Binding of Dihydrotestosterone, R 1881 and R 5020 in Cytosols from Normal, Benign Hypertrophic and Cancerous Human Prostates

Abstract

The binding of dihydrotestosterone, R 1881 and R 5020 as examined in cytosols of normal, benign hypertrophic and cancerous tissues of the human prostates. Almost all samples obtained by open operation showed high affinity binding to these ligands. Dissociation constants of the binding to these ligands were approximately 10^––9M irrespective of the pathological state of the prostates. Maximum binding sites for dihydrotestosterone seemed to be greater in normal tissues than in the pathological ones. However, maximum binding sites for R 1881 and R 5020 were not significantly different among the normal and pathological prostates examined in the present study. Moreover, some correlation was observed between the maximum binding sites for R 1881 and those for R 5020. The samples resected by TUR seemed to be inadequate for analyses of androgen binding.

Influence of Surgical Techniques on Receptor Levels and 5α-Reductase Activity of the Human Prostate Gland

“…Nonetheless there are still many investigators who believe that TUR material should not be used for receptor assay under any circumstances [1,2]. However, in an attempt to identify the technical factors limiting the use of TUR, Albert et a1 171 recognized that the size of the resectoscope and the type of current frequency used were crucial to the quantitation of androgen receptors in TUR chips; specimens ?The size (length x thicknedwidth) of the large TUR chips (six) was greater than 1.5 x 0.6 cm whereas the small chips (six) were less than 1.5 X 0.4 cm.…”

The type of current frequency used in transurethral resection of prostate (TURP) affects the androgen receptor

“…Snochowski et a1 [ 11 reported that hyperplastic specimens removed by electroresection were devoid of androgen receptor, and that only tissue samples removed by transvesical resection or other noncoagulative procedures should be used for receptor assays. Recently Harper et a1 [2] and Nozumi et a1 [3] have also stated that TURP samples are unsuitable for receptor assays. Since open surgical prostate resection is performed infrequently in many parts of the United States, it was considered important for those studying human prostate androgen receptor to know the technical factors limiting the use of TURP tissue.…”

Characteristics of Progestin Binder in Hypertrophic Human Prostate.

“…It has been reported repeatedly that the human prostate contains a specific progestin-binding component which shows high affinity and low capacity binding with progestational steroids (Cowan et al, 1977, Shoran and Boesel, 1978, Nozumi et al, 1981. The binder has a similar Kd (dissociation constant) and sedimentation coefficient on sucrose gradient centrifugation to those of the progesterone receptor in the human uterine endometrium (Robel et al, 1981) and in the breast cancer (Horwitz et al, 1985), therefore, the binder in the human prostate has been assumed to be the progesterone receptor per se (Gustafsson et al, 1978, Ekman et al, 1982, Bashirelahi et al, 1983, Schneider et al, 1984.…”