Crystal Nucleation and Growthmentioning

confidence: 99%

Interactions between Stone-Forming Calcific Crystals and Macromolecules

Khan¹

1997

Urol Int

View full text Add to dashboard Cite

“…It has been reported that the effect of macromolecular inhibitors on calcium oxalate crystallisation can change in accordance with ambient circumstances (Scurr and Robertson, 1986b;Fellstrom et al, 1989). The most likely mechanism employed by macromolecular inhibitors is that they are adsorbed onto the crystal surface (Angel and Resnick, 1989) Mean crystal size of calcium oxalate crystals in buffered 1986a).…”

Section: Discussionmentioning

confidence: 99%

Influence of Ionic Strength on Crystal Adsorption and Inhibitory Activity of Macromolecules

Utsunomiya

Koide

Yoshioka

et al. 1993

British Journal of Urology

View full text Add to dashboard Cite

We investigated the influence of ionic strength on both the binding ability of macromolecules onto calcium oxalate crystals and the inhibitory activity of macromolecules related to calcium oxalate crystal growth and aggregation in vitro. The amount of heparin sodium salt adsorbed onto calcium oxalate crystals was studied in both a seed and a non-seed crystal system at various ionic strengths with the aid of radiolabelled heparin. In both adsorption models, adsorption of heparin was greater in the buffered solutions of lower ionic strength, and significantly so in the range of physiological ionic strength. The inhibitory activity of heparin in the non-seed crystal system, which was determined using a Coulter Multisizer, increased as the adsorbed dose increased. The naturally existing urinary macromolecules showed a similar change in their inhibitory activity on calcium oxalate crystal growth and/or aggregation in accordance with changes in ionic strength. These results indicate that macromolecular inhibitory activity seems to be enhanced in urine of lower ionic strength as a result of an increased adsorption of macromolecules on the surface of calcium oxalate crystals.

show abstract

“…Besides low molecular weight substances, certain macromolecules may also act as inhibitors of calcium oxalate crystal aggregation [3,4]. Although in vitro studies have demonstrated that glycosaminoglycans (GAGs) are inhibitors of calcium oxalate crystal growth and aggregation in inorganic reaction media [5][6][7][8], there is little evidence that this activity is retained in urine. Thus, Correspondence to: E Harangi, Kerpel-Fronius 0d0n Children's Hospital of Baranya County, H-7601 P6cs, Pf.76., Hungary whether or not urinary GAGs are inhibitors of stone formation remains a matter of controversy, and the issue has been further complicated by conflicting data from in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

Urinary glycosaminoglycan excretion in healthy and stone-forming children

1996

View full text Add to dashboard Cite

Both in vivo and in vitro studies suggest that macromolecules excreted in the urine, e.g. glycosaminoglycans (GAGs) may be inhibitors of kidney stone formation. We evaluated urinary GAG excretion in 22 children with calcium oxalate stones [8 with absorptive hypercalciuria, 6 with renal hypercalciuria (RH), 8 with normocalciuria], and in 20 age-matched controls. There was no significant difference between the two groups in the total urinary GAG level. In terms of the various GAG fractions, patients with RH excreted considerably less keratan sulphate and considerably more dermatan sulphate than the other patients and healthy controls. There was no difference between the two groups in condroitin sulphate, heparan sulphate and hyaluronic acid excretion. We conclude that there is no significant correlation between the formation of calcium oxalate stones and urinary GAG excretion.

show abstract

Binding of glycosaminoglycan inhibitors to calcium oxalate crystals in relation to ionic strength

Cited by 18 publications

References 11 publications

Interactions between Stone-Forming Calcific Crystals and Macromolecules

Interactions between Stone-Forming Calcific Crystals and Macromolecules

Influence of Ionic Strength on Crystal Adsorption and Inhibitory Activity of Macromolecules

Urinary glycosaminoglycan excretion in healthy and stone-forming children

Contact Info

Product

Resources

About