Bidentate iminodiacetate modified dendrimer for bone imaging

Pes, Lara; Kim, Young; Tung, Ching–Hsuan

doi:10.1016/j.bmcl.2017.01.059

Cited by 6 publications

(2 citation statements)

References 27 publications

(30 reference statements)

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“…2 b) [ 39 ]. Among the five major calcium salts found in human body, HA is the major component and is also found in atherosclerotic plaques and bone neoplasms [ 40 ]. Generally, none of the fluorophores showed strong binding with CPP in the assay.…”

Section: Resultsmentioning

confidence: 99%

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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Background Due to the deep tissue penetration and reduced scattering, NIR-II fluorescence imaging is advantageous over conventional visible and NIR-I fluorescence imaging for the detection of bone growth, metabolism, metastasis, and other bone-related diseases. Methods Bone-targeted heptamethine cyanine fluorophores were synthesized by substituting the meso-carbon with a sulfur atom, resulting in a bathochromic shift and increased fluorescence intensity. The physicochemical, optical, and thermal stability of newly synthesized bone-targeted NIR fluorophores was performed in aqueous solvents. Calcium binding, bone-specific targeting, biodistribution, pharmacokinetics, and 2D and 3D NIR imaging were performed in animal models. Results The newly synthesized S-substituted heptamethine fluorophores demonstrated a high affinity for hydroxyapatite and calcium phosphate, which improved bone-specific targeting with signal-background ratios > 3.5. Particularly, P800SO3-PEG showed minimum nonspecific uptake, and most unbound molecules were excreted into the urinary bladder. Histological analyses demonstrated that P800SO3-PEG remained stable in the bone for over two weeks and was incorporated into bone matrices. Interestingly, the flexible thiol ethylene glycol linker on P800SO3-PEG induced a promising photothermal effect upon NIR laser irradiation, demonstrating potential theranostic imaging. Conclusions P800SO3-PEG shows a high affinity for bone tissues, deeper tissue imaging capabilities, minimum nonspecific uptake in the major organs, and photothermal effect upon laser irradiation, making it optimal for bone-targeted theranostic imaging.

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Section: Resultsmentioning

confidence: 99%

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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“…Osteocalcin is a protein that binds to HA amino acid residues and is produced by osteoblasts during bone formation and healing. Harmatys et al [48] and Pes et al [49] have demonstrated the feasibility of fluorescently-conjugated iminodiacetates for skeletal imaging in metastasized breast cancer models. In vivo studies have shown higher uptake at 19 days with a tetra-iminodiacetate moiety, conjugated to Cy5.5 dye, than the uptake from OsteoSense750 while exhibiting high renal clearance from this moiety within hours of injection [48].…”

Section: Introductionmentioning

confidence: 99%

Changing landscape of optical imaging in skeletal metastases

Cho

Shokeen

2019

Journal of Bone Oncology

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Optical imaging is an emerging strategy for in vitro and in vivo visualization of the molecular mechanisms of cancer over time. An increasing number of optical imaging contrast agents and techniques have been developed in recent years specifically for bone research and skeletal metastases. Visualizing molecular processes in relation to bone remodeling in metastasized cancers provides valuable information for understanding disease mechanisms and monitoring expression of primary molecular targets and therapeutic efficacy. This review is intended to provide an overview of tumor-specific and non-specific contrast agents in the first near-infrared window (NIR-I) window from 650 nm to 950 nm that can be used to study functional and structural aspects of skeletal remodeling of cancer in preclinical animal models. Near-infrared (NIR) optical imaging techniques, specifically NIR spectroscopy and photoacoustic imaging, and their use in skeletal metastases will also be discussed. Perspectives on the promises and challenges facing this exciting field are then given.

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