Beyond the lateral flow assay: A review of paper-based microfluidics

Carrell, Cody; Kava, Alyssa A.; Nguyen, Michael P.; Menger, Ruth F.; Munshi, Zarina; Call, Zachary; Nussbaum, Mark A.; Henry, Charles S.

doi:10.1016/j.mee.2018.12.002

Cited by 259 publications

(191 citation statements)

References 74 publications

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“…moves by capillary action through various stages were antibodies and conjugated labels (nanoparticles, for instance) can interact and react to the fluid and, finally, show (sandwich assays) or not (competitive assays) a colored line at the test line position. More detailed information about LFAs can be found at (Sajid et al, 2015;Koczula and Gallotta, 2016;Carrell et al, 2019). Figure 3 provides a scheme of biosensor based on LFA where a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label can be seen.…”

Section: Biosensorsmentioning

confidence: 99%

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Campos

Oliveira

Abrantes

et al. 2020

Front. Bioeng. Biotechnol.

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Arboviruses such as yellow fever, dengue, chikungunya and zika are transmitted mainly by the mosquito vector Aedes aegypti. Especially in the tropics, inefficacy of mosquito control causes arboviruses outbreaks every year, affecting the general population with debilitating effects in infected individuals. Several strategies have been tried to control the proliferation of A. aegypti using physical, biological, and chemical control measures. Other methods are currently under research and development, amongst which the use of nanotechnology has attracted a lot of attention of the researchers in relation to the production of more effective repellents and larvicides with less toxicity, and development of rapid sensors for the detection of virus infections. In this review, the utilization of nano-based formulations on control and diagnosis of mosquito-borne diseases were discussed. We also emphasizes the need for future research for broad commercialization of nano-based formulations in world market aiming a positive impact on public health.

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Section: Biosensorsmentioning

confidence: 99%

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Campos

Oliveira

Abrantes

et al. 2020

Front. Bioeng. Biotechnol.

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“…There are many reasons for the disconnect between published works and commercial products, but the most commonly cited argument is poor sensitivity and specicity in mPADs. 14,19 Attempts to improve these criteria are oen successful but result in assays that are far too complicated for their intended use at the pointof-care with untrained end-users. We believe that coupling mPADs with other inexpensive materials could enhance sensitivity and specicity without sacricing usability.…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15][16][17] mPADs are small, portable, inexpensive, easy to dispose of, frequently require no external instrumentation, and can effectively store reagents. 14,16,18,19 Recently, mPADs have been used to detect multiple foodborne pathogens with the goal of creating a simple screening test. [20][21][22][23] In these works, traditional antibody-based immunoassays, enzymatic detection, and electrochemical methods were adapted for use in mPADs.…”

Section: Introductionmentioning

confidence: 99%

Rotary manifold for automating a paper-basedSalmonellaimmunoassay

et al. 2019

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“…Prototypes are incompatible with industrial scale fabrication [1] nor easy to operate by untrained personnel and therefore, not accessible to resource-limited populations. Paper microfluidics [7][8][9] is the only major innovation in recent years to address the need for affordable and simple microfluidic devices to deliver POC health services in the developing world.…”

Section: Introductionmentioning

confidence: 99%

Label-free, High-throughput Assay of Human Dendritic Cells from Whole-blood Samples with Microfluidic Inertial Separation Suitable for Resource-limited Manufacturing

Yousuff¹,

Lim²,

Basha³

et al. 2020

Preprint

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Microfluidics technology has not impacted the delivery and accessibility of point of care health services like diagnosis of infectious disease diagnosis, monitoring health or delivering interventions. Most microfluidics prototypes from academic research are not easy to manufacture with industrial scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives such as labels or reagents. We propose a label- and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths in the range of 200 to 600 µm allows separation of cells into multiple streams according to different size ranges and we utilize a novel technique to collect the closely separated focused cell streams without constricting the channel. When tested on actual human blood cells, 77% of dendritic cells were separated and 80% of cells remained viable after our assay. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations.

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Beyond the lateral flow assay: A review of paper-based microfluidics

Cited by 259 publications

References 74 publications

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Rotary manifold for automating a paper-basedSalmonellaimmunoassay

Label-free, High-throughput Assay of Human Dendritic Cells from Whole-blood Samples with Microfluidic Inertial Separation Suitable for Resource-limited Manufacturing

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