Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

Adams, Samuel B.; Setton, Lori A.; Bell, Richard D.; Easley, Mark E.; Huebner, Janet L.; Stäbler, Thomas; Kraus, Virginia B.; Leimer, Elizabeth M.; Olson, Steven A.; Nettles, Dana L.

doi:10.1177/1071100715611176

Cited by 80 publications

(86 citation statements)

References 45 publications

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“…MMP13 is a collagenase with substrate specificity that targets collagen for degradation. Compared to other MMPs, MMP13 has a more restricted expression pattern in connective tissue (53). MMP13 has a much higher catalytic rate than other MMPs for ColII and gelatin, which makes it the most potent peptidolytic enzyme among collagenases (29).…”

Section: Effect Of Mmp13/sox 9/adamts On Articular Chondrocyte Degenementioning

confidence: 99%

Recent advances in the understanding of molecular mechanisms of cartilage degeneration, synovitis and subchondral bone changes in osteoarthritis

Wei

Bai

2016

Connective Tissue Research

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Osteoarthritis (OA), the most common form of degenerative joint disease, is linked to high morbidity. It is predicted to be the single greatest cause of disability in the general population by 2030. The development of disease-modifying therapy for OA currently face great obstacle mainly because the onset and development of the disease involve complex molecular mechanisms. In this review, we will comprehensively summarize biological and pathological mechanisms of three key aspects: degeneration of articular cartilage, synovial immunopathogenesis, and changes in subchondral bone. For each tissue, we will focus on the molecular receptors, cytokines, peptidases, related cell, and signal pathways. Agents that specifically block mechanisms involved in synovial inflammation, degeneration of articular cartilage, and subchondral bone remodeling can potentially be exploited to produce targeted therapy for OA. Such new comprehensive agents will benefit affected patients and bring exciting new hope for the treatment of OA.

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Section: Effect Of Mmp13/sox 9/adamts On Articular Chondrocyte Degenementioning

confidence: 99%

Recent advances in the understanding of molecular mechanisms of cartilage degeneration, synovitis and subchondral bone changes in osteoarthritis

Wei

Bai

2016

Connective Tissue Research

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“…Patients regain range of motion faster and start rehabilitation earlier than after open approaches 5 . Ankle synovial fluid after an intraarticular fracture has significantly elevated inflammatory cytokines and matrix metalloproteinases that may contribute to cartilage damage and synovitis 6 . One additional advantage to arthroscopy is that lavage may theoretically reduce these factors that may be worsening outcomes of otherwise appropriate reductions 7 …”

Section: Discussionmentioning

confidence: 99%

Posterior Malleolus Arthroscopic Reduction and Internal Fixation Technique

Willcox

Johnson

Ryan

2019

Arthroscopy Techniques

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“…One could speculate that a self-adjusting mechanism for IL-10 by an autocrine inhibition takes place in fibro-chondrocytes as well and that this is responsible for less pronounced effects with higher IL-10 dosages. Anyway, the concentration of IL-10 in a healthy joint or even after injury has been found to be lot lower than 10 ng/ml [37, 38]. However, at this moment in time a wide range of thematically related studies, that directly applied IL-10, used dosages ranging prom 10 - 300 ng/ml [22, 39–41].…”

Section: Discussionmentioning

confidence: 99%

IL-10 ameliorates TNF-α induced meniscus degeneration in mature meniscal tissue in vitro

Behrendt¹,

Häfelein

Preusse-Prange

et al. 2017

BMC Musculoskelet Disord

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BackgroundJoint inflammation causes meniscus degeneration and can exacerbate post-traumatic meniscus injuries by extracellular matrix degradation, cellular de-differentiation and cell death. The aim of this study was to examine whether anti-inflammatory interleukin-10 exerts protective effects in an in vitro model of TNF-α-induced meniscus degeneration.MethodsMeniscus tissue was harvested from the knees of adult cows. After 24 h of equilibrium explants were simultaneously treated with bovine TNF-α and IL-10. After an incubation time of 72 h cell death was measured histomorphometrically (nuclear blebbing, NB) and release of glycosaminoglycans (GAG, DMMB assay) and nitric oxide (NO, Griess-reagent) were analysed. Transcription levels (mRNA) of matrix degrading enzymes, collagen type X (COL10A1) and nitric oxide synthetase 2 (NOS2) were measured by quantitative real time PCR. TNF-α-dependent formation of the aggrecanase-specific aggrecan neoepitope NITEGE was visualised by immunostaining. Differences between groups were calculated using a one-way ANOVA with a Bonferroni post hoc test.ResultsAdministration of IL-10 significantly prevented the TNF-α-related cell death (P .001), release of NO (P .003) and NOS2 expression (P .04). Release of GAG fragments (P .001), NITEGE formation and expression of MMP3 (P .007), -13 (P .02) and ADAMTS4 (P .001) were significantly reduced. The TNF-α-dependent increase in COL10A1 expression was also antagonized by IL-10 (P .02).ConclusionIL-10 prevented crucial mechanisms of meniscal degeneration induced by a key cytokine of OA, TNF-α. Administration of IL-10 might improve the biological regeneration and provide a treatment approach in degenerative meniscus injuries and in conditions of post-traumatic sports injuries.

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Inflammatory Cytokines and Matrix Metalloproteinases in the Synovial Fluid After Intra-articular Ankle Fracture

Abstract: In addition to anatomic fracture reduction, these data lend credence to reducing acute intra-articular inflammation through the development of antagonists to these pro-inflammatory and degrading mediators. Likewise, intra-articular lavage might reduce this inflammatory burden.

Cited by 80 publications

References 45 publications

Recent advances in the understanding of molecular mechanisms of cartilage degeneration, synovitis and subchondral bone changes in osteoarthritis

Recent advances in the understanding of molecular mechanisms of cartilage degeneration, synovitis and subchondral bone changes in osteoarthritis

Posterior Malleolus Arthroscopic Reduction and Internal Fixation Technique

IL-10 ameliorates TNF-α induced meniscus degeneration in mature meniscal tissue in vitro

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