Binding interaction between aloe polysaccharide and alizarin red by spectrophotometry and its analytical application

Gu, Wenxing; Song, He; Wen, Xiaonan; Wang, Yingying; Xia, Wenshui; Fang, Yun

doi:10.1016/j.carbpol.2009.10.074

Cited by 8 publications

(2 citation statements)

References 29 publications

(23 reference statements)

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“…Ethanol insoluble residues were separated from A. vera and A. marlothii gel materials according to a method previously described. [ 14 15 ] The starting material for Aloe vera was dehydrated gel powder (Daltonmax 700 ® ) obtained from Improve USA, Inc. (Texas, United States of America). The starting material for Aloe marlothii was obtained from natural populations near Koster in the North West Province of South Africa for which a specimen voucher (PUC 11151) was deposited at the Herbarium of the North-West University, South Africa.…”

Section: Methodsmentioning

confidence: 99%

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

et al. 2014

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Objective:To investigate the skin hydrating and anti-erythema activity of gel materials from Aloe marlothii A. Berger and A. ferox Mill. in comparison to that of Aloe barbadensis Miller (Aloe vera) in healthy human volunteers.Materials and Methods:Aqueous solutions of the polisaccharidic fractions of the selected aloe leaf gel materials were applied to the volar forearm skin of female subjects. The hydration effect of the aloe gel materials were measured with a Corneometer® CM 825, Visioscan® VC 98 and Cutometer® dual MPA 580 after single and multiple applications. The Mexameter® MX 18 was used to determine the anti-erythema effects of the aloe material solutions on irritated skin areas.Results:The A. vera and A. marlothii gel materials hydrated the skin after a single application, whereas the A. ferox gel material showed dehydration effects compared to the placebo. After multiple applications all the aloe materials exhibited dehydration effects on the skin. Mexameter® readings showed that A. vera and A. ferox have anti-erythema activity similar to that of the positive control group (i.e. hydrocortisone gel) after 6 days of treatment.Conclusion:The polysaccharide component of the gel materials from selected aloe species has a dehydrating effect on the skin after multiple applications. Both A. vera and A. ferox gel materials showed potential to reduce erythema on the skin similar to that of hydrocortisone gel.

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Section: Methodsmentioning

confidence: 99%

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

et al. 2014

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“…Various solvents/mediums had been used for the esterification including water, organic solvents such as pyridine, DMSO, dioxane and toluene, and DMAc/LiCl . Recently, to improve the sustainability of the route and eliminate the use of harmful organic solvents, some new “green solvent” such as supercritical carbon dioxide (Sc CO 2 ) and ionic liquid (IL), was adopted and studied for the starch esterification as well as new catalysts such as enzyme . However, the use of Sc CO 2 required high‐pressure equipments and processes and the ILs were relatively expensive, which seriously limited their applications.…”

Section: Esterification Conditions and Degree Of Substitution (Ds) Ofmentioning

confidence: 99%

Enzyme‐Catalyzed Starch Esterification in Deep Eutectic Solvent

Deng

Han

et al. 2019

ChemistrySelect

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For the first time the enzymatic esterification of starch in deep eutectic solvent (DES) was reported using DES of choline chloride (ChCl)‐ethylene glycol (EG) as reaction solvent/medium, Novozyme 435 as catalyst, and PEG 400 as phase transfer agent to synthesize starch decanoate, starch laurate, and starch palmitate. The product samples of esterified starches were characterized by FTIR, 1H NMR, GPC, SEM, XRD, and DSC tests. The results showed that, the degrees of substitution were in the range of 0.07‐0.19 and the addition of PEG 400 was helpful to increase the DS. The optimal esterification temperature for DS was 338 K in the range studied. The degradation of starch was found to be minor due to the use of long‐chain fatty acid and relatively low reaction temperatures. The dissolution, gelatinization, esterification, and recrystallization of starch in DES broke the particles into smaller ones and altered the crystal structure of native starch while the aggregation between particles was increased and the degree of crystallinity was decreased for the esterified starch with the length of carbon‐chain in the fatty acids.

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A Novel Fluorescence Quenching Method for the Determination of Aloe Polysaccharide

Gu¹,

Song²,

Wen³

et al. 2011

Chin. J. Chem.

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Alizarin red (AR) can bind with aloe polysaccharide (APS) in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The chromogenic reaction is rapid and the fluorescence intensity remains stable for at least 2 h at room temperature. for APS and the mean recovery (100.2±2)%, RSD≤3%. The effect of various substances on the determination of APS was also investigated in detail, and the results show that most of the studied coexistent substances can be tolerated in considerable amounts. The proposed method is sensitive, simple, fast and suitable for routine assay.

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Binding interaction between aloe polysaccharide and alizarin red by spectrophotometry and its analytical application

Cited by 8 publications

References 29 publications

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications

Enzyme‐Catalyzed Starch Esterification in Deep Eutectic Solvent

A Novel Fluorescence Quenching Method for the Determination of Aloe Polysaccharide

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