Binding of DNA-binding alkaloids berberine and palmatine to tRNA and comparison to ethidium: Spectroscopic and molecular modeling studies

Drug–DNA binding thermodynamics: A comparative study of aristololactam-β-d-glucoside and daunomycin

“…(283. 15, 293.15, 303.15, and 313.15) K as described in details elsewhere [27]. DNA and drug solutions were degassed on the MicroCal's Thermovac unit before loading to avoid the formation of bubbles in the calorimeter cell.…”

Biophysical studies on the base specificity and energetics of the DNA interaction of photoactive dye thionine: Spectroscopic and calorimetric approach

“…A MicroCal VP-ITC unit (MicroCal, Inc., Northampton, MA, USA) using protocols developed in our laboratory and described in details previously [25,30,35,36] was used for all ITC experiments. A reverse titration protocol of injecting aliquots of DNA solution from a rotating syringe (290 rpm) into the calorimetric cell containing the thionine solution (1.4235 mL) under identical buffer conditions was adopted.…”

“…A large ΔCp value is usually associated with changes in hydrophobic or polar group hydration and considered as indicator of a dominant hydrophobic effect in the binding process. Negative heat capacity values have been observed for a variety of small molecules binding to DNA and RNA [21,23,27,35,42]. Change in solvent accessible surface area has been shown to be large component of ΔCp [44][45][46].…”

A thermodynamic investigation on the binding of phenothiazinium dyes azure A and azure B to double stranded RNA polynucleotides

“…Titrations were performed at three temperatures, namely 283.15, 293.15 and 303.15 K by using the protocol described previously [22]. …”