Bifidobacterium breve and Lactobacillus rhamnosus treatment is as effective as budesonide at reducing inflammation in a murine model for chronic asthma

Abstract

BackgroundAsthma is estimated to affect as many as 300 million people worldwide and its incidence and prevalence are rapidly increasing throughout the world, especially in children and within developing countries. Recently, there has been a growing interest in the use of potentially beneficial bacteria for allergic diseases. This study is aimed at exploring the therapeutic effects of long-term treatment with two different beneficial bacterial strains (Bifidobacterium breve M-16 V and Lactobacillus rhamnosus NutRes1) and a glucocorticoid (budesonide), as a reference treatment, on inflammatory response in a murine model for chronic allergic asthma.MethodsTo mimic the chronic disease in asthmatic patients, we used the murine ovalbumin-induced asthma model combined with prolonged allergen exposure. Airway function; pulmonary airway inflammation; airway remodelling, mRNA expression of pattern recognition receptors, Th-specific cytokines and transcription factors in lung tissue; mast cell degranulation; in vitro T cell activation; and expression of Foxp3 in blood Th cells were examined.ResultsLactobacillus rhamnosus reduced lung resistance to a similar extent as budesonide treatment in chronically asthmatic mice. Pulmonary airway inflammation, mast cell degranulation, T cell activation and airway remodelling were suppressed by all treatments. Beneficial bacteria and budesonide differentially modulated the expression of toll-like receptors (TLRs), nod-like receptors (NLRs), cytokines and T cell transcription factors. Bifidobacterium breve induced regulatory T cell responses in the airways by increasing Il10 and Foxp3 transcription in lung tissue as well as systemic by augmenting the mean fluorescence intensity of Foxp3 in blood CD4+ T cells.ConclusionThese findings show that Bifidobacterium breve M-16 V and Lactobacillus rhamnosus NutRes1 have strong anti-inflammatory properties that are comparable to budesonide and therefore may be beneficial in the treatment of chronic asthma.

Regulatory T Cell Depletion Abolishes the Protective Effect of Dietary Galacto-Oligosaccharides on Eosinophilic Airway Inflammation in House Dust Mite–Induced Asthma in Mice

“…Lung cells were washed and resuspended in Roswell Park Memorial Institute 1640 culture medium (Lonza) supplemented with 10% heat-inactivated fetal calf serum and 0.1% penicillin-streptomycin solution (Sigma-Aldrich). Lung cells (4 3 10 5 cells/well) were cultured in medium with or without 50 mg/mL HDM (Greer Laboratories), and supernatant was harvested after 4 d of culture at 37°C in 5% CO 2 and stored at 220°C until further analysis (28).…”

Exploring the role of the microbiota member Bifidobacterium in modulating immune-linked diseases

“…Notably, several studies have reported that different strains of bifidobacteria can modulate T-cell responses in immune-driven diseases. In a murine model for chronic allergic asthma, Bifidobacterium breve M16-V was shown to increase Treg cell responses (defined as CD4 + FoxP3 + cells) and additionally increase the anti-inflammatory cytokine IL-10 in lung tissue [49]. This was also found to have similar effects as budesonide (i.e.…”

“…In an obesity-associated inflammation model, B. pseudocatenulatum CECT 7765 reduced B-cell (CD19 + ) and pro-inflammatory macrophages (F4/80 + CD11c − CD206 + ), as well as increasing Treg responses, which correlated with reduced body weight gain and improved glucose tolerance [66]. Recently, B. breve pre-treatment was shown to significantly decrease the total inflammatory cell number, including decreasing the relative number of eosinophils and neutrophils in a murine airway inflammation model [49].…”

Can Fortified Bifidobacterium with Mycosporin-like Amino Acid be a New Insight for Neurological Disease' s Treatment?

“…The Bifidobacteria are one of the major species of human colon microbiota and they are frequently used as probiotic agents [6]. Bifidobacterium species have immune modulatory, metabolic and anti-inflammatory effects which are not seen ın other gastro intestinal flora species [5,[7][8]. Bifidobacterium species have the highest level of intrinsic hydrogen peroxide resistance causing antioxidant activity.…”