Bifidobacteria cell wall-derived exo-polysaccharides, lipoteichoic acids, peptidoglycans, polar lipids and proteins – their chemical structure and biological attributes

Pyclik, Marcelina; Šrůtková, Dagmar; Schwarzer, Martin; Górska, Sabina

doi:10.1016/j.ijbiomac.2019.12.227

Cited by 59 publications

(40 citation statements)

References 170 publications

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“…New concepts of paraprobiotics and postbiotics are intensively studied, paraprobiotics being defined as killed or "ghost" probiotics and postbiotics being any water-soluble compound biosynthesized by probiotics, including Lactic Acid Bacteria (LAB) strains, during fermentations, or any other type of by-products from the bacterial cells after their lysis by physical, chemical, and/or enzymatic agents that possess health-promoting effects to the host [5][6][7][8][9][10]. The living cells are not a necessary condition to exert the beneficial therapeutic effects on consumers.…”

Section: Introductionmentioning

confidence: 99%

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

et al. 2020

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The occurrence of inflammatory responses in humans is frequently associated with food intolerances and is likely to give rise to irritable bowel disease. The use of conventional or unconventional flours to produce gluten-free baking doughs brings important technological and nutritional challenges, and the use of the sourdough biotechnology has the potential to overcome such limitations. In addition, the typical metabolic transformations carried out by Lactic Acid Bacteria (LAB) can become an important biotechnological process for the nutritional fortification and functionalization of sourdoughs due to the resulting postbiotics. In such a context, this research work aimed at isolating and selecting new LAB strains that resort to a wide range of natural environments and food matrices to be ultimately employed as starter cultures in gluten-free sourdough fermentations. Nineteen LAB strains belonging to the genera of Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus were isolated, and the selection criteria encompassed their acidification capacity in fermentations carried out on chickpea, quinoa, and buckwheat flour extracts; the capacity to produce exopolysaccharides (EPS); and the antimicrobial activity against food spoilage molds and bacteria. Moreover, the stability of the LAB metabolites after the fermentation of the gluten-free flour extracts submitted to thermal and acidic treatments was also assessed.

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Section: Introductionmentioning

confidence: 99%

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

et al. 2020

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“…бифидобактерии способны регулировать количество полисахаридов в зависимости от состояния желудочно-кишечного тракта. Полимеры обеспечивают посредничество в межбактериальных взаимодействиях или/и изменяют колонизационные и персистентные свойства бифидофлоры в различных экологических нишах [22,23]. ЭПС могут выступать в качестве микроб-ассоциированных молекулярных паттернов и взаимодействовать с клетками макроорганизма, способствуя формированию биопленок [12,14] [24,25].…”

Section: биологическая роль эпс бифидобактерийunclassified

“…ЭПС, выделенный от В. animalis subsp. lactis RH и от Lactobacillus spp., обладал мощной антиоксидантной активностью [23,30]. ЭПС некоторых штаммов способны снижать уровень холестерина и оказывать антипролиферативное действие in vitro на канцерогенные клетки [2,3,4,19].…”

Section: биологическая роль эпс бифидобактерийunclassified

Bifdobacterial exopolysaccharides: a brief review

Захарова¹,

Леванова²,

Отдушкина³

2021

Fundamentalʹnaâ i kliničeskaâ medicina

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Exopolysaccharide (EPS) production is a widespread phenotypic trait in many commensal and pathogenic microorganisms. In bifidobacteria, the discovery of the eps gene cluster propelled the multiple studies of their EPS, which represent heteropolysaccharides and generally consist of three monosaccharides: D-glucose, D-galactose, and L-rhamnose. EPS of B. animalis subsp. lactis additionally contains mannose while EPS of B. adolescentis and B. longum subsp. longum contains 6-deoxytealose. The number of repeat units in bifidobacterial EPS is a straincharacteristic feature. Precursors of the indicated EPS monomers are glucose-1-phosphate and fructose-6-phosphate, and the synthesis involves nucleotide sugar intermediates. Two molecular systems are implica in polymerisation and polymer secretion in bifidobacteria: ABC transporters and fippase polymerase complex (Wzx/Wzydependent pathway). EPS perform numerous functions. They protect bifidobacteria from aggressive gastrointestinal milieu and reactive oxygen species, provide a scaffold for the bacterial-bacterial interactions, and act as the receptors for phage adsorption. Further, EPS are used by the other members of the gut microbiota as substrates for nutrition, i.e. bifidobacteria regulate the composition and metabolic activity of intestinal microorganisms. Therefore, EPS-producing strains exhibit pronounced antibacterial effects due to the binding of opportunistic and pathogenic microbes. Finally, EPS can act as pathogen-associated molecular patterns. Beneficial effects of bifidobacterial EPS determined the possibility of their use as prebiotics or as a part of symbiotics. The main limitation in this regard is the low yield of the target product when culturing EPS-producing strains. Therefore, current research is aimed at finding novel EPSproducing strains among the bifidobacteria and creating favorable technological conditions that promote EPS production.

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“…One of the main challenges associated with probiotics is the identification and characterization of the molecules and mechanisms associated with its function [ 9 ]. Recently, research has been focused on the characterization of probiotic bacteria-derived postbiotic biomolecules, such as cell-wall peptidoglycans, because they are safer while retaining the beneficial effects on fish host [ 21 ]. The oligosaccharide Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) is a glycan linked to proteins and lipids in prokaryotic and eukaryotic organisms and with potential for the control of infectious diseases [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis

et al. 2021

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Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases.

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Bifidobacteria cell wall-derived exo-polysaccharides, lipoteichoic acids, peptidoglycans, polar lipids and proteins – their chemical structure and biological attributes

Cited by 59 publications

References 170 publications

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

Bifdobacterial exopolysaccharides: a brief review

Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis

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