Digestive Enzyme Activity during Larval Development of Black Snook, Centropomus nigrescens

Abstract

Black snook, Centropomus nigrescens, have been identified as a promising candidate for aquaculture although, like many of the Centropomid species, high mortality associated with early larval stages presents a significant bottleneck to their commercialization. The digestive capacity of black snook larvae throughout the first 37 d after hatch (d.a.h.) was evaluated by quantifying digestive enzyme activities using biochemical techniques. Results showed that black snook larvae have alkaline proteases at hatching, which are known to be important during the first days of feeding for digestion. Toward the end of the study, acid proteases concentration increased (37 d.a.h.). Enzymes for lipid digestion, pancreatic lipase and bile salt‐activated lipase, were already present in the larvae before exogenous feeding commenced, and their activity increased with age and growth (length). Intracellular digestion, measured as the activity of leucine‐alanine peptidase, was high early on (5 d.a.h.) and decreased as development progressed (next 32 d). In contrast, alkaline phosphatase activity was lowest at first feeding and subsequently increased with age. Overall patterns in enzyme activity suggest the possibility of live feed weaning before 32 d.a.h. if artificial diets can be properly balanced.

Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

“…This lag might be the size of the tank limit the growth of larvae at the end of study, 3-6 L tanks were used in our study rather than (10 m × 10 m × 0.8 m) tanks in Song et al [22] . In the present study, the similar patterns of TL and WW were exhibited in Megalobrama terminalis [17] , Pseudosciaena crocea [23] , Centropomus nigrescens [24] and Odontesthes bonariensis [25] .…”

“…Similar pattern has been documented in Lates calcarifer [3] , Megalobrama terminalis [17] , Centropomus nigrescens [24] , Alosa sapidissima [30] , Sparidentex hasta [33] ,…”

Comparative Study of the Molecular Characterization, Evolution, and Structure Modeling of Digestive Lipase Genes Reveals the Different Evolutionary Selection Between Mammals and Fishes

“…There are two important lipases in animals, one is pancreatic lipase ( pl ) and another is bile salt-activated lipase ( bsal ), which play an important role in hydrolyzing the ester bonds in TAGs during digestive processes ( Karimi et al, 2010 ; Rueda-López et al, 2017 ). Although the reports about these two lipases are abundant in mammals, the main digestive lipase is controversial in fishes ( Rønnestad et al, 2013 ; Anderson et al, 2018 ; Yanes-Roca et al, 2018 ). In this study, we understood the different lipid digestion genes among mammals and fishes, and this is the first report, which comprehends the digestive lipases from whole-genome in the fishes.…”

“…In contrast, in teleost fishes, the most important digestive lipase may be the bile salt-activated lipase ( bsal ). Firstly, it has a certain controversy about the existence of a real pancreatic lipase in fishes ( Rønnestad et al, 2013 ; Anderson et al, 2018 ; Yanes-Roca et al, 2018 ), especially the lipase purified from fishes seemed to require bile salts for activation ( Tocher, 2003 ). Additionally, until now, no reports have confirmed whether the main digestive lipase is pancreatic lipase or bile salt-activated lipase in fish.…”

Effect of feeding strategy on survival, growth, intestine development, and liver status of maraena whitefish Coregonus maraena larvae

“…Diet is closely tied to fish development and has a major influence on welfare and health, a crucial factor in profitable aquaculture. The intestine and liver are directly related to digestion and nutrient utilization (de Alcântara et al, 2019; Yanes‐Roca et al, 2018). A fatty liver indicates wasted calories, as there is little benefit to supplying an energy‐yielding nutrient that is deposited and stored unused in adipose tissue (Hansen et al, 2008).…”