Light-Stimulated Polysaccharide and Protein Synthesis by Synchronized, Single Generations of Blastocladiella emersonii

Abstract

SUMMARYImproved methods are described for growing about lo8 to lo9 synchronized single generations of ordinary-colourless (OC) cells of the aquatic fungus Blastocladiella emersonii in submerged liquid 1200 ml. cultures at 24' . The stimulatory effect of white light on the synthesis of the internal pool of soluble protein and soluble polysaccharide, and on the dry wt./cell during ontogeny was assessed. Soluble protein was characterized by fractionation on DEAE-cellulose into a number of components : the quantities/cell were affected differentially by illumination. Light caused a sharp decrease in total glucose-6-phosphate dehydrogenase activity/cell during the last stages of growth, when the light-induced formation of soluble polysaccharide/cell was most pronounced.

Metabolism in Penicillium isariiforme on Exposure to Light, with Special Reference to Citric Acid Synthesis

“…W, D. J. GRAAFMANS tion of carbon dioxide in dark-grown cultures is in agreement with the increased accumulation of citric acid Goldstein & Cantino (1962) observed that in synchronized cultures of Blastocladiella emersonii the dry weight, soluble protein and especially the soluble polysaccharide increased more rapidly in the light. They suggested that light inhibited the pathway for glucose degradation, shunting the metabolism towards the manufacture of polysaccharides.…”

Citrate-Induced Citrate Production and Light-Induced Growth of Blastocladiella emersonii

“…In PYG-P medium, light-induced increases (about 90 min.) in generation time were associated with about twofold light-induced increases in cell volume to about 19 x lo4 ,u3 (see Goldstein & Cantino, 1962, for details about this effect). In PYG-PC medium, dark-grown organisms displayed the same generation time and volume as those grown in the light in either PYG-PC or PYG-P media, i.e.…”

“…and 2.5 x 105/ml. ; all other details were as described by Goldstein & Cantino (1962 Lastly, the generation time and the volume at one generation time of organisms grown without CO, supplements in light and darkness on PYG-PC medium were compared with organisms similarly grown on PYG-P medium ( Table 2). In PYG-P medium, light-induced increases (about 90 min.)…”

“…It was later shown (Cantino & Horenstein, 1959) with synchronized single-generation cultures (SSGC) grown on PYG agar media, whereon it was in part reflected in a bicarbonate and/or C 0 2 dependent prolongation of the cell's generation time (GT). Finally, both accelerated growth and the GT effect were achieved (Goldstein & Cantino, 1962) with SSGC in liquid Difco PYG medium supplemented with 5 x M-phosphate (pH 6.7; PYG-P). However, the liquid medium which yielded the best synchronized cultures, i.e.…”

Light-induced Synchronous Conidiation in the Fungus Botrytis cinerea

“…Studies in Biosynthetic Regulation, I 966). Methods for synchronous sporulation have now been developed for Blastocladiella emersonii (Lovett & Cantino, I 960 ;Goldstein & Cantino, 1962;Murphy & Lovett, 1966), Achyla sp. (Griffin & Breuker, 1969), Neurospora crassa (Stine & Clark, 1967;Siege1 et al, 1968), Aspergillus niger (Anderson & Smith, I 971), Penicillium digitaturn (Zeidler & Margalith, 1972) and Schizophyllum commune (Wessels, 1965 ;Schwalb, 1971); synchrony was usually obtained by either nutritional…”