Bile Acids

Bile acids. XXVI. Metabolism of 12α-hydroxycholanoic acid-24-14C in the rat

“…Since the rat is able to convert cholanoic, lithocholic, chenodeoxycholic, and deoxycholic acids into 618hydroxy acids, the possibility of a 6/3 hydroxylation of 12a-hydroxycholanoic acid was investigated. 6/8,12a-Dihydroxycholanoic acid was prepared by a procedure analogous to that of Ratliff et al (1961) for the preparation of 3a,6/3,12a-trihydroxycholanoic acid from methyl cholate. Support for the structure of the new bile acid was obtained by unambiguous conversion to the known 6/3-hydroxycholanoic acid, and the agreement between the calculated and observed values of molecular rotation, Md, of the new acid, and its derivatives, 6/3-hydroxy-12-ketocholanoic acid and 6/3hydroxycholanoic acid (Table VI).…”

“…Methyl 6/3,12a-Diacetoxy-7 «-bromocholanoate (VII). To a suspension of finely pulverized silver acetate (2.6 g) in 60 ml of glacial acetic acid, 2.5 g of bromine was added slowly with stirring (Ratliff et al, 1961). 408…”