Fibronectin concentrations in catheter sepsis

Nemet, Branka; Zagar, Z

doi:10.1046/j.1469-0691.2000.00029.x

Cited by 7 publications

(2 citation statements)

References 16 publications

(21 reference statements)

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“…Matricellular proteins, including vitronectin, fibronectin, and fibrinogen, have previously been shown to bind to C. albicans, likely augmenting invasion of various tissues (16)(17)(18)(19)(20)(21)(22)(23). These proteins consistently associate with biofilms of medical devices and have been studied as host-conditioning proteins prior to biofilm initiation (24)(25)(26)(27)(28)(29). In this study, we demonstrate that fibronectin is influential for adhesion to plastic and that targeting this interaction reduces biofilm formation.…”

Section: Figmentioning

confidence: 59%

Targeting Fibronectin To Disrupt In Vivo Candida albicans Biofilms

Nett

Cabezas-Olcoz

Marchillo

et al. 2016

Antimicrob Agents Chemother

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New drug targets are of great interest for the treatment of fungal biofilms, which are routinely resistant to antifungal therapies. We theorized that the interaction of Candida albicans with matricellular host proteins would provide a novel target. Here, we show that an inhibitory protein (FUD) targeting Candida-fibronectin interactions disrupts biofilm formation in vitro and in vivo in a rat venous catheter model. The peptide appears to act by blocking the surface adhesion of Candida, halting biofilm formation.

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Section: Figmentioning

confidence: 59%

Targeting Fibronectin To Disrupt In Vivo Candida albicans Biofilms

Nett

Cabezas-Olcoz

Marchillo

et al. 2016

Antimicrob Agents Chemother

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“…C. albicans is known to interact with these ubiquitous matricellular proteins through specific interactions, which may enhance tissue invasion and contribute to virulence (43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54). Given the evidence for the likely involvement of these proteins in Candida pathogenicity, pioneering biofilm investigations have emphasized the importance of a host conditioning fluid for optimal biofilm formation and the need to account for the proteins in in vitro biofilm models (29,35,(55)(56)(57)(58). However, little is known about how assimilation of host proteins may affect biofilm structure and function, perhaps even fostering biofilm development.…”

Section: Discussionmentioning

confidence: 99%

Host Contributions to Construction of Three Device-Associated Candida albicans Biofilms

et al. 2015

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e Among the most fascinating virulence attributes of Candida is the ability to transition to a biofilm lifestyle. As a biofilm, Candida cells adhere to a surface, such as a vascular catheter, and become encased in an extracellular matrix. During this mode of growth, Candida resists the normal immune response, often causing devastating disease. Based on scanning electron microscopy images, we hypothesized that host cells and proteins become incorporated into clinical biofilms. As a means to gain an understanding of these host-biofilm interactions, we explored biofilm-associated host components by using microscopy and liquid chromatography-mass spectrometry. Here we characterize the host proteins associated with several in vivo rat Candida albicans biofilms, including those from vascular catheter, denture, and urinary catheter models as well as uninfected devices. A conserved group of 14 host proteins were found to be more abundant during infection at each of the niches. The host proteins were leukocyte and erythrocyte associated and included proteins involved in inflammation, such as C-reactive protein, myeloperoxidase, and alarmin S100-A9. A group of 59 proteins were associated with both infected and uninfected devices, and these included matricellular and inflammatory proteins. In addition, site-specific proteins were identified, such as amylase in association with the denture device. Cellular analysis revealed neutrophils as the predominant leukocytes associating with biofilms. These experiments demonstrate that host cells and proteins are key components of in vivo Candida biofilms, likely with one subset associating with the device and another being recruited by the proliferating biofilm.

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