Unexpected finding of the "tropical" bacterial pathogen Plesiomonas shigelloides from lake water north of the Polar Circle

González-Rey, Carlos; Svenson, Stefan B.; Eriksson, Laila; Ciznár, I; Krovacek, Karel

doi:10.1007/s00300-003-0521-0

Cited by 18 publications

(15 citation statements)

References 19 publications

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“…Enteric P. shigelloides infections in Finnish patients have been documented (Rautelin et al 1995), and in the same country, this microorganism has been recovered from various animal species (Niskanen and Salmela 2000). We have isolated and characterized these bacteria from lakes and river water in the central part of Sweden and even from a lake above the Polar Circle (González-Rey et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent

et al. 2011

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The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic.

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Section: Discussionmentioning

confidence: 99%

Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent

et al. 2011

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“…One early study described a psychrophilic strain of P. (Aeromonas) shigelloides (CDC882-69) with a minimal growth temperature of 0°C, but this seems to be an aberrant finding for the group as a whole (36). Few if any strains routinely grow below 10°C (35,37), although a number of Plesiomonas isolates were recovered from Lake Vettasjärvi in the Arctic region of Sweden, which is north of the Polar Circle (38). The recorded water temperature there was 9°C with a pH of 6.5.…”

Section: Environmental Distribution and Ecologic Associationsmentioning

confidence: 99%

Plesiomonas shigelloides Revisited

Janda¹,

2016

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SUMMARYAfter many years in the familyVibrionaceae, the genusPlesiomonas, represented by a single species,P. shigelloides, currently resides in the familyEnterobacteriaceae, although its most appropriate phylogenetic position may yet to be determined. Common environmental reservoirs for plesiomonads include freshwater ecosystems and estuaries and inhabitants of these aquatic environs. Long suspected as being an etiologic agent of bacterial gastroenteritis, convincing evidence supporting this conclusion has accumulated over the past 2 decades in the form of a series of foodborne outbreaks solely or partially attributable toP. shigelloides. The prevalence ofP. shigelloidesenteritis varies considerably, with higher rates reported from Southeast Asia and Africa and lower numbers from North America and Europe. Reasons for these differences may include hygiene conditions, dietary habits, regional occupations, or other unknown factors. Other human illnesses caused byP. shigelloidesinclude septicemia and central nervous system disease, eye infections, and a variety of miscellaneous ailments. For years, recognizable virulence factors potentially associated withP. shigelloidespathogenicity were lacking; however, several good candidates now have been reported, including a cytotoxic hemolysin, iron acquisition systems, and lipopolysaccharide. WhileP. shigelloidesis easy to identify biochemically, it is often overlooked in stool samples due to its smaller colony size or relatively low prevalence in gastrointestinal samples. However, one FDA-approved PCR-based culture-independent diagnostic test system to detect multiple enteropathogens (FilmArray) includesP. shigelloideson its panel. Plesiomonads produce β-lactamases but are typically susceptible to many first-line antimicrobial agents, including quinolones and carbapenems.

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“…On occasion, RAPD and PFGE have been found to be superior to ribotyping, multilocus enzyme electrophoresis, and restriction enzyme analysis (González-Rey, 2003). RAPD can therefore be considered a promising, simple, rapid and reliable sub-species typing technique.…”

Section: Introductionmentioning

confidence: 96%

“…RAPD can therefore be considered a promising, simple, rapid and reliable sub-species typing technique. Such techniques already have been used on P. shigelloides in a study of the isolation of this pathogen from lake water north of the Polar Circle in Sweden (González-Rey et al, 2003). The issue of the reproducibility and usefulness of RAPD profiling has been widely discussed by many authors in the study of other pathogens (Boerlin et al, 1995;Martinez et al, 2003;Maurer et al, 1998).…”

Section: Introductionmentioning

confidence: 98%

Genetic Variability Among Isolates ofPlesiomonas shigelloidesfrom Fish, Human Clinical Sources and Fresh Water, Determined by RAPD Typing

González-Rey²,

Krovacek³

et al. 2006

Food Biotechnology

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Plesiomonas shigelloides has been a suspected pathogen for the past 40 years. Random amplified polymorphic DNA (RAPD) analysis with two random primers was used to examine the genetic diversity among 26 strains of P. shigelloides: 10 from fresh water, 6 from fish, and 10 from human clinical sources. There was notable genetic variability among most of the isolates, and none of the isolates had the same composite RAPD profile. Our results indicate that most of the isolates from the same source grouped together and that the isolates from fish had a closer linkage to the human clinical isolates than did the freshwater isolates, suggesting that fish may be the more serious source for potential risk of infection. We also found that certain human clinical isolates had almost the same RAPD profiles as certain of the isolates from freshwater and fish, indicating that P. shigelloides from both sea food and freshwater should be considered potential pathogens.

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Unexpected finding of the "tropical" bacterial pathogen Plesiomonas shigelloides from lake water north of the Polar Circle

Cited by 18 publications

References 19 publications

Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent

Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent

Plesiomonas shigelloides Revisited

Genetic Variability Among Isolates ofPlesiomonas shigelloidesfrom Fish, Human Clinical Sources and Fresh Water, Determined by RAPD Typing

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