.BETA.-tricalcium phosphate and basic fibroblast growth factor combination enhances periodontal regeneration in intrabony defects in dogs

Abstract

The combined effect of β-tricalcium phosphate and basic fibroblast growth factor was observed in the regeneration of periodontal tissue in dogs. For this purpose, either β-TCP and FGF-2 (β-TCP/FGF-2 group) or FGF-2 alone (FGF-2 group) was applied in intrabony defects. The control group received β-TCP alone. The tissues were histologically examined at 2, 4, or 8 weeks following treatment. The control group was characterized by incomplete, newly formed bone around β-TCP particles. The β-TCP/FGF-2 group showed a statistically significant increase in both new bone and cementum formation compared to the FGF-2-alone group (76.3% vs. 65.3%, p<0.01; 81.0% vs. 68.3%, p<0.01, respectively). These findings suggested that β-TCP may be a suitable scaffold for FGF-2 and that the combination of β-TCP and FGF-2 can enhance bone and cementum formation.

Periodontal regeneration following application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs

“…The different application methods of bFGF-2 may explain the different types of epithelial repair in this study. These results are similar to the findings in one-wall defects and two-wall defects in dogs following application of the combination of bFGF-2 and β-TCP 15,16) . However, the effect of bFGF-2 on gingival epithelial cells in vivo remains unclear.…”

“…(new cementum formation, 80%), who reported similar observations in one-wall defects and two-wall defects in dogs following application of bFGF-2 in conjunction with β-TCP 15,16) . The findings of the present experiment demonstrated that the combination of bFGF-2 and β-TCP failed to interfere with new cementum formation by bFGF-2.…”

“…We attempted to determine the optimal dose of bFGF-2 for periodontal regeneration in dogs from several previous reports. bFGF-2 had a dose-dependent effect on periodontal regeneration at a dose ranging from 0 to 0.16% per implant [3][4][5][6][7][8][9][10][11][12][13][14][15][26][27][28][29] . Hayashi et al reported that new bone formation was smaller in the 0.56% bFGF-2 (100 µg bFGF-2/3×3×2 mm gelatin hydrogel) implant compared to the 0.056% bFGF-2 (10 µg bFGF-2/3×3×2 mm gelatin hydrogel) implant; however, there were no significant differences between them 29) .…”

“…The resorption of β-TCP in vivo involves chemical dissolution 33,34) and phagocytosis by osteoclasts 15,17) . β-TCP remained in 22.4±6.3% of the furcation area in the TF group.…”

“…Several studies have reported that bFGF-2 induces potent periodontal tissue regeneration along with new alveolar bone and cementum formation without aberrant healing such as root resorption and ankylosis in critical size defects of animal models [12][13][14] . Some studies reported that the combination of bFGF-2 and granular betatricalcium phosphate (β-TCP) implanted in 1-wall and 2-wall periodontal defects resulted in good periodontal regeneration in dogs 15,16) .…”