Biased cell migration of fibroblasts exhibiting contact guidance in oriented collagen gels

Dickinson, Richard B.; Guido, Stefano; Tranquillo, Robert T.

doi:10.1007/bf02368241

Cited by 202 publications

(170 citation statements)

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“…2A) (Carter, 1965;Dunn, 1982;Weiss, 1961). Contact guidance has since been demonstrated in vitro for a wide variety of cell types (Dickinson et al, 1994;Dubey et al, 2001;Teixeira et al, 2003;Webb et al, 1995;Wood, 1988). Oriented features of the substrate, such as aligned ECM fibrils, can induce contact-guided behaviours by imposing geometrical constraints on cell-matrix adhesion sites and by providing physical cues to initiate polarisation of cell shape, orientation of cellular organelles and directional cell migration Loesberg et al, 2007;Petrie et al, 2009;Weiss, 1945).…”

Section: Box 1 Front-to-back Polarity In a Migrating Cellmentioning

confidence: 99%

Cell migration: from tissue culture to embryos

2014

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Cell migration is a fundamental process that occurs during embryo development. Classic studies using in vitro culture systems have been instrumental in dissecting the principles of cell motility and highlighting how cells make use of topographical features of the substrate, cell-cell contacts, and chemical and physical environmental signals to direct their locomotion. Here, we review the guidance principles of in vitro cell locomotion and examine how they control directed cell migration in vivo during development. We focus on developmental examples in which individual guidance mechanisms have been clearly dissected, and for which the interactions among guidance cues have been explored. We also discuss how the migratory behaviours elicited by guidance mechanisms generate the stereotypical patterns of migration that shape tissues in the developing embryo.

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Section: Box 1 Front-to-back Polarity In a Migrating Cellmentioning

confidence: 99%

Cell migration: from tissue culture to embryos

2014

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“…Alternatively, cells are placed on top of the lattice for subsequent attachment and penetration of the preformed lattice [61,62]. For the generation of oriented collagen lattices, parallel fiber alignment is achieved by fluid drainage, application of mechanical force (M. Gunzer, P. Friedl, B. Niggemann, E. B. Brö cker, E. Ka9 mpgen and K. S. Za9 nker, submitted), or as a function of an electric or magnetic field and temperature applied during polymerization [63,64]. Matrix architecture and fibril orientation can be visualized at low detail and contrast by phase contrast microscopy [63].…”

Section: -D Collagen Matrix Modelsmentioning

confidence: 99%

The biology of cell locomotion within three-dimensional extracellular matrix

Friedl

Bröcker

2000

Cellular and Molecular Life Sciences (CMLS)

587

463

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Cell migration in three-dimensional (3-D) extracellular matrix (ECM) is not a uniform event but rather comprises a modular spectrum of interdependent biophysical and biochemical cell functions. Haptokinetic cell migration across two-dimensional (2-D) surfaces consists of at least three processes: (i) the protrusion of the leading edge for adhesive cell-substratum interactions is followed by (ii) contraction of the cell body and (iii) detachment of the trailing edge. In cells of flattened morphology migrating slowly across 2-D substrate, contact-dependent clustering of adhesion receptors including integrins results in focal contact and stress fiber formation. While haptokinetic migration is predominantly a function of adhesion and deadhesion events lacking spatial barriers towards the advancing cell body, the biophysics of the tissues require a set of cellular strategies to overcome matrix resistance. Matrix barriers force the cells to adapt their morphology and change shape and/or enzymatically degrade ECM components, either by contact-dependent proteolysis or by protease secretion. In 3-D ECM, in contrast to 2-D substrate, the cell shape is mostly bipolar and the cytoskeletal organization is less stringent, frequently lacking discrete focal contacts and stress fibers. Morphologically large spindle-shaped cells (i.e., fibroblasts, endothelial cells, and many tumor cells) of high integrin expression and strong cytoskeletal contractility utilize integrin-dependent migration strategies that are coupled to the capacity to reorganize ECM. In contrast, a more dynamic ameboid migration type employed by smaller cells expressing low levels of integrins (i.e., T lymphocytes, dendritic cells, some tumor cells) is characterized by largely integrin-independent interaction strategies and flexible morphological adaptation to preformed fiber strands, without structurally changing matrix architecture. In tumor invasion and angiogenesis, migration mechanisms further comprise the migration of entire cell clusters or strands maintaining stringent cell-cell adhesion and communication while migrating. Lastly, cellular interactions, enzyme and cytokine secretion, and tissue remodeling provided by reactive stroma cells (i.e. fibroblasts and macrophages) contribute to cell migration. In conclusion, depending on the cellular composition and tissue context of migration, diverse cellular and molecular migration strategies can be developed by different cell types.

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“…Furthermore, as we will see below, large stresses can align the fibers of the ECM (12)(13)(14). Thus, the migrating cells are subject to contact guidance, i.e., the tendency of cells to move along oriented tissue (15)(16)(17).…”

Section: Introductionmentioning

confidence: 98%

Modeling Contact Guidance and Invasion by Cancer Cells

Sander

2014

Cancer Research

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The first step in the spread of cancer is invasion by malignant cells of the normal tissue surrounding a tumor. There is considerable evidence both in vitro and in vivo that mechanical interactions with the tissue, in particular with the biopolymer network that makes up the extracellular matrix (ECM), are important factors in invasion. The interactions take two forms: (i) contractile cells on the surface of the tumor act on the nearby ECM and remodel it; in some cases, they align the fibers of the biopolymers; (ii) the aligned fibers can enhance invasion via contact guidance, the tendency of motile cells to follow alignment. Here, we give evidence, mainly for in vitro systems, that both effects are important. We discuss how alignment occurs in biopolymers such as collagen-I (a major component of the ECM). We propose a modeling framework for computing alignment and propose phenomenologic models for contact guidance.

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Biased cell migration of fibroblasts exhibiting contact guidance in oriented collagen gels

Cited by 202 publications

References 20 publications

Cell migration: from tissue culture to embryos

Cell migration: from tissue culture to embryos

The biology of cell locomotion within three-dimensional extracellular matrix

Modeling Contact Guidance and Invasion by Cancer Cells

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