Binding of fibronectin to Staphylococcus strains

Abstract

Fibronectin, a major protein component of plasma and loose connective tissue has previously been shown to bind to several strains of Staphylococcus aureus. We examined a large number of strains of different species of Staphylococcus with respect to their ability to bind fibronectin. The relative numbers of strains defined as fibronectin-binders among the different species were as follows: S. aureus (22 of 23), S. haemolyticus (5 of 5), S. warneri (8 of 11), S. hyicus (5 of 6), S. hominis (13 of 17), S. saprophyticus (11 of 20), S. epidermidis (4 of 7), and S. simulans (8 of 10). Only three species showed a predominance of nonbinders over binders: S. capitis (4 of 14), S. xylosus (0 of 4), and S. cohnii (3 of 11). These data indicate that staphylococcal species isolated from soft tissue infections frequently have the ability to bind fibronectin and suggest that the ability to bind to this protein may contribute to the virulence of coagulase-positive and coagulase-negative staphylococci.

Fibronectin concentrations in catheter sepsis

“…The strains which caused the catheter sepsis were of species reported to have receptors for fibronectin on their surfaces, while those which colonized catheters were slimenegative strains, which are believed not to bind fibronectin. These results are in agreement with those reported by Switalski et al [12]. At present, however, this remains only a hypothesis, which is worthy of further investigation.…”

Vitronectin and type-I collagen binding by Staphylococcus aureus and coagulase-negative staphylococci

“…This strain shows depressed levels of extracellular products, but elevated levels of cell-bound proteins, such as coagulase and protein A [28,29]. Coagulase-negative staphylococcal reference strains previously characterized for Fn and Type-ll Cn binding were included in this study [21]. S. haemolyticus strain E 2498/86 was isolated from osteomyelitis.…”

“…intermedius and $. hyicus), while fibronectin and Type-I and -II collagen bind to S. aureus and coagulase-negative staphylococci associated with human and animal infections [21,22]. Fuquay et al [23] first reported binding of human serum spreading factor (i.e.…”

Characterization of fibronectin binding to Salmonella enteritidis strain 27655R

“…The characterisation of fibronectin binding by this strain showed 2 binding sites; one of high-affinity (K a = 5.8 x 10 -l° M) and one of low affinity (K d = 2.0 × 10 -8 M). The low-affinity binding site in this strain has a similar K d to the low-affinity binding site identified for E. coil [8], whereas the high-affinity binding site has a K d of the same efficiency as those described for staphylococci and streptococci [3,4].…”

“…440 000 kDa) involved in eukaryotic cell adhesion and spreading [1]. Recent studies suggest that a number of bacterial pathogens, including staphylococci and streptococci [2][3][4][5], as well as Treponema pallidum [6], have the capacity to bind to fibronectin [7]. Strains of enterotoxigenic Escherichia coli (ETEC) of the 06 serogroup have been shown to interact with fibronectin at two binding sites, one of which is situated within the 29-kDa N-terminal domain [8].…”