Acquisition of inorganic carbon by<i>Endarachne binghamiae</i>(Scytosiphonales, Phaeophyceae)

Zou, Dinghui; Gao, Kunshan

doi:10.1080/09670260903383909

Cited by 14 publications

(15 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of pH on respiration rates was not significant (Table 3), which is similar to previous seaweed studies (Dromgoole 1978, Zou & Gao 2010. As such, although the experiments were done at pH 7.5, they should reflect the respiratory metabolism of seaweeds adapted to normal seawater pH (8.2).…”

Section: Respiration Ratessupporting

confidence: 86%

“…Ulva has a thinner thallus than the other 2 species, and thus has more active cells per unit of carbon, which is reflected in its higher respiration rates compared to the other species (Fig. 1a,b).The effect of pH on respiration rates was not significant (Table 3), which is similar to previous seaweed studies (Dromgoole 1978, Zou & Gao 2010. As such, although the experiments were done at pH 7.5, they should reflect the respiratory metabolism of seaweeds adapted to normal seawater pH (8.2).…”

supporting

confidence: 84%

See 1 more Smart Citation

Carbon stable isotope discrimination during respiration in three seaweed species

Carvalho¹,

Eyre²

2011

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Very little is known about seaweed stable carbon isotope discrimination during respiration (Δ r , defined here as the difference between respired CO 2 δ 13 C and algal tissue δ 13 C). However, Δ r can give information on carbon metabolic pathways in seaweeds, and can also be helpful to better understand their role in carbon cycling. Here we measured the Δ r of Ulva sp. (Chlorophyta), Pterocladia capillacea Bornet (Rhodophyta) and Sargassum sp. (Ochrophyta) under 3 different experimental conditions: at 15 and 25°C after strong illumination for 30 to 60 min, and at 25°C after at least 20 h in the dark. We observed a correlation between Δ r and the respiratory quotient (RQ, defined as the ratio between CO 2 release and O 2 consumption), suggesting changes in the organic substrate used for respiration in the different treatments. Δ r was positive in most cases, suggesting that carbohydrates were the likely substrate for respiration; however, Δ r (and the RQ) decreased with an increase in temperature, suggesting increased use of lipids as substrates. Although Δ r was not large (averaging 3 ‰), the influence of seaweed respiration on the δ 13 C of dissolved inorganic carbon may need to be taken into account in ecosystems where seaweeds dominate primary production (i.e. eutrophic lagoons). Δ r may also be important when interpreting seaweed δ 13 C values to determine the form of inorganic carbon used in photosynthesis. KEY WORDS:13 C · Fractionation · Macroalgae · Respiration · Respiratory quotient · Short-term incubation Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 437: [41][42][43][44][45][46][47][48][49] 2011 the bulk algal tissue) influence on the overall organism δ 13 C can be important. Aquatic respiration is typically dominated by microbial respiration (Del Giorgio & Williams 2005), including respiration of seaweed detritus. However, respiration by seaweeds comprises the bulk of the respiration in some coastal communities (Middelburg et al. 2005), which means that CO 2 respired by seaweeds may influence the δ 13 C of dissolved inorganic carbon in nearby waters, especially in rocky tidal pools during low tide.In terrestrial plants, there has been a growing recognition that not only Δ p , but also Δ r , can shape plant δ 13 C (Ghashghaie et al. 2003, Pataki 2005, Tcherkez et al. 2010, although this has largely been ignored in aquatic plants. It is un known if the patterns found for Δ r in land plants are also observed in algae; thus, studies addressing Δ r could enhance understanding of the variation of δ 13 C in algae. Investigations in land plants have shown that temperature and light exposure affect Δ r (Tcherkez et al. 2003, Gessler et al. 2009, Werner et al. 2009). An increase in temperature can cause an increased rate of lipid consumption, which is reflected in Δ r (Tcherkez et al. 2003). Light exposure inhibits part of the Krebs cycle, leading to the accumulation of metabolites which are preferentially consumed in the first minutes of darkness,...

show abstract

Section: Respiration Ratessupporting

confidence: 86%

supporting

confidence: 84%

Carbon stable isotope discrimination during respiration in three seaweed species

Carvalho¹,

Eyre²

2011

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…). Other brown seaweeds belonging to other Orders (e.g., Endarachne binghamiae J. Agardh (Scytosiphonales), Zou and Gao , Hizikia fusiformis (Harvey) Okamura, and Sargassum henslowianum C. Agardh (Fucales) Zou et al. , ) are also insensitive to DIDS, and they too rely primarily on the CA ext ‐mediated external HCO 3 − dehydration to fill their internal Ci pool (Larsson and Axelsson , Zou et al.…”

Section: Discussionmentioning

confidence: 99%

“…, ) are also insensitive to DIDS, and they too rely primarily on the CA ext ‐mediated external HCO 3 − dehydration to fill their internal Ci pool (Larsson and Axelsson , Zou et al. , , Zou and Gao ).…”

Section: Discussionmentioning

confidence: 99%

Bicarbonate uptake via an anion exchange protein is the main mechanism of inorganic carbon acquisition by the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae) under variable pH

Fernández

Hurd

Roleda

2014

Journal of Phycology

View full text Add to dashboard Cite

Macrocystis pyrifera is a widely distributed, highly productive, seaweed. It is known to use bicarbonate (HCO3 (-) ) from seawater in photosynthesis and the main mechanism of utilization is attributed to the external catalyzed dehydration of HCO3 (-) by the surface-bound enzyme carbonic anhydrase (CAext ). Here, we examined other putative HCO3 (-) uptake mechanisms in M. pyrifera under pHT 9.00 (HCO3 (-) : CO2 = 940:1) and pHT 7.65 (HCO3 (-) : CO2 = 51:1). Rates of photosynthesis, and internal CA (CAint ) and CAext activity were measured following the application of AZ which inhibits CAext , and DIDS which inhibits a different HCO3 (-) uptake system, via an anion exchange (AE) protein. We found that the main mechanism of HCO3 (-) uptake by M. pyrifera is via an AE protein, regardless of the HCO3 (-) : CO2 ratio, with CAext making little contribution. Inhibiting the AE protein led to a 55%-65% decrease in photosynthetic rates. Inhibiting both the AE protein and CAext at pHT 9.00 led to 80%-100% inhibition of photosynthesis, whereas at pHT 7.65, passive CO2 diffusion supported 33% of photosynthesis. CAint was active at pHT 7.65 and 9.00, and activity was always higher than CAext , because of its role in dehydrating HCO3 (-) to supply CO2 to RuBisCO. Interestingly, the main mechanism of HCO3 (-) uptake in M. pyrifera was different than that in other Laminariales studied (CAext -catalyzed reaction) and we suggest that species-specific knowledge of carbon uptake mechanisms is required in order to elucidate how seaweeds might respond to future changes in HCO3 (-) :CO2 due to ocean acidification.

show abstract

“…This process can be inhibited by the presence of buffers such as Tris (Axelsson et al 2000;Hellblom et al 2001;Moulin et al 2011). On the other hand, some of the species with the highest capacity for HCO 3 -use seem to rely on the presence of a HCO 3 -specific transporter, especially at higher pH (Drechsler et al 1993;Axelsson et al 1999;Zou and Gao 2010) or even under variable pH (Fernández et al 2014. The signature of the 13 C/ 12 C ratio of organic cellular material can be also used to indicate the presence of a CCM in algae.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

García‐Sánchez¹,

Huertas

Fernández³

et al. 2015

Photosynth Res

View full text Add to dashboard Cite

Mechanisms of inorganic carbon assimilation were investigated in the four deep-water kelps inhabiting sea bottoms at the Strait of Gibraltar; these species are distributed at different depths (Saccorhiza polysiches at shallower waters, followed by Laminaria ochroleuca, then Phyllariopsis brevipes and, at the deepest bottoms, Phyllariopsis purpurascens). To elucidate the capacity to use HCO3(-) as a source of inorganic carbon for photosynthesis in the kelps, different experimental approaches were used. Specifically, we measured the irradiance-saturated gross photosynthetic rate versus pH at a constant dissolved inorganic carbon (DIC) concentration of 2 mM, the irradiance-saturated apparent photosynthesis (APS) rate versus DIC, the total and the extracellular carbonic anhydrase (CAext), the observed and the theoretical photosynthetic rates supported by the spontaneous dehydration of HCO3(-) to CO2, and the δ(13)C signature in tissues of the algae. While S. polyschides and L. ochroleuca showed photosynthetic activity at pH 9.5 (around 1.0 µmol O2 m(-2) s(-1)), the activity was close to zero in both species of Phyllariopsis. The APS versus DIC was almost saturated for the DIC values of natural seawater (2 mM) in S. polyschides and L. ochroleuca, but the relationship was linear in P. brevipes and P. purpurascens. The four species showed total and CAext activities but the inhibition of the CAext originated the observed photosynthetic rates at pH 8.0 to be similar to the theoretical rates that could be supported by the spontaneous dehydration of HCO3(-). The isotopic (13)C signatures ranged from -17.40 ± 1.81 to -21.11 ± 1.73 ‰ in the four species. Additionally, the δ(13)C signature was also measured in the deep-water Laminaria rodriguezii growing at 60-80 m, showing even a more negative value of -26.49 ± 1.25 ‰. All these results suggest that the four kelps can use HCO3(-) as external carbon source for photosynthesis mainly by the action of external CAext, but they also suggest that the species inhabiting shallower waters show a higher capacity than the smaller kelps living in deeper waters. In fact, the photosynthesis in the two Phyllariopsis species could be accomplished by the spontaneous dehydration of HCO3(-) to CO2. These differences in the capacity to use HCO3(-) in photosynthesis among species could be important considering the increasing levels of atmospheric CO2 predicted for the near future.

show abstract

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Cited by 14 publications

References 68 publications

Carbon stable isotope discrimination during respiration in three seaweed species

Carbon stable isotope discrimination during respiration in three seaweed species

Bicarbonate uptake via an anion exchange protein is the main mechanism of inorganic carbon acquisition by the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae) under variable pH

Photosynthetic use of inorganic carbon in deep-water kelps from the Strait of Gibraltar

Contact Info

Product

Resources

About