Bicarbonate uptake in the marine macroalga Ulva sp. is inhibited by classical probes of anion exchange by red blood cells

Drechsler, Zivia; Sharkia, Rajech; Cabantchik, Z. I.; Beer, Sven

doi:10.1007/bf00240893

Cited by 80 publications

(71 citation statements)

References 34 publications

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“…This HCO 3 -transport system was first detailed for the subtropical U. fasciata (Drechsler et al 1993(Drechsler et al , 1994, and it was proposed that the high pH created around thalli of this alga (>10, Beer & Israel 1990) was partly caused by OH -being exchanged outwards in antiport with inward HCO 3 -transport. As a consequence of HCO 3 -transport it was thus visualised that HCO 3 -would dehydrate inside the cells and, while the resulting CO 2 would be fixed by Rubisco, the remaining OH -would be transported outwards and alkalinise the medium.…”

Section: Discussionmentioning

confidence: 99%

“…Björk et al 1992), the presence of a membrane-bound anion transporter (e.g. Drechsler et al 1993), or both (Axelsson et al 1995, Larsson et al 1997. While the direct HCO 3 -uptake system of U. fasciata was ascribed to facilitated transport via arginine groups of an anion exchange protein (Drechsler et al 1994), it was recently shown that HCO 3 -transport in Cladophora glomerata may involve an ATPase (Choo et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Why is Ulva intestinalis the only macroalga inhabiting isolated rockpools along the Swedish Atlantic coast?

Björk¹,

Axelsson²,

Beer³

2004

Mar. Ecol. Prog. Ser.

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Ulva intestinalis (previously called Enteromorpha intestinalis)is the principal marine macroalga growing in isolated rockpools along the Swedish west coast. Given that this alga can take up HCO 3 -under the conditions of high pH and low concentrations of inorganic carbon (Ci) that it creates in those pools, we hypothesized that those conditions would be unfavourable for the photosynthetic performance of other algae such that they would not be able to grow in the same pools. This was tested by transferring 2 of the most common macroalgae, Fucus vesiculosus and Chondrus crispus, from the shallow waters of a bay adjacent to some rockpools, to 2 rockpools containing U. intestinalis. Both photosynthetic electron transport rates (ETR) and maximal quantum yields (F v /F m ) were measured 3 d later. Similar measurements were also performed throughout the day in simulated outdoor rockpools where the 3 species were kept either alone or together for 24 h. It was found that midday ETRs were repressed for all species in the (upper) rockpool as compared to the open bay, but more so for the transferred F. vesiculosus and C. crispus (33 and 0.6%, respectively, of the bay values) than for U. intestinalis (58%). Also, C. crispus showed exceptionally repressed midday F v /F m values in the rockpools (some 22% of that in the bay), and had largely photobleached after 3 d. Similarly, ETRs declined in the simulated rockpools during the day, especially for C. crispus when the other algae were present (to 7% of morning values, compared to 36% when alone). Also, F v /F m declined in the simulated rockpools during the day, indeed much more so for F. vesiculosus and C. crispus when the other algae were present (to ca. 12 and 10% of morning values as compared to 76 and 54%, respectively, when alone). As in the rockpools, the latter alga photobleached when kept together with the other species, but not when alone. Based on these results, and considering their different Ci utilisation mechanisms, it was concluded that the absence in rockpools of algae such as those tested here may be largely due to the adverse conditions of high pH and low Ci concentrations caused by the photosynthetic traits of U. intestinalis. KEY WORDS: Chondrus crispus · Ulva (Enteromorpha) intestinalis · Fucus vesiculosus · Inorganic carbon · Rockpool(s) · pH · PhotosynthesisResale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Why is Ulva intestinalis the only macroalga inhabiting isolated rockpools along the Swedish Atlantic coast?

Björk¹,

Axelsson²,

Beer³

2004

Mar. Ecol. Prog. Ser.

