Specific binding of immunoglobulin G to protein A–mesoporous silica composites for affinity column chromatography

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

“…The changes of the three-dimensional structures of GOX by the adsorption on pLys-HAp samples were conrmed by ANS method. [34][35][36][37] GOX (500 mg) was immobilised on a-pLys-HAp (40 mg) and 3-pLys-HAp (40 mg) during the overnight stirring at 20 C. Furthermore, ANS solution (50 mg mL À1 in 10 mM phosphate buffer at pH 7.0) was added to the suspension. The mixture was incubated at 20 C for 3 h in the dark.…”

“…These results indicated that the conformational change in GOX occurred due to the GOX immobilisation on pLys-HAp. 37 3.5 Enzymatic kinetics and the catalytic activity of GOX on pLys-HAp Fig. S4 † shows the catalytic activities of GOX (free and immobilised on pLys-HAp).…”

Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture

“…The optimization of the structure and surface characteristics of MPS facilitates its use as an adsorption material. For example, MCM-41, which is surface functionalized with glycidoxy groups, exhibited enhanced binding efficiency and stability as a protein carrier in antibody purification chromatography [23]. Organo-functionalized MPS fabricated for biosensors enhances the catalytic activity and stability of immobilized enzymes [24,25].…”

Elucidating the effect of different amino-functionalized spherical mesoporous silica characteristics on ribonucleic acid selectivity and adsorption capacity

“…We previously reported that the pore diameter of MPS affects the protein and DNA adsorption capacities [18,21]. The diameter of the DNA double strand is approximately 2 nm [30], which may be related to the low DNA adsorption capacities observed in the present study.…”

“…Previously, we focused on silica morphology and surface functional groups, and reported on their biological applications [17][18][19][20][21]. MPS with sheet morphology was found to improve the activity of cytochrome c by up to 178%, which indicated that it is important for sheet morphology to be used in protein carriers [19].…”