Bilirubin oxidase as a single enzymatic oxygen scavenger for the development of reductase-based biosensors in the open air and its application on a nitrite biosensor

One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions

“…Catalase, glucose oxidase, and protocatechuate dioxygenase represent the most common O 2 -scavenging enzymes. , However, in our case, their substrates/products (i.e., gluconolactone and protocatechuate, respectively) are UV–vis active, which affects the optical analysis for monitoring the [4Fe-4S] cluster assembly. Recently, bilirubin oxidase has been used as a single-enzyme O 2 -scavenger without H 2 O 2 generation . Unfortunately, bilirubin (red) and the oxidized product biliverdin (green) also affect the optical analysis of the [4Fe-4S] clusters.…”

“…Recently, bilirubin oxidase has been used as a single-enzyme O 2 -scavenger without H 2 O 2 generation. 46 Unfortunately, bilirubin (red) and the oxidized product biliverdin (green) also affect the optical analysis of the [4Fe-4S] clusters. Interestingly, the NADH-dependent flavin reductase (FRE) required for FADH 2 regeneration in the SUF system can also scavenge O 2 to form H 2 O 2 .…”

Emerging Trends of Bilirubin Oxidases at the Bioelectrochemical Interface: Paving the Way for Self-Powered Electrochemical Devices and Biosensors

“…Owing to its effective oxygen reduction capabilities without the production of hydrogen peroxide and its capacity to function effectively in the presence of ambient air, this novel monoenzymatic oxygen scavenging system holds promise for a variety of applications, particularly in biosensing and bioelectronic devices. 85 ORR is important because of its high electrochemical potential. MCOs, such as BOD, have gained attention for their ability to catalyze the ORR with little excess overpotential.…”

“…This nitrite biosensor showed good performance even under ambient air conditions, obviating the need for a second enzyme to prevent the buildup of intermediates reactive with oxygen. Owing to its effective oxygen reduction capabilities without the production of hydrogen peroxide and its capacity to function effectively in the presence of ambient air, this novel monoenzymatic oxygen scavenging system holds promise for a variety of applications, particularly in biosensing and bioelectronic devices …”

A (μ-oxo) dicopper complex anchoring graphitized mesoporous carbon surface prepared by an in situ electrochemical method for bioinspired electrocatalytic reduction of nitrite to ammonia and sensing

“…8 ccNiR catalyzes the nitrite to ammonium conversion via intramolecular binding of nitrite on a low-valent aquo-ligand containing iron site Fe(II), through a multi-electron-transfer pathway. 33,34 This reaction has been studied by electrochemical techniques using enzyme-modied electrodes, 35,36 which have also been explored for bioelectroanalytical applications [37][38][39] where the selectivity and sensitivity of detection have been shown to be major advantages. However, their mass production has been hindered by issues such as the cost of enzyme purication and the biosensor shelf-life.…”