Beyond Serotypes and Virulence-Associated Factors: Detection of Genetic Diversity among O153:H45 CFA/I Heat-Stable Enterotoxigenic
            <i>Escherichia coli</i>
            Strains

Pacheco, Amílcar; Ferreira, Luís Carlos de Souza; Pichel, Mariana; Almeida, Darcy Fontoura de; Binsztein, Norma; Viboud, Gloria I.

doi:10.1128/jcm.39.12.4500-4505.2001

Cited by 16 publications

(24 citation statements)

References 30 publications

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“…Even if they did not belong to the same cluster, it seems likely that they were clonally closely related and probably belonged to the same clonal group. CFA/I-positive strains isolated in different geographical areas also are often closely related (14,16,21). In this study, we found that CFA/I-positive strains isolated from children in India (21) clustered together with our West African CFA/I-positive strains.…”

Section: Discussionsupporting

confidence: 48%

“…Serotyping is a commonly used method for indicating clonal relatedness between bacteria, including ETEC. Although there are indications that ETEC strains that share the same serotype often are clonally closely related (14,15,16,21), it seems likely that clonally related strains may also have different serotypes, similar to what has been found for Shigella strains (19).…”

Section: Discussionmentioning

confidence: 87%

“…Earlier fingerprint analyses showed that ETEC strains sharing the same toxin-CF profile are often clonally closely related, even when the strains are isolated in different geographical areas (14,15,16,21). These data indicate that some ETEC strains belong to widespread clonal groups, where a clonal group is defined to comprise all of the descendants of the ancestral strain the first possessed their shared virulence genes.…”

Section: Discussionmentioning

confidence: 99%

“…However, Steinsland et al previously found that natural ETEC infections protect young children against new infections with ETEC strains that have the same toxin-CF profile but that the CFs do not seem to contribute measurably to this protection (22). Earlier studies showed that ETEC strains that have the same toxin-CF profile are often clonally closely related (14,15,16,21).…”

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confidence: 99%

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Clonal Relatedness of Enterotoxigenic Escherichia coli Strains Isolated from a Cohort of Young Children in Guinea-Bissau

et al. 2004

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In a cohort study of 200 young children in Guinea-Bissau, it was previously found that some enterotoxigenic Escherichia coli (ETEC) strains were more pathogenic than others, depending on the type of toxin that they could produce, and that natural ETEC infections induced substantial protection against new infections with ETEC strains that had the same combination of toxins and colonization factors (CFs). We wanted to describe the clonal relatedness of the ETEC strains isolated during this study and to investigate whether the protective antigens and the virulence factors that were responsible for the pathogenic traits were common to strains that were clonally closely related or whether they were more likely to be encoded by the ETEC virulence plasmids that normally encode the toxins and the CFs. By performing repetitive sequence-based PCR analysis of strains representing 452 infections, we found that strains that had the same toxin-CF profile were usually closely related, although a few were unrelated. Strains that did not have the same toxin-CF profiles but that were positive for a given toxin or for a given CF were not consistently more closely related to each other than to strains that were negative for the same toxin or CF. Our results indicate that the pathogenic traits of ETEC were mainly attributed to genes carried on the ETEC virulence plasmids. Because most strains that had the same toxin-CF profile were closely related, it seems likely that the toxin-CF-specific protection was clonal and was not targeting antigens encoded by the virulence plasmids. These results are of relevance to the ETEC vaccine development effort.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Clonal Relatedness of Enterotoxigenic Escherichia coli Strains Isolated from a Cohort of Young Children in Guinea-Bissau

et al. 2004

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“…Macrorestriction profiling of genomic DNA was performed with the restriction endonuclease XbaI, as described previously (24). DNA was resolved by PFGE with a CHEF-DRII apparatus (Bio-Rad Laboratories, Richmond, CA).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Analysis of Enterotoxigenic Escherichia coli in Samples Obtained from Egyptian Children Presenting to Referral Hospitals

et al. 2009

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Hospital surveillance was established in the Nile River Delta to increase the understanding of the epidemiology of diarrheal disease among Egyptian children. Between September 2000 and August 2003, samples obtained from children less than 5 years of age who had diarrhea and who were seeking hospital care were cultured for enteric bacteria. Colonies from each culture with a morphology typical of that of Escherichia coli were tested for the heat-labile (LT) and heat-stable (ST) toxins by a GM-1-specific enzyme-linked immunosorbent assay and colonization factor (CF) antigens by an immunodot blot assay. Enterotoxigenic E. coli (ETEC) isolates were recovered from 320/1,540 (20.7%) children, and ETEC isolates expressing a known CF were identified in 151/320 (47%) samples. ST CFA/I, ST CS6, ST CS14, and LT and ST CS5 plus CS6 represented 75% of the CFs expressed by ETEC isolates expressing a detectable CF. Year-to-year variability in the proportion of ETEC isolates that expressed a detectable CF was observed (e.g., the proportion that expressed CFA/I ranged from 10% in year 1 to 21% in year 3); however, the relative proportions of ETEC isolates expressing a CF were similar over the reporting period. The proportion of CF-positive ETEC isolates was higher among isolates that expressed ST. ETEC isolates expressing CS6 were isolated significantly less often (P < 0.001) than isolates expressing CFA/I in children less than 1 year of age. Macrorestriction profiling of CFA/I-expressing ETEC isolates by using the restriction enzyme XbaI and pulsed-field gel electrophoresis demonstrated a wide genetic diversity among the isolates that did not directly correlate with the virulence of the pathogen. The genome plasticity demonstrated in the ETEC isolates collected in this work suggests an additional challenge to the development of a globally effective vaccine for ETEC.

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Unexpected finding of the "tropical" bacterial pathogen Plesiomonas shigelloides from lake water north of the Polar Circle

González-Rey¹,

Svenson²,

Eriksson

et al. 2003

Polar Biology

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Beyond Serotypes and Virulence-Associated Factors: Detection of Genetic Diversity among O153:H45 CFA/I Heat-Stable Enterotoxigenic Escherichia coli Strains

Cited by 16 publications

References 30 publications

Clonal Relatedness of Enterotoxigenic Escherichia coli Strains Isolated from a Cohort of Young Children in Guinea-Bissau

Clonal Relatedness of Enterotoxigenic Escherichia coli Strains Isolated from a Cohort of Young Children in Guinea-Bissau

Phenotypic and Genotypic Analysis of Enterotoxigenic Escherichia coli in Samples Obtained from Egyptian Children Presenting to Referral Hospitals

Unexpected finding of the "tropical" bacterial pathogen Plesiomonas shigelloides from lake water north of the Polar Circle

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