This work demonstrates how computational techniques can facilitate quickly moving from raw sequence data to refined structural models and comparative analysis, and to select promising candidates for subsequent biochemical characterization. This capability is increasingly important given the large and growing body of data from high-throughput genome sequencing, which makes experimental characterization of every target impractical.

Background: Visual impairment, specifically anterior segment pathology, presents a significant burden of disease in the world. Introduction: Inexpensive tools are necessary to improve eye health of residents in developing countries where care is difficult to access. Our study aimed at determining whether a $5 macro lens attached to a smartphone camera is an effective anterior segment imaging method for screening diseases. Materials and Methods: Fifty four (n = 54) patients had anterior segment imaging performed by using an Easy Macro lens and an iPhone. Imaging was performed at the Floating Doctors' mobile clinic sites in Panama. Images were sent back and graded by two board-certified ophthalmologists using a modified version of the FOTO-ED scale. Statistical analysis was performed by using a Wilcoxon signed-rank test to compare grades between the two imaging modalities. Results: There was no significant difference in overall clinical utility of images obtained by the iPhone versus Easy Macro lens. The iPhone was significantly superior in imaging of the lens and conjunctiva, whereas the Easy Macro lens was superior in regards to the anterior chamber, iris, and lens. Discussion: The imaging modality that best captures pathology is dependent on what part of the anterior segment is being examined. An imaging protocol with a pair of images, one from a smartphone and one from a macro lens, would have significant clinical utility. Conclusion:Our study demonstrates how minimally trained users can deliver effective eye screening via a telemedicinebased approach in a resource-deprived setting. Future directions would be to develop a telemedicine protocol and determine whether it improves clinically measurable outcomes in patients.

Background: Panama has a high incidence of tuberculosis (TB), especially in rural communities. A patient survey was administered to assess practices, knowledge and attitudes towards TB within the Ngabe-Bugle population. Methods: A cross-sectional survey was distributed at Floating Doctors clinics. Subjects with familiarity of TB were assessed through questions about transmission, cause, symptoms, and treatment. Results: Of the 106 patients who completed the TB practices survey, 68 patients (64%) knew of TB and completed the entire survey. Of the 64% who knew of TB, 61% knew medicine treated TB and over 80% could identify symptoms of TB. 40% reported they would feel ashamed of a TB diagnosis. Conclusions: Most with TB familiarity knew of TB symptoms and cause, but were less informed about mode of transmission and treatment. The majority of subjects had negative attitudes towards TB. This shows a need to expand TB education in the Ngabe-Bugle communities.

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