Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties

Speciale, Immacolata; Verma, Ravi; Lorenzo, Flaviana Di; Molinaro, Antonio; Im, Sungbin; Castro, Cristina De

doi:10.1016/j.carbpol.2019.05.006

Cited by 39 publications

(18 citation statements)

References 19 publications

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“…More studies are needed expand the known lexicon of bacterial polysaccharide-immune system interactions, and they may even lead to the discovery of new, bioactive CPS for potential use as therapeutics to improve human health. (13,26,29,30,32,34,41,45,49,53).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Roles of Polysaccharide Capsules in the Intestine

Hsieh

Allen

2020

Front. Immunol.

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The interplay between the immune system and the microbiota in the human intestine dictates states of health vs. disease. Polysaccharide capsules are critical elements of bacteria that protect bacteria against environmental and host factors, including the host immune system. This review summarizes the mechanisms by which polysaccharide capsules from commensal and pathogenic bacteria in the gut microbiota modulate the innate and adaptive immune systems in the intestine. A deeper understanding of the roles of polysaccharide capsules in microbiota-immune interactions will provide a basis to harness their therapeutic potential to advance human health.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory Roles of Polysaccharide Capsules in the Intestine

Hsieh

Allen

2020

Front. Immunol.

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“…Although IRT5, especially B. bifidum , has been studied for its anti-inflammatory effects, the precise mechanism by which IRT5 improves dry eye is not known. 26 , 27 A recent human study reported protein changes in the lacrimal and tear fluid of patients with dry eye. 28 Therefore, to understand the pathophysiologic changes of the lacrimal gland and its role as a possible target organ during crosstalk between the gut microbiome and the immune system, we investigated the mechanism by which IRT5 probiotics alter the gut microbiota and the proteome of the extraorbital lacrimal glands in a mouse model of Sjögren syndrome.…”

mentioning

confidence: 99%

IRT5 Probiotics Changes Immune Modulatory Protein Expression in the Extraorbital Lacrimal Glands of an Autoimmune Dry Eye Mouse Model

Choi

Ryu

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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Purpose While the association between the gut microbiome and the immune system has been studied in autoimmune disorders, little is known about ocular disease. Previously we reported that IRT5, a mixture of five probiotic strains, could suppress autoimmune dry eye. In this study, we investigated the mechanism by which IRT5 performs its immunomodulatory function in a mouse model of autoimmune dry eye. Methods NOD.B10.H2b mice were used as an autoimmune dry eye model. Either IRT5 or PBS was gavaged orally for 3 weeks, with or without 5 days of antibiotic pretreatment. The effects on clinical features, extraorbital lacrimal gland and spleen proteins, and fecal microbiota were analyzed. Results The ocular staining score was lower, and tear secretion was higher, in the IRT5-treated groups than in the PBS-treated groups. After IRT5 treatment, the downregulated lacrimal gland proteins were enriched in the biological processes of defense response and immune system process. The relative abundances of 33 operational taxonomic units were higher, and 53 were lower, in the feces of the IRT5-treated groups than in those of the PBS-treated groups. IRT5 administration without antibiotic pretreatment also showed immunomodulatory functions with increases in the Lactobacillus helveticus group and Lactobacillus hamsteri . Additional proteomic assays revealed a decrease of proteins related to antigen-presenting processes in the CD11b + and CD11c + cells of spleen in the IRT5-treated groups. Conclusions Changes in the gut microbiome after IRT5 treatment improved clinical manifestations in the autoimmune dry eye model via the downregulation of antigen-presenting processes in immune networks.

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“…It can promote the absorption of minerals, produce organic acids such as acetic acid, propionic acid, butyric acid, and lactic acid to stimulate intestinal peristalsis and promote defecation. It can prevent constipation, purify the intestinal environment, decompose carcinogens, stimulate the human immune system, and improve the disease resistance (Gomi et al, 2018 ; Bondue et al, 2019 ; Speciale et al, 2019 ). It is of crucial importance to identify B. bifidum either in food and excreta.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

et al. 2020

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In this work, a quartz crystal microbalance (QCM) sensor has been fabricated using immunoassay for sensitive determination of Bifidobacterium bifidum. Au nanoparticle has been used for amplifying sandwich assays. The proposed immunosensor exhibited a linear detection range between 10 3 and 10 5 CFU/mL with a limit of detection of 2.1 × 10 2 CFU/mL. The proposed immunosensor exhibited good selectivity for B. bifidum sensing with low cross reactivity for other foodborne pathogens such as Lactobacillus acidophilus, Listeria monocytogenes, and Escherichia coli. In addition, the proposed immunosensor has been successfully used for B. bifidum detection in feces samples and food samples. The frequency decreases of 12, 17, and 10 Hz were observed from the milk samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli. The frequency decreases of 8, 15, and 7 Hz were observed from the feces samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli.

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Bifidobacterium bifidum presents on the cell surface a complex mixture of glucans and galactans with different immunological properties

Cited by 39 publications

References 19 publications

Immunomodulatory Roles of Polysaccharide Capsules in the Intestine

Immunomodulatory Roles of Polysaccharide Capsules in the Intestine

IRT5 Probiotics Changes Immune Modulatory Protein Expression in the Extraorbital Lacrimal Glands of an Autoimmune Dry Eye Mouse Model

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

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