The type of current frequency used in transurethral resection of prostate (TURP) affects the androgen receptor

Abstract

Blended Bovie current No. 2 destroys androgen receptor in both cytosol and nuclear extracts of prostate tissue. No significant difference was noted in either nuclear or cytosol receptor concentrations between openly resected and electroresected specimens when Bovie current No. 1 was used with cutting loops No. 26 or larger, although total androgen receptor (cytosol plus nuclear) was significantly reduced in the electroresected specimens. The androgen receptor method used is highly reliable for prostate obtained by either open resection or electroresection using pure cutting currents, although receptor concentrations in samples obtained by the two methods probably should not be combined or compared.

Acute therapy with megestrol acetate decreases nuclear and cytosol androgen receptors in human BPH tissue

“…This current from the Rf tube circuit of 3.2 megacycles is an undampened, clean wave form (narrow frequency range) providing a pure cut of tissue without coagulation. We have previously shown [8] that values for nuclear and cytosol receptor in tissue obtained by TURP using this current do not significantly differ from values obtained in openly resected prostates. After cleaning and mincing the prostate tissue, samples were either homogenized, extracted and measured for receptor content in cytosol and nuclear extracts the same day or were packaged in parafilm and frozen at -80°C for extraction and measurement at a later date after up to five months of storage.…”

Influence of Surgical Techniques on Receptor Levels and 5α-Reductase Activity of the Human Prostate Gland

“…Previous studies [7] comparing hormone receptor levels in TUR specimens removed with different resection loops have revealed that there was no significant difference in mean cytosol or nuclear androgen receptor values for tissues obtained either by open surgery or TUR when a large cutting loop with high-frequency current was used. However, when the total cellular androgen receptor concentrations were compared, the levels in the resected specimens were found to be significantly lower than those measured in the open specimens.…”

Evaluation of Biopsy Techniques for Androgen Receptor Assay in Human Prostatic Tissue

“…This situation has been examined by some workers, some of whom consider that it is a handicap but not an objection to receptor assay Accepted for publication 18 March 1985 using resector tissue, particularly when chippings are sufficiently large to permit removal of external charring (Snochowski et al, 1977;Bashirelahi et a[., 1980;Ghanadian and Auf, 1980;Nozumi et al, 1981). The frequency of current used for resection can also influence results of receptor assays (Albert et al, 1982). The alternative technique for removal of prostatic tissue endoscopically is cold punch resection, which is unlikely to expose the interior of resected chippings to any physical trauma.…”