Rotary manifold for automating a paper-based<i>Salmonella</i>immunoassay

Carrell, Cody; Wydallis, Rachel M.; Bontha, Mridula; Boehle, Katherine E.; Beveridge, J. Ross; Geiss, Brian J.; Henry, Charles S.

doi:10.1039/c9ra07106g

Cited by 31 publications

(25 citation statements)

References 53 publications

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“…As of March 2020 we did not find any scientific manuscript describing the development of a LFA using solely Fusion5, instead we found references using Fusion5 as part of LF strips or more complex structures. [172][173][174][175][176][177][178][179] Non-conventional materials. Other interesting low cost alternatives to the conventional LFAs membranes, such as cotton threads, cellulose and glass capillaries have been described in the last years.…”

Section: Assembly Of the Lfamentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Assembly Of the Lfamentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…A 200 mM HCl with 400 mM Bistris (pH = 6.51) solution was used as the LE, while a 10 mM Tricine with 20 mM Bistris (pH = 7.45) solution was used as the TE . CPR was chosen as the analyte, because it is visible to the naked eye, has an adequate mobility and it is produced by β‐galactosidase when chlorophenol red‐β‐D‐galactopyranoside is used as a substrate to detect, for example, bacteria . A 10 µM CPR concentration was used, which is compatible with real applications .…”

Section: Resultsmentioning

confidence: 99%

“…Images were processed using NIH ImageJ . The original image was split into red, green and blue channels and the inverted green channel intensity was measured in the reservoirs and channel sections to quantify the red color of CPR, as previously described . The corrected green channel values (I G ) were obtained subtracting the inverted green channel value of wet paper to the inverted green channel values in the ITP channel sections.…”

Section: Resultsmentioning

confidence: 99%

USB powered microfluidic paper‐based analytical devices

et al. 2019

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Microfluidic paper-based analytical devices (μPADs) allow user-friendly and portable chemical determinations, although they provide limited applicability due to insufficient sensitivity. Several approaches have been proposed to address poor sensitivity in μPADs, but they frequently require bulky equipment for power and/or read-outs. Universal serial buses (USB) are an attractive alternative to less portable power sources and are currently available in many common electronic devices. Here, USB-powered μPADs (USB μPADs) are proposed as a fusion of both technologies to improve performance without adding instrumental complexity. Two ITP USB μPADs were developed, both powered by a 5 V potential provided through standard USB ports. The first device was fabricated using the origami approach. Its operation was analyzed experimentally and numerically, yielding a two-order-of-magnitude sample focusing in 15 min. The second ITP USB μPAD is a novel design, which was numerically prototyped with the aim of handling larger sample volumes. The reservoirs were moved away from the ITP channel and capillary action was used to drive the sample and electrolytes to the separation zone, predicting 25-fold sample focusing in 10 min. USB μPADs are expected to be adopted by minimally-trained personnel in sensitive areas like resource-limited settings, the point-of-care and in emergencies.

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“…Thus, the problem of developing microfluidic platforms for the on-site detection of common foodborne pathogens in milk samples has attracted great interest in the recent literature. Typically, these platforms have been designed for the detection of E. coli [ 86 , 90 , 91 , 92 , 93 ], S. aureus [ 94 , 95 , 96 ], Listeria [ 97 , 98 , 99 ], and Salmonella [ 100 , 101 , 102 , 103 , 104 ].…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

“…Many microfluidic devices have been proposed for the detection of Salmonella in milk samples. Generally speaking, these devices use colorimetric, immunoassay (microchip-based, thread-based, rotary paper-based), EC biosensing, fluorescence, and PCR amplification combined with LIF [ 100 , 101 , 102 , 103 , 104 ]. For example, An et al [ 108 ] presented a microfluidic lab-on-a-chip platform in which a microfluidic droplet device was used to encapsulate single Salmonella cells in a growth medium and the encapsulated droplets were then collected.…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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Rotary manifold for automating a paper-basedSalmonellaimmunoassay

Abstract: Easy-to-use rotary manifold enables an immunomagnetic separation sandwich immunoassay for foodborne pathogen detection at the point-of-need.

Cited by 31 publications

References 53 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

USB powered microfluidic paper‐based analytical devices

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

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