Bile acids combined with fecal sterols: a multiple biomarker approach for deciphering fecal pollution using river sediments

Sánez, Juan; Froehner, Sandro; Hansel, F. A.; Parron, L. M.; Knapik, Heloíse Garcia; Fernandes, Cristóvão Vicente Scapulatempo; Rizzi, Juliane

doi:10.1007/s11368-016-1592-1

Cited by 7 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maximum LCA concentration used here (in terms of carbon) would increase the full-strength seawater DOC concentration (NC-36A) by about 20% and Cypress bog water concentration by about 1% (NC-0A; Table ) and may likely exceed the practical use concentration of nanomaterials in aquatic systems . To date, there have only been limited studies of such bile acids in aquatic systems and those are mostly as an ecological indicator for agricultural input to aquatic ecosystems rather than as an anthropogenic contaminant. , 14 C-radiolabeled nanotubes have been used to measure mineralization of nanotubes, although this work involved metabolism by cultured bacterial isolates rather than natural assemblages . Bile salts as a class of organic matter are reported to be widely metabolized by environmental bacteria .…”

Section: Resultsmentioning

confidence: 99%

“…We investigated activity of a model system of alkaline phosphatase encapsulated in lithocholic acid nanotubes (LCA-AP) after exposure to environmental water samples from five different salinities collected during a Newport River Estuary (NRE) transect from a humic-rich, freshwater Cypress bog (0 practical salinity units (PSU)), through estuarine waters (5, 11, 21 PSU) and ultimately to the coastal Atlantic Ocean (36 PSU). Lithocholic acid (LCA) is a bile acid found in nature from agricultural input and self-assembles in aqueous solutions into a class of carbon-containing nanotubes . The tubular structure confers thermal stability on enzymes encapsulated within nanotubes relative to free enzymes in solution, making it an attractive vector for contaminant-degrading enzymes in environmental applications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Eco-friendly Organic Nanotubes Encapsulating Alkaline Phosphatase and Ecotoxicology of Nanotubes to Natural Bacterial Assemblages in Coastal Estuarine Waters

Montgomery¹,

Collins²,

Boyd³

et al. 2019

ACS Omega

View full text Add to dashboard Cite

Phosphatase-encapsulated nanotubes have potential in environmental remediation of organophosphate contaminants (e.g., pesticides, nerve agents). We investigated alkaline phosphatase (AP) activity when encapsulated in self-assembled lithocholic acid nanotubes (LCA-AP) in water samples along a transect from Cypress bog headwaters through estuarine waters and to Atlantic Ocean seawater. Apparent V max (app V max) for both LCA-AP and unencapsulated AP (Free-AP) was most rapid at mid-estuary and most inhibited at the humic-rich bog. LCA-AP retained a higher-activity percentage, suggesting that encapsulation may afford some protection from denaturing effects of humics. Apparent K m (app K m) of Free-AP (1–2.3 μM) was largely unaffected by preincubation with transect water, whereas app K m of LCA-AP was higher with bog water (5.3 μM) relative to other stations. When comparing Free-AP and LCA-AP, increasing salinity generally decreased the catalytic efficiency of the LCA-AP, but had little effect on that of the Free-AP. In addition, both showed the same pattern of lowest efficiency in bog water, which increased with salinity to 21 practical salinity units before decreasing at full-strength salinity. With the exception of the similarly low values in the bog water (1.04 for LCA-AP, 1.34 for Free-AP), absolute values of catalytic efficiency for LCA-AP were about 17% (range: 14.5–19.3%) of that for Free-AP. Nanotube addition had little ecotoxicological effect on heterotrophic bacterial production in waters sampled along the transect. Microbially associated, intrinsic AP activity showed a similar pattern along the transect to LCA-AP, suggesting that AP environmental control and regulation in nature may inform study of nanomaterials.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eco-friendly Organic Nanotubes Encapsulating Alkaline Phosphatase and Ecotoxicology of Nanotubes to Natural Bacterial Assemblages in Coastal Estuarine Waters

