Binding ability of chitinase onto cellulose: an atomic force microscopy study

Atomic Force Microscopic Study of Chitinase Binding onto Chitin and Cellulose Surfaces

“…This was the case at the loading rate of 2 nN/s, as reported in the previous studies. 18,19 The most commonly used theoretical model for the single force spectroscopy, namely, Bell-Evans model 21,22 has predicted a linear relationship between the most probable binding force (F) and the logarithm of the loading rate (r), allowing the calculation of the effective bond length (x β ) and dissociation rate constants (k d ) from the following equation:…”

“…16 Then, binding characteristics of ChBD1 and ChBD2 toward chitin and cellulose had been studied by the force curve measurements using AFM (Figure 1A); however, the analysis was conducted at a single loading rate. 18,19 Therefore, the loading rate dependencies would provide the full description of the binding mechanisms of the chitinase onto polysaccharide surfaces.…”

“…Most of the polysaccharide degrading enzymes contain the substrate-binding modules, which support the increase of the effective density of the enzymes on the polysaccharide surfaces. , In the present study, we focus on the chitinase secreted from the hyperthermophilic archaeon, Thermococcus kodakarensis KOD1, which is composed of two catalytic domains (CatDA, CatDB) and three substrate binding domains (ChBD1, ChBD2, ChBD3). , Concerning the binding domains, ChBD2 and ChBD3 have almost identical amino acid sequences (85% identity, 92% similarity within 100 amino acids), whereas ChBD1 has quite different sequence from the other ChBDs . Then, binding characteristics of ChBD1 and ChBD2 toward chitin and cellulose had been studied by the force curve measurements using AFM (Figure A); however, the analysis was conducted at a single loading rate. , Therefore, the loading rate dependencies would provide the full description of the binding mechanisms of the chitinase onto polysaccharide surfaces.…”

Enzymatic Systems for Cellulose Acetate Degradation

“…The N-acetamide group seems to be essential for the ability of chitinase to detect glycosidic bonds. The binding ability for a chitinase on chitin and cellulose was determined to be equal for both polymers [38]. The effects of reduced binding affinity to substrates with at least one acetyl group per monomer might be the reason for reduced backbone cleavage of cellobiohydrolase for CA 1.4 and CA 1.8.…”

Structure prediction and network analysis of chitinases from the Cape sundew, Drosera capensis

“…44 AFM data suggests the binding is mostly determined by interaction of the aromatic residues in the binding site (orange in Figure 2E) with the pyranose rings of the substrate. 45 This type of functionality has not been previously observed in plants; we hypothesize that it is an adaptation associated with carnivory, perhaps related to more effective breakdown of small oligosaccharides to components that can be used as a nitrogen source.…”