Bifunctional gold nanoparticles as an agglomeration-enhancing tool for highly sensitive lateral flow tests: a case study with procalcitonin

Taranova, Nadezhda A.; Urusov, Alexandr Е.; Садыхов, Э. Г.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1007/s00604-017-2355-4

Cited by 48 publications

(28 citation statements)

References 10 publications

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“…Taranova et al used three labels (functionalized with specific antibody, biotin, and streptavidin, respectively) in a similar format to increase the sensitivity 30-fold in a procalcitonin LFA. 99 Razo et al used two different label types, magnetic nanoparticles conjugated to biotinylated antibodies and streptavidin-coated gold nanoparticles, and a magnetic concentration step to obtain 32-fold sensitivity improvement in an LFA for potato virus X over a conventional LFA without the magnetic concentration or the gold-label-mediated aggregation. 100 Proximity-dependent.…”

Section: Reaction/signalmentioning

confidence: 99%

Sensitivity enhancement in lateral flow assays: a systems perspective

et al. 2019

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Section: Reaction/signalmentioning

confidence: 99%

Sensitivity enhancement in lateral flow assays: a systems perspective

et al. 2019

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“…However, the need to immobilize and store the enzymatic reagents at low temperature may hinder the platform for use in resource-limited environments. Dual AuNP conjugate-based lateral flow assays have also been reported that are based on the surface plasmon resonance effect of the AuNP-antibody conjugates for signal amplification 14 – 16 . However, cumbersome processing and longer incubation times are required to perform these tests.…”

Section: Introductionmentioning

confidence: 99%

Development a stacking pad design for enhancing the sensitivity of lateral flow immunoassay

Tsai

Huang

Chen

et al. 2018

Sci Rep

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Lateral flow immunoassays (LFIAs) have wide application in point-of-care testing, particularly in resource-poor settings. To achieve signal amplification in a gold nanoparticle-based lateral flow assay without an additional procedure or the need for complex fabrication, a new and simple method was developed for using a “stacking pad” configuration that adds an additional membrane between the conjugation pad and test pad to the conventional AuNP-based LFIA format. This design helps to accumulate the antibody and antigen on the stacking pad, hence extending the antigen/antibody binding interactions to enhance the test’s detection sensitivity. With the enhanced lateral flow assay, as low as 1 ng/mL of Protein A and 15.5 ng/mL of C-reactive protein can be visualized with the naked eye. We also successfully applied the stacking pad system in the analysis of C-reactive protein in human serum and synovial fluid samples. These results suggest that this stacking pad LFIA can provide sensitive and on-site prognosis for detection in synovial fluid and serum samples in resource-limited settings.

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“…This difficulty in distinguishing between simultaneously detected targets can be overcome by using different color markers on a single test strip (You et al, ). In particular, the use of multicolor fluorescence nanoparticles cannot only eliminate the cross‐reaction, but also greatly improve the detection limit (Taranova, Urusov, Sadykhov, Zherdev, & Dzantiev, ).…”

Section: Application Scenarios Of Xna Probes In Paper‐based Poctmentioning

confidence: 99%

Paper‐based point‐of‐care test with xeno nucleic acid probes

Xiao

Jin

et al. 2019

Biotech & Bioengineering

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Bridging the unmet need of efficient point-of-care testing (POCT) in biomedical engineering research and practice with the emerging development in artificial synthetic xeno nucleic acids (XNAs), this review summarized the recent development in paperbased POCT using XNAs as sensing probes. Alongside the signal transducing mode and immobilization methods of XNA probes, a detailed evaluation of probe performance was disclosed. With these new aspects, both researchers in synthetic chemistry / biomedical engineering and physicians in clinical practice could gain new insights in designing, manufacturing and choosing suitable reagents and techniques for POCT. K E Y W O R D S aptamer, chemical modification, paper-based analytical devices (PADs), point-of-care testing (POCT), xeno nucleic acids (XNAs)

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Bifunctional gold nanoparticles as an agglomeration-enhancing tool for highly sensitive lateral flow tests: a case study with procalcitonin

Cited by 48 publications

References 10 publications

Sensitivity enhancement in lateral flow assays: a systems perspective

Sensitivity enhancement in lateral flow assays: a systems perspective

Development a stacking pad design for enhancing the sensitivity of lateral flow immunoassay

Paper‐based point‐of‐care test with xeno nucleic acid probes

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