Montgomery¹,

Collins²,

Boyd³

et al. 2019

ACS Omega

View full text Add to dashboard Cite

show abstract

“…With this method, they discovered the bile acids hydrolase activity of Eggerthella lenta and Collinsella aerofaciens ( Wegner et al., 2017 ). Sánez et al applied GC-MS to analysis the quantity of BAs in freshwater sediments ( Sánez et al., 2017 ). More recently, Hu et al established ultrahigh performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) for a sensitive and efficient analysis of BAs in animals with Tripterygium glycoside-induced liver injury, and the results revealed a few BAs differ greatly between control and model groups ( Hu et al., 2020 ).…”

Section: Techniques Utilized In Microbial Analysismentioning

confidence: 99%

The Association Between Intestinal Bacteria and Allergic Diseases—Cause or Consequence?

Han

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The incidence of allergic disorders has been increasing over the past few decades, especially in industrialized countries. Allergies can affect people of any age. The pathogenesis of allergic diseases is complex and involves genetic, epigenetic, and environmental factors, and the response to medication is very variable. For some patients, avoidance is the sole effective therapy, and only when the triggers are identifiable. In recent years, the intestinal microbiota has emerged as a significant contributor to the development of allergic diseases. However, the precise mechanisms related to the effects of the microbiome on the pathogenesis of allergic diseases are unknown. This review summarizes the recent association between allergic disorders and intestinal bacterial dysbiosis, describes the function of gut microbes in allergic disease development from both preclinical and clinical studies, discusses the factors that influence gut microbial diversity and advanced techniques used in microbial analysis. Ultimately, more studies are required to define the host-microbial relationship relevant to allergic disorders and amenable to new therapeutic interventions.

show abstract

“…Furthermore, CFE systems can be freeze-dried in tubes, plates, or on paper-based tickets for extended stability without a cold chain, making them an ideal platform for point-of-using sensing in remote and austere locations . Since the presence of bile acids in water sources can indicate fecal contamination, ,, a CFE sensor could potentially detect fecal contamination in water within a few hours. This would be a significant improvement over traditional approaches with fecal coliform cultures that require greater than 24 h of incubation and further supports the need for a CFE-based bile acid sensor.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, a portable biosensor could be used for at-home testing, which would allow more patients to undergo critical health screenings due to increased affordability and accessibility of the diagnostic assays. Due to their abundance in feces, bile acids have also been used to detect fecal contamination in water and wastewater ways, with DCA measured in livestock pasture runoff at 60 μM concentrations or higher. − Since access to clean drinking water is a major concern for many communities around the globe, having a point-of-use assay to detect bile acids could help communities screen potable water sources for fecal contamination.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor

Beabout

Breedon

Blum

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Bile acids play an important role in digestion and human health, are found throughout the gastrointestinal tract, and are excreted in feces. Therefore, bile acids are promising biomarkers for monitoring health and detecting fecal contamination in water sources.Here, we engineered a bile acid sensor by expressing the transcription factor BreR, a TetR-like repressor from Vibrio cholorae, in Escherichia coli. The sensor was further optimized by screening a promoter library. To further characterize the BreR sensor and increase its utility, we moved expression to a cell-free expression (CFE) system, resulting in an approximately 3 orders of magnitude increase in deoxycholic acid sensitivity. We next optimized this sensor to detect bile acids in fecal water, wastewater, and serum and transferred the CFE sensor to a paper-based assay to enhance fieldability.

show abstract

Bile acids combined with fecal sterols: a multiple biomarker approach for deciphering fecal pollution using river sediments

Cited by 7 publications

References 36 publications

Eco-friendly Organic Nanotubes Encapsulating Alkaline Phosphatase and Ecotoxicology of Nanotubes to Natural Bacterial Assemblages in Coastal Estuarine Waters

Eco-friendly Organic Nanotubes Encapsulating Alkaline Phosphatase and Ecotoxicology of Nanotubes to Natural Bacterial Assemblages in Coastal Estuarine Waters

The Association Between Intestinal Bacteria and Allergic Diseases—Cause or Consequence?

Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor

Contact Info

Product

Resources

About