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“…These mechanisms also compensate for the fact that bicarbonate ion (HCO 3 ¡ ) concentrations are higher than dissolved CO 2 concentrations, and therefore involve the enzyme carbonic anhydrase that increases the interconversion of HCO 3 ¡ and CO 2 . Some macroalgae even have the ability to use HCO 3 ¡ directly via anion exchange processes (Drechsler et al 1993;Axelsson et al 1995;Larsson et al 1997). As a result, photosynthesis is very eYcient at ambient DIC conditions, but it is not always saturated, as several macroalgal species have shown increased photosynthetic and growth rates at increased CO 2 concentrations (Gao et al 1991(Gao et al , 1993aKübler et al 1999;Zou 2005).…”

Section: Introductionmentioning

confidence: 99%

Physiological responses of the calcifying rhodophyte, Corallina officinalis (L.), to future CO2 levels

et al. 2011

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Future atmospheric CO 2 levels will most likely have complex consequences for marine organisms, particulary photosynthetic calcifying organisms. Corallina oYcinalis L. is an erect calcifying macroalga found in the inter-and subtidal regions of temperate rocky coastlines and provides important substrate and refugia for marine meiofauna. The main goal of the current study was to determine the physiological responses of C. oYcinalis to increased CO 2 concentrations expected to occur within the next century and beyond. Our results show that growth and production of inorganic material decreased under high CO 2 levels, while carbonic anhydrase activity was stimulated and negatively correlated to algal inorganic content. Photosynthetic eYciency based on oxygen evolution was also negatively aVected by increased CO 2 . The results of this study indicate that C. oYcinalis may become less competitive under future CO 2 levels, which could result in structural changes in future temperate intertidal communities.

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“…Thus the HCO À 3 in seawater can be utilized indirectly as a CO 2 reservoir. Some seaweed species possess a mechanism for direct uptake of HCO À 3 through the plasma membrane, assumed to be facilitated by an anion exchange protein (Drechsler et al, 1993(Drechsler et al, , 1994Axelsson et al, 1995Axelsson et al, , 1999Larsson et al, 1997). The mechanism of HCO À 3 utilization is closely related to the habitat and confers an advantage in natural selection to the algae (Maberly, 1990;Larsson et al, 1997;Mercado et al, 1998;Snoeijs et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Zou

Gao

2010

European Journal of Phycology

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Photosynthetic acquisition of inorganic carbon was studied in the brown seaweed Endarachne binghamiae J. Agardh. Photosynthesis was saturated at 245 mmol photons m À2 s À1 and photoinhibition did not occur at an irradiance as high as 750 mmol photons m À2 s À1 . The dependence of O 2 evolution on inorganic carbon (Ci) concentration demonstrated that the normal Ci composition in natural seawater was not saturating for irradiance-saturated photosynthesis. Three lines of evidence demonstrated that E. binghamiae was able to acquire HCO À 3 as a source of Ci for photosynthesis: (i) the high value of photosynthetic conductance for CO 2 (220.6 mm s À1 ); (ii) the high pH compensation point of 9.7; and (iii) the measured photosynthetic rates being in excess of the theoretical maximum rates supported solely by the CO 2 supply from the spontaneous dehydration of HCO À 3 in the bulk seawater. In order to establish the mechanism of Ci acquisition, specific inhibitors and a proton buffer were applied to examine their inhibitory effects on photosynthesis. No inhibitory effects were found for the proton buffer tris(hydroxymethyl)aminomethane and the anion exchanger inhibitor, 4,4 0 -diisothiocyano-stilbene-2,2 0 -disulphonate. By contrast, photosynthetic O 2 evolution in natural seawater was significantly depressed by the extracellular carbonic anhydrase (CA) activity inhibitor, acetazolamide, and the plasma membrane P-type H þ -ATPase inhibitor, vanadate.These results suggested that carbon acquisition from the natural seawater was mostly through the external CA-mediated HCO À 3 dehydration mechanism, and that P-type H þ -ATPase (proton pump) in the plasma membrane simultaneously functioned in photosynthesis of E. binghamiae. Additional experiments on the O 2 exchange versus pH value relationship indicated that, in contrast to photosynthesis, dark respiration of E. binghamiae was insensitive to the change of pH in the seawater, which resulted in a decreasing instantaneous balance between net carbon gain and respiratory carbon loss at high pH values in seawater.

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Bicarbonate uptake in the marine macroalga Ulva sp. is inhibited by classical probes of anion exchange by red blood cells

Cited by 80 publications

References 34 publications

Why is Ulva intestinalis the only macroalga inhabiting isolated rockpools along the Swedish Atlantic coast?

Why is Ulva intestinalis the only macroalga inhabiting isolated rockpools along the Swedish Atlantic coast?

Physiological responses of the calcifying rhodophyte, Corallina officinalis (L.), to future CO2 levels

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

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