Quantitative GFP fluorescence as an indicator of arsenite developmental toxicity in mosaic heat shock protein 70 transgenic zebrafish

The zebrafish embryo model in environmental risk assessment—applications beyond acute toxicity testing

“…Transgenic models, i.e. zebrafish strains that express GFP controlled by the regulatory sequence of a toxicant-sensitive gene may assist in this analysis of toxicant-sensitive genes and provide simple readout systems (see Blechinger et al 2007;Hill et al 2003;Mattingly et al 2001;Seok et al 2007;Yu et al 2006 for available transgenic strains that monitor the expression of ahr, hsp70, shh and neurogenin).…”

Developmental toxicity screening in zebrafish

“…The two most commonly assessed effects are pericardial edema and changed heart rates. Pericardial edema was evident in embryos exposed to TCDD (Henry et al, 1997;Belair et al, 2001;Teraoka et al, 2002Teraoka et al, , 2003Prasch et al, 2003;Bello et al, 2004;Antkiewicz et al, 2005;Carney et al, 2006;Ton et al, 2006), ethanol (Reimers et al, 2004;Hallare et al, 2006;Matsui et al, 2006;Kashyap et al, 2007;Sylvain et al, 2010), and heavy metals, such as cadmium (Cheng et al, 2000(Cheng et al, , 2001Hallare et al, 2005), arsenic (in the form of sodium arsenite) (Seok et al, 2007b), and methyl mercury (Yang et al, 2010) (Table 5). Other compounds that caused pericardial edema included industrial products, such as the plasticizer bisphenol A (BPA) (Duan et al, 2008), flame retardant tetrabromoBPA (Kuiper et al, 2007), or the pesticides dichlorvos (Sisman, 2010) and pentachlorophenol (Duan et al, 2008) (Table 5).…”

“…The first cranial cartilage is apparent at 2 dpf (Yelick and Schilling, 2002). Again, characterization of toxicological effects on zebrafish focuses on morphological abnormalities observable in a light microscope, such as spinal lordosis (curvature of the spine) resulting from treatments such as benomyl ) and arsenite (Seok et al, 2007b), and cranial malformations from exposure to cadmium (Cheng et al, 2001), PCB126 (Grimes et al, 2008), and TBBPA (Kuiper et al, 2007) (Tables 2 and 6). However, skeletal endpoints are assessed relatively late in development and early markers for skeletal effects would be helpful.…”

Transgenic Zebrafish Biosensor for the Detection of Cadmium and Zinc Toxicity

“…Reporter expression in the transgenic fish increased with increasing concentration of cadmium and zinc suggesting dose dependency ( Figure 3). Several researchers reported similar dose dependