.BETA.-Naphthoflavone Caused Up-Regulation of AhR Regulated GFP in Transgenic Zebrafish

Seok, Seung Hyeok; Park, Duk Woong; Park, Jong Hwan; Cho, Sun A.; Baek, Min Won; Lee, Hui Young; Kim, Dong Jae; Jin, Bo Hwan; Ryu, Doug-Young; Park, Jae Hak

doi:10.1538/expanim.53.479

Cited by 4 publications

(2 citation statements)

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“…Because CYP1A1 monooxygenase activity depends on AhR activation, we used the CYP1A1 promoter/enhancer region to design an AhR-responsive reporter construct. Previously, our study indicated that the 5' regulatory region is sufficient for transcriptional activation of CYP1A1 by AhR [ 26 ]. Before injecting zebrafish eggs with an EGFP reporter construct under regulatory control of the human CYP1A1 promoter, we had to determine whether this 5' regulatory region of CYP1A1 was responsive to AhR ligands in zebrafish.…”

Section: Resultsmentioning

confidence: 99%

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In vivo alternative testing with zebrafish in ecotoxicology

et al. 2008

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Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhRE-EGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3',4,4',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.

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Section: Resultsmentioning

confidence: 99%

“…The human AhR-regulated reporter plasmid, phAhRE-EGFP, was described in detail in our previous study [ 26 ]. In brief, it was constructed by fusing a portion of the two consensus aryl hydrocarbon response element (AhRE) sequences, and this oligonucleotide was ligated into pEGFP vector using the Ase I and Hin d III sites ( Fig.…”

Section: Methodsmentioning

confidence: 99%