Bicarbonate-induced synthesis of polysaccharide during morphogenesis by synchronous, single-generations of blastocladiella emersonii

Cantino, Edward C.; Goldstein, A.

doi:10.1007/bf00406526

Cited by 18 publications

(5 citation statements)

References 13 publications

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“…Utilization of tech- Incubation with the high concentration of HCOa-necessary to induce RS development causes a considerable decrease in growth rate and a multi tude of chemical and enzymatic changes in B. emersonii. Melanin and 'Y-carotene were reported to be synthesized de novo (28,29); chitin and fat contents were greatly increased (30); and content of a soluble polyglucose elevated (26). Electrophoresis and ion exchange chromatography revealed major changes in protein composition (23,27) and significant changes in the solubility properties and overall base composition of ribonucleic acid were reported to take place (25).…”

Section: Attraction Adhesion and Fusion Of Cellsmentioning

confidence: 96%

Biochemical Aspects of Morphogenesis in Algae and Fungi

Nickerson¹,

Bartnicki-García²

1964

Annu. Rev. Plant. Physiol.

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Section: Attraction Adhesion and Fusion Of Cellsmentioning

confidence: 96%

Biochemical Aspects of Morphogenesis in Algae and Fungi

Nickerson¹,

Bartnicki-García²

1964

Annu. Rev. Plant. Physiol.

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“…Hassid & Abraham, 1957), and its assay with glucose oxidase ('Glucostat' reagent of Worthington Biochem. Corp., Freehold, N.J., U.S.A.) have been described (Cantino & Goldstein, 1961). Methods for its purification have been improved by a slight modification.…”

mentioning

confidence: 99%

Light-Stimulated Polysaccharide and Protein Synthesis by Synchronized, Single Generations of Blastocladiella emersonii

Goldstein

Cantino

1962

Journal of General Microbiology

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SUMMARYImproved methods are described for growing about lo8 to lo9 synchronized single generations of ordinary-colourless (OC) cells of the aquatic fungus Blastocladiella emersonii in submerged liquid 1200 ml. cultures at 24' . The stimulatory effect of white light on the synthesis of the internal pool of soluble protein and soluble polysaccharide, and on the dry wt./cell during ontogeny was assessed. Soluble protein was characterized by fractionation on DEAE-cellulose into a number of components : the quantities/cell were affected differentially by illumination. Light caused a sharp decrease in total glucose-6-phosphate dehydrogenase activity/cell during the last stages of growth, when the light-induced formation of soluble polysaccharide/cell was most pronounced.

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“…RS plants need about 3.5 days to reach maturity under our growth conditions. The rate of exponential growth is similar during RS and OC plant development (4,11). However, RS plants continue the exponential growth for a longer time and reach a larger size than OC plants (1, 4).…”

Section: Resultsmentioning

confidence: 99%

“…The heat-stable isoenzyme is denoted GDH1 and the heat-labile enzyme GDH2. 4. Presence of two NAD-linked GDHs in B. emersonii.…”

Section: Resultsmentioning

confidence: 99%

Variation in levels of enzymes related to energy metabolism in alternative developmental pathways of Blastocladiella emersonii

Ingebretsen¹,

Sanner²

1976

J Bacteriol

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The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two phenotypes, ordinary colorless and resistant sporangia (OC and RS plants), of the water mold Blastocladiella emersonii in synchronized liquid cultures. The OC plants had a generation time of about 12 h, whereas the RS plants required 3.5 days to reach maturity. All the enzymes were present throughout the development of both phenotypes. In zoospores, PFK, FDP, and GDH2 were localized in the cytosol. The IDHNADp activity was distributed with two-thirds in the soluble and one-third in the particulate fraction. GDH, and IDHNAD showed the same distribution and were predominantly present in the particulate fraction, presumably in the mitochondria. Isocitrate lyase was found in the particulate fraction. The enzyme levels changed considerably during development. FDP and IDHNADP varied in a parallel manner. Similarly, the three enzymes PFK, IDHNAD and GDH, showed parallel variations. The activity

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Bicarbonate-induced synthesis of polysaccharide during morphogenesis by synchronous, single-generations of blastocladiella emersonii

Cited by 18 publications

References 13 publications

Biochemical Aspects of Morphogenesis in Algae and Fungi

Biochemical Aspects of Morphogenesis in Algae and Fungi

Light-Stimulated Polysaccharide and Protein Synthesis by Synchronized, Single Generations of Blastocladiella emersonii

Variation in levels of enzymes related to energy metabolism in alternative developmental pathways of Blastocladiella emersonii

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