Bilirubin inhibits cartilage metabolism and growth in vitro

Vassilopoulou‐Sellin, Rena; Oyedeji, Caroline O.; Samaan, Naguib A.

doi:10.1016/0026-0495(89)90062-0

Cited by 12 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it is noteworthy that micromolar concentrations of bilirubin have been shown to inhibit cartilage growth in vitro. 37 No significant difference was detected between groups for IFN-γ, IL-12p70, or IL-2, all of which were found to be absent in most samples.…”

Section: Discussionmentioning

confidence: 82%

Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 82%

Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Therefore we hypothesize that marked and sustained hyperbilirubinemia might have affected by itself the metabolism and/or structure of the connective tissue. Recently, bilirubin has been demonstrated to inhibit cartilage metabolism and growth in uitro (22). However, we cannot rule out the possibility that the skin disorder is coincidentally associated with congenital chronic hyperbilirubinemia.…”

Section: Discussionmentioning

confidence: 87%

Responsiveness to phenobarbital in an adult with crigler-najjar disease associated with neurological involvement and skin hyperextensibility

et al. 1991

View full text Add to dashboard Cite

We present the case of a 23-yr-old man who had had since birth marked and sustained unconjugated non-hemolytic hyperbilirubinemia and who had had several attacks of grand mal seizures. Analysis of serum bilirubin by diazoreactive methods showed serum levels of unconjugated bilirubin as high as 445 mumol/L that were not affected by phenobarbital administration. However, analysis of serum bile pigments by high-pressure liquid chromatography demonstrated marked decrease of unconjugated bilirubin after phenobarbital treatment (from 432.4 mumol/L to 291.0 mumol/L) associated with slight increase of bilirubin monoconjugates and disconjugates (from 0.25 mumol/L to 0.42 mumol/L). Furthermore, in the past few years the patient had exhibited striking skin hyperextensibility and diaphragm eventration. This case confirms that alkaline methanolysis-high-pressure liquid chromatography is the most reliable method for assessment of serum fraction bilirubin levels; that clinical parameters such as neurological signs do not unequivocally discriminate between type I and II Crigler-Najjar disease and that response to phenobarbital treatment remains the main diagnostic tool.

show abstract

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

Vassilopoulou‐Sellin

Rey-Bear

Oyedeji

1990

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

Elevated levels of bilirubin and other tetrapyrroles are common to a number of chronic hematologic and liver diseases that can result in growth failure. This report establishes a cellular model system for the study of these endogenous growth inhibitors. Primary chondrocyte cultures were prepared from embryonic chick cartilage; cells were incubated (0.3 X 10(5) cells per plate) in tissue culture medium containing 10% fetal bovine serum (FBS) with or without added bilirubin, 0.01-0.10 mM. Bilirubin caused profound, dose-dependent inhibition of chondrocyte proliferation: after 7 days, control incubations contained 11.45 +/- 2.0 X 10(5) cells per plate versus 4.92 +/- 0.55 X 10(5) cells per plate for wells with added 0.01 mM bilirubin and 1.76 +/- 0.30 X 10(5) for cultures with added 0.05 mM bilirubin. In chondrocytes cultured for 3 days, the addition of 0.05 mM bilirubin was associated with inhibition of [3H]thymidine incorporation into DNA (47 +/- 5% of control), [3H]uridine incorporation into RNA (11 +/- 0.05% of control), and [14C]leucine incorporation into proteins (16 +/- 1% of control). The inhibition of chondrocyte proliferation induced by a range of bilirubin concentrations (0.01-0.10 mM) was in no way attenuated by the addition of physiologic concentrations of albumin (4 g/dl). After 3 days in media containing bilirubin or heme (at equimolar concentrations), chondrocytes were subsequently incubated in FBS alone for an additional 3 days; only partial reversal of the bilirubin (or heme)-induced inhibition was then observed.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Bilirubin inhibits cartilage metabolism and growth in vitro

Cited by 12 publications

References 15 publications

Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

Responsiveness to phenobarbital in an adult with crigler-najjar disease associated with neurological involvement and skin hyperextensibility

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

Contact Info

Product

Resources

About