Bifunctional DNA Duplexes Permit Efficient Incorporation of pH Probes into Liposomes

Dolder, Nicolas; Ballmoos, Christoph von

doi:10.1002/cbic.202000146

Cited by 3 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phytosome emulsion with a zeta potential greater than or less than 30 mV is known to be stable. 149 The electrostatic properties of phytosomes can be measured using Doppler velocimetry, 150 zeta sizer, 151 master size, 152 microelectrophoresis, 153 pH-sensitive fluorophores, 154 high-performance capillary electrophoresis, 147 and DLS instruments. 155 Laser Doppler velocimetry is the method for measuring the velocity or linear or vibrational motion of phytosome emulsions using the Doppler Effect in a laser beam.…”

Section: Phytosome Characterizationmentioning

confidence: 99%

Phytosomes as Innovative Delivery Systems for Phytochemicals: A Comprehensive Review of Literature

Barani¹,

Sangiovanni²,

Angarano³

et al. 2021

IJN

107

View full text Add to dashboard Cite

Nowadays, medicinal herbs and their phytochemicals have emerged as a great therapeutic option for many disorders. However, poor bioavailability and selectivity might limit their clinical application. Therefore, bioavailability is considered a notable challenge to improve bio-efficacy in transporting dietary phytochemicals. Different methods have been proposed for generating effective carrier systems to enhance the bioavailability of phytochemicals. Among them, nano-vesicles have been introduced as promising candidates for the delivery of insoluble phytochemicals. Due to the easy preparation of the bilayer vesicles and their adaptability, they have been widely used and approved by the scientific literature. The first part of the review is focused on introducing phytosome technology as well as its applications, with emphasis on principles of formulations and characterization. The second part provides a wide overview of biological activities of commercial and non-commercial phytosomes, divided by systems and related pathologies. These results confirm the greater effectiveness of phytosomes, both in terms of biological activity or reduced dosage, highlighting curcumin and silymarin as the most formulated compounds. Finally, we describe the promising clinical and experimental findings regarding the applications of phytosomes. The conclusion of this study encourages the researchers to transfer their knowledge from laboratories to market, for a further development of these products.

show abstract

Section: Phytosome Characterizationmentioning

confidence: 99%

Phytosomes as Innovative Delivery Systems for Phytochemicals: A Comprehensive Review of Literature

Barani¹,

Sangiovanni²,

Angarano³

et al. 2021

IJN

107

View full text Add to dashboard Cite

show abstract

“…The simple chemistry involved makes this method attractive for many different sensors and the use of DNA hybridization technology ensures a high versatility. 152 In addition to these simple examples, more advanced membrane-anchored sensors rely on structural changes of environmentally sensitive DNA motifs, such as the pH sensitive i-Motif 153,154 , G-quadruplexes 141,155,156 , aptamers 138 , DNAzymes 157 , nanotweezers 158,159 and nanoswitches 160 . In these, the structural changes are transformed into a fluorescent read-out by attaching fluorescence quencher or FRET pairs to the DNA, in order to detect protons, metal ions and even small solutes such as ATP.…”

Section: Rational Design Of Fluorescent Dyes To Follow Enzyme Functionmentioning

confidence: 99%

Current problems and future avenues in proteoliposome research

Amati

Graf

Deutschmann

et al. 2020

Biochemical Society Transactions

Self Cite

View full text Add to dashboard Cite

Membrane proteins (MPs) are the gatekeepers between different biological compartments separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they fulfill a wide variety of cellular functions and their importance is reflected in the increasing number of drugs that target MPs. Functional studies of MPs within a native cellular context, however, is difficult due to the innate complexity of the densely packed membranes. Over the past decades, detergent-based extraction and purification of MPs and their reconstitution into lipid mimetic systems has been a very powerful tool to simplify the experimental system. In this review, we focus on proteoliposomes that have become an indispensable experimental system for enzymes with a vectorial function, including many of the here described energy transducing MPs. We first address long standing questions on the difficulty of successful reconstitution and controlled orientation of MPs into liposomes. A special emphasis is given on coreconstitution of several MPs into the same bilayer. Second, we discuss recent progress in the development of fluorescent dyes that offer sensitive detection with high temporal resolution. Finally, we briefly cover the use of giant unilamellar vesicles for the investigation of complex enzymatic cascades, a very promising experimental tool considering our increasing knowledge of the interplay of different cellular components.

show abstract

“…A phytosome emulsion exhibits stability when its zeta potential exceeds or falls below the threshold of 30 mV. The electrostatic characteristics of phytosomes are assessable through various techniques such as Doppler velocimetry, zeta sizer, master size, microelectrophoresis, pH-sensitive fluorophores, high-performance capillary electrophoresis, and instruments utilizing dynamic light scattering (DLS) [180,[185][186][187][188][189].…”

Section: Zeta Potential (Zp)mentioning

confidence: 99%

The Utilization of Plant-Material-Loaded Vesicular Drug Delivery Systems in the Management of Pulmonary Diseases

Lukhele,

Bassey,

Witika

2023

CIMB

View full text Add to dashboard Cite

Medicinal plants have been utilized to treat a variety of conditions on account of the bioactive properties that they contain. Most bioactive constituents from plants are of limited effectiveness, due to poor solubility, limited permeability, first-pass metabolism, efflux transporters, chemical instability, and food–drug interactions However, when combined with vesicular drug delivery systems (VDDS), herbal medicines can be delivered at a predetermined rate and can exhibit site-specific action. Vesicular drug delivery systems are novel pharmaceutical formulations that make use of vesicles as a means of encapsulating and transporting drugs to various locations within the body; they are a cutting-edge method of medication delivery that combats the drawbacks of conventional drug delivery methods. Drug delivery systems offer promising strategies to overcome the bioavailability limitations of bioactive phytochemicals. By improving their solubility, protecting them from degradation, enabling targeted delivery, and facilitating controlled release, drug delivery systems can enhance the therapeutic efficacy of phytochemicals and unlock their full potential in various health conditions. This review explores and collates the application of plant-based VDDS with the potential to exhibit protective effects against lung function loss in the interest of innovative and effective treatment and management of respiratory illnesses.

show abstract

Bifunctional DNA Duplexes Permit Efficient Incorporation of pH Probes into Liposomes

Cited by 3 publications

References 32 publications

Phytosomes as Innovative Delivery Systems for Phytochemicals: A Comprehensive Review of Literature

Phytosomes as Innovative Delivery Systems for Phytochemicals: A Comprehensive Review of Literature

Current problems and future avenues in proteoliposome research

The Utilization of Plant-Material-Loaded Vesicular Drug Delivery Systems in the Management of Pulmonary Diseases

Contact Info

Product

Resources

About