Bile salt-dependent lipase in larval turbot, as influenced by density and lipid content of fed prey

Abstract

Turbot larvae were fed three different densities of rotifers (1000, 3000 and 7500 rotifers l 1 ) with a low lipid level (<15% of dry weight) or 7500 rotifers l 1 with a high lipid level (30% of dry weight). The larval consumption of rotifers increased with increasing prey densities and the content of bile salt-dependent lipase (BSDL) in larvae was correlated positively with the ingestion rate from days 6 to 8. This suggests that BSDL synthesis was stimulated by the amount of ingested prey in the early larval phase. However, growth was highest in larvae receiving the medium prey densities, which indicates that the larvae were not able to digest properly the ingested prey at the higher density. No significant effect on the BSDL content was seen in turbot larvae fed rotifers with a high or low lipid content. 2001 The Fisheries Society of the British Isles

Digestive Enzyme Activity during Larval Development of Black Snook, Centropomus nigrescens

“…Two peaks, without statistical significance, were observed at 14 and 37 d.a.h. Similar patterns were reported in other fish species (Oozeki and Baley ; Martínez et al ; Jiménez‐Martínez et al ), although the timing is species specific, depending on the yolk lipid hydrolysis and on the digestive system maturation (Izquierdo et al ; Hoehne‐Reitan et al ). Nevertheless, it can also be possible that these peaks are due to the above‐mentioned overall decrease in digestive activities at 22 d.a.h., possibly related to the change in live feeds administered at 20 d.a.h.…”

“…However, it should also be considered that the increase in whole‐body biomass might be higher in proportion to that of the pancreatic tissue. This might have led to a decrease in BAL content in developing larvae, which was also reported by Hoehne‐Reitan et al (, ) in turbot larvae.…”

Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

“…This may explain what we observed at 11 and 20 DAH, larvae fed high dietary lipid with reduced food intake and lower PL activity. In accordance with this, the bile salt-dependent lipase content in turbot larvae was not significantly affected by the lipid level of the prey but appeared to be a function of the ingestion rate (Hoehne-Reitan et al, 2001). Morais et al (2007) reviewed some recent studies and concluded that neutral lipase synthesis might not be a limiting factor for larval growth.…”

Assessment of digestive enzyme activities during larval development of white bream

“…The major lipase in fishes appears to be a non‐specific and bile salt‐dependent lipase (Gjellesvik et al ., 1992). Its secretion seems to be induced by food (Hoehne‐Reitan et al ., 2001). Oozeki & Bailey (1995) suggested the existence of two types of lipase in marine fish larvae, one related to yolk‐sac absorption (which is rich in phospholipids) and the other, whose activity is developed later, related to digestion of exogenous lipids.…”