Bifidobacterium breve MCC1274 with glycosidic activity enhances in vivo isoflavone bioavailability

Abstract

Polyphenols are plant derived compounds that exert many beneficial health effects to the human host. However, associated health benefits of dietary polyphenol are highly dependent on their intestinal metabolism, bioavailability, and absorption. Bifidobacteria, which represent the key members of gut microbiota, have been suggested to promote gut microbial homeostasis and may be involved in the metabolism of polyphenols. In this study, the capabilities of thirteen Bifidobacterium strains in hydrolysing polyphenol glycosides were evaluated. Among the tested strains, Bifidobacterium breve MCC1274 was found to possess the highest β-glucosidase activity and strong capability to convert daidzin and trans-polydatin to their aglycones; while kinetic analysis revealed that B. breve MCC1274 hydrolysed more than 50% of daidzin and trans-polydatin at less than 3 h of incubation. Further investigation using rats with an antibiotics-disturbed microbiome revealed that following the ingestion of daidzin glycoside, oral administration of B. breve MCC1274 significantly enhanced the plasma concentration of daidzein in rats pre-treated with antibiotics as compared to antibiotics-pre-treated control and non-treated control groups. The relative abundance of Actinobacteria and the total numbers of B. breve were also significantly higher in antibiotics-pre-treated rats administered with B. breve MCC1274 than that of the control groups. These findings suggest that B. breve MCC1274 is effective in enhancing the bioavailability of daidzein in the gut under dysbiosis conditions and may potentially improve intestinal absorption of isoflavones and promote human health.

Gut and Vaginal Microbiomes in PCOS: Implications for Women’s Health

“…The glucuronic acid allows estrogens to remain in the body and exert their effects, which plays a vital role in the process. It was reported that β-glucuronidases and β-glucuronides produced by the genera Bifidobacterium ( 38 ), Clostridium, and Lactobacillus, and play roles in the de-conjugation/conjugation of estrogens ( 39 ). The bacteria derived from gut microbiome involve in the metabolism of hormones via producing relative enzymes and thus regulate the circulation of sexual hormones in women.…”

Polydatin, A Glycoside of Resveratrol, Is Better Than Resveratrol in Alleviating Non-alcoholic Fatty Liver Disease in Mice Fed a High-Fructose Diet

“…In the last decade, accumulated evidence suggests gut microbiota, such as Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp, involves in the absorption, metabolism, and bioavailability of polyphenols glycosides ( 7 – 9 ). For instance, glycosides can be hydrolyzed to bioactive aglycones by β-glucosidases that secreted by bacterial in colon and enable the absorption of polyphenols glycosides ( 8 , 9 ). Bifidobacterium strains show the capacity to enhance the bioavailability of daidzein under dysbiosis conditions ( 8 ).…”

“…For instance, glycosides can be hydrolyzed to bioactive aglycones by β-glucosidases that secreted by bacterial in colon and enable the absorption of polyphenols glycosides ( 8 , 9 ). Bifidobacterium strains show the capacity to enhance the bioavailability of daidzein under dysbiosis conditions ( 8 ). In addition, antibiotics treatment caused gut dysbiosis was involved in poor bioconversion of daidzin glycoside and polyphenols ( 8 , 10 ).…”

“…Bifidobacterium strains show the capacity to enhance the bioavailability of daidzein under dysbiosis conditions ( 8 ). In addition, antibiotics treatment caused gut dysbiosis was involved in poor bioconversion of daidzin glycoside and polyphenols ( 8 , 10 ). These reports indicated that gut microbiota-derived β-glucosidases in the colon play a role in improving the absorption and bioavailability of polyphenols glycosides, especially under the condition that a large amount of ingested but unabsorbed polyphenols glycosides can reach and persist to the colon.…”

Ophiopogon Polysaccharide Promotes the In Vitro Metabolism of Ophiopogonins by Human Gut Microbiota

“…Among them, genus Bifidobacterium spp. is one of the most important gut bacteria that releasing all of the four glycosidases selected in the present study, e.g., β-D-glucosidase [35], β-D-xylosidase [36], α-L-rhamnosidase [37] and β-D-fucosidase [38]. Significant difference of the glycosidase activities were observed among the four glycosidases (i.e., β-D-glucosidase > β-D-xylosidase > β-D-fucosidase > α-L-rhamnosidase), these results were consistent with previous studies, and the differences were probably caused by the composition of gut microbiota [19,39,40].…”