Binding activity of avidin to the biotin within mesoporous silica materials for bioanalytical applications

Orita, Toru; Tomita, Masahiro; Harada, Megumi; Kato, Katsuya

doi:10.1016/j.ab.2012.02.037

Cited by 13 publications

(5 citation statements)

References 43 publications

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“…The surface of GdPB-A488 is then modified with the biotinylated anti-human eotaxin-3 antibody using avidin–biotin interactions (Figure A). The interaction between avidin and biotin is one of the strongest noncovalent interactions known ( K d = 10 –15 M) − and can withstand extremes of temperature, pH, and denaturing agents. − Anti-human eotaxin-3 antibody specifically binds to antigens on the eosinophilic cell line (EoL-1) conferring molecular targeting capabilities to GdPB. By using avidin–biotin interactions, we ensure robust attachment of the targeting antibody and maximize the efficiency and selectivity of the biofunctionalized nanoparticles for molecular imaging.…”

Section: Discussionmentioning

confidence: 99%

Biofunctionalized Gadolinium-Containing Prussian Blue Nanoparticles as Multimodal Molecular Imaging Agents

et al. 2013

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Molecular imaging agents enable the visualization of phenomena with cellular and subcellular level resolutions and therefore have enormous potential in improving disease diagnosis and therapy assessment. In this article, we describe the synthesis, characterization, and demonstration of core-shell, biofunctionalized, gadolinium-containing Prussian blue nanoparticles as multimodal molecular imaging agents. Our multimodal nanoparticles combine the advantages of MRI and fluorescence. The core of our nanoparticles consists of a Prussian blue lattice with gadolinium ions located within the lattice interstices that confer high relaxivity to the nanoparticles providing MRI contrast. The relaxivities of our nanoparticles are nearly nine times those observed for the clinically used Magnevist. The nanoparticle MRI core is biofunctionalized with a layer of fluorescently labeled avidin that enables fluorescence imaging. Biotinylated antibodies are attached to the surface avidin and confer molecular specificity to the nanoparticles by targeting cell-specific biomarkers. We demonstrate our nanoparticles as multimodal molecular imaging agents in an in vitro model consisting of a mixture of eosinophilic cells and squamous epithelial cells. Our nanoparticles specifically detect eosinophilic cells and not squamous epithelial cells, via both fluorescence imaging and MRI in vitro. These results suggest the potential of our biofunctionalized Prussian blue nanoparticles as multimodal molecular imaging agents in vivo.

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Section: Discussionmentioning

confidence: 99%

Biofunctionalized Gadolinium-Containing Prussian Blue Nanoparticles as Multimodal Molecular Imaging Agents

et al. 2013

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“…Previous studies have demonstrated that proteins (smaller than the pore size) tend to adsorb inside the wall of MPS by a capillary phenomenon. 30,31 Based on the above results, it is suggested that protein A was immobilised within the pore of MPS. In other words, MPS-2.7, with a pore size similar to or smaller than that of protein A, can conne protein A.…”

Section: Characterisationmentioning

confidence: 86%

“…29 Similarly, it has also been reported that avidin protein immobilised on the MPS pore gate exhibits remarkably high binding towards biotin and remains stable during hydrothermal or solvent (including HCl and MeOH) treatments. 30 This could be attributed to the existence of MPSs of different pore sizes and surface morphologies, which enhanced the activity of immobilised avidin. These results indicate that MPS could potentially be used as a protein carrier.…”

Section: Introductionmentioning

confidence: 99%

Specific binding of immunoglobulin G to protein A–mesoporous silica composites for affinity column chromatography

et al. 2013

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“…Immobilization onto inert inorganic materials may cope with stability and compatibility problems, thus favoring advance in technological applications. The success of such a strategy depends on proper selection of the hybrid materials that can support or host the bioactive components while maintaining its activity and selectivity [22] [23]. Some examples of this application are:…”

Section: Porous Materials For a Drug Deliverymentioning

confidence: 99%

Micro, Meso and Macro Porous Materials on Medicine

Solano-Umaña¹,

Vega-Baudrit²

2015

JBNB

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An enormous development is experimented on the porous materials. The porous was considered a defect on solid materials some years ago, but right now, this defect is an advantage, based on the properties obtained from a micro and mesoporous materials. Microporous, mesoporous and macroporous materials with an uniformed pore distribution offer new properties, such as absorption, adsorption, exchange separation, and catalysis of different compounds, also they can play different roles as hosts for a nanocomposite materials to modify or improve their properties. Today, the structural types of open framework porous compounds have rapidly increased by their unique structural properties, porous size window and accessible void space are critical factors on a medical application.

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Binding activity of avidin to the biotin within mesoporous silica materials for bioanalytical applications

Cited by 13 publications

References 43 publications

Biofunctionalized Gadolinium-Containing Prussian Blue Nanoparticles as Multimodal Molecular Imaging Agents

Biofunctionalized Gadolinium-Containing Prussian Blue Nanoparticles as Multimodal Molecular Imaging Agents

Specific binding of immunoglobulin G to protein A–mesoporous silica composites for affinity column chromatography

Micro, Meso and Macro Porous Materials on Medicine

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