Ophiopogon Polysaccharide Promotes the In Vitro Metabolism of Ophiopogonins by Human Gut Microbiota

Wang, Huaiyou; Guo, Shuchen; Peng, Zhi-Tian; Wang, Cheng; Duan, Ran; Dong, Tina Ting Xia; Tsim, Karl Wah-Keung

doi:10.3390/molecules24162886

Cited by 14 publications

(12 citation statements)

References 37 publications

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“…In terms of matrix effect, there was no significant matrix effect observed under current conditions, i.e. 89.6-114.3%, which was within the range of 80-120% [31]. In addition, the RSDs of extraction efficiencies and matrix effects for 3-level QC samples were below 15%, demonstrating a favorable sample preparation.…”

Section: Methods Development and Optimizationmentioning

confidence: 79%

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Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Chin Med

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Background Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods The herbal extract of DBT was fermented with Lactobacillus plantarum. The amounts of flavonoid glucosides and its aglycones in the fermented product were analyzed by using UPLC-MS/MS. In addition, in vitro assays were employed to evaluate the efficacy of the fermented DBT in regulating the activities of α-glucosidase, α-amylase and lipase, as well as their antioxidant capacity (DPPH and T-AOC assays) and anti-glycation property (BSA-methylglyoxal, BSA-fructose, and arginine-methylglyoxal models). Results The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation and anti-glycation. The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion These results suggested that DBT being fermented with the probiotic L. plantarum could pave a new direction for fermentation of herbal extract, as to strengthen its pharmacological properties in providing health benefits.

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Section: Methods Development and Optimizationmentioning

confidence: 79%

“…The assay for β-glycosidase activity was conducted according to the reported method with minor modifications [31]. Briefly, 100 μL fermented sample was acquired by centrifuging at 10,000 rpm for 10 min.…”

Section: Enzymatic Assays β-Glycosidasementioning

confidence: 99%

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Chin Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…In terms of matrix effect, there was no signi cant matrix effect observed under current conditions, i.e. 89.6 -114.3%, which was within the range of 80-120% [31]. In addition, the RSDs of extraction e ciencies and matrix effects for 3-level QC samples were below 15%, demonstrating a favorable sample preparation.…”

Section: Methods Development and Optimizationmentioning

confidence: 82%

“…Enzymatic assays β-glycosidase: The assay for β-glycosidase activity was conducted according to the reported method with minor modi cations [31]. Brie y, 100 μL fermented sample was acquired by centrifuging at 10,000 rpm for 10 min.…”

Section: Uplc-ms/ms Analysismentioning

confidence: 99%

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Preprint

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Background: Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods: The herbal extract of DBT was fermented with Lactobacillus plantarum. The amounts of flavonoid glucosides and its aglycones in the fermented product were analyzed by using UPLC-MS/MS. In addition, in vitro assays were employed to evaluate the efficacy of the fermented DBT in regulating the activities of α-glucosidase, α-amylase and lipase, as well as their antioxidant capacity (DPPH and T-OAC assays) and anti-glycation property (BSA-methylglyoxal, BSA-fructose, and arginine-methylglyoxal models).Results: The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation and anti-glycation. The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion: These results suggested that DBT being fermented with the probiotic L. plantarum could pave a new direction for fermentation of herbal extract, as to strengthen its pharmacological properties in providing health benefits.

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“…Studies have shown that traditional Chinese medicine could obviously modulate the composition of the gut microbiota and the gut microenvironment [8,9,10]. Meanwhile, the gut microbiota is essential for the metabolism of traditional Chinese medicine in vivo [11,12]. Shenling Baizhu San (SLZBS), originated from the Song Dynasty "Taiping Huimin Mixing Agent", is composed by Panax Ginseng, Poria cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandi orus, and Glycyrrhiza uralensis Fisch, which is used for weakened of the spleen and stomach [13].…”

Section: Introductionmentioning

confidence: 99%

ShenLing BaiZhu San alleviates Ulcerative Colitis in Rats by Regulating Gut Microbiota

Gu¹,

Zhou²,

Yao³

et al. 2020

Preprint

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Background: Recent studies have suggested that Shenling Baizhu San (SLBZS), an complementary and alternative medical therapy for ulcerative colitis (UC), alleviate clinic symptoms by the improvement of biochemical criteria and restoration of the intestinal barrier function. SLBZS as a famous Chinese herbal formula has been reportedly used to treat UC, of which mechanism is unknown. This study investigated the therapeutic effects of SLBZS on restoring the gut microbiota in a UC rat model. Methods: We proposed a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced UC rat model to monitor the structural modulation of the gut microbiota. The test period was 10 days (observation for two days after modeling, treatment for 8 days by SLBZS). In this study, the level of inflammatory cytokines and activity of antioxidant enzymes in serum were ascertained by enzyme-linked immunosorbent assay (ELISA) and histological changes of colon were observed. Feces were collected for high-throughput sequencing of 16S rRNA gene. Results: SLBZS partly reduced the diversity of the gut microbiota, while the abundance of that is increased. Furthermore, at the genus level, the relative abundance of short chain fatty acids (SCFA) producing bacteria including Prevotella and Oscillospira increased, while the relative abundance of harmful bacteria including Desulfovibrio, and Bilophila decreased. Additionally, SLBZS could improve the lesions of colon and significantly reduce the expression of Interleukin-6 (IL-6) and Myeloperoxidase (MPO) and increase the activities of Superoxide dismutase (SOD) and Catalase (CAT) in rats serum. Conclusions: These results demonstrate that SLBZS may treat UC effectively by inhibiting inflammation, enhancing antioxidant capacity and regulating gut microbiota.

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Ophiopogon Polysaccharide Promotes the In Vitro Metabolism of Ophiopogonins by Human Gut Microbiota

Cited by 14 publications

References 37 publications

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

ShenLing BaiZhu San alleviates Ulcerative Colitis in Rats by Regulating Gut Microbiota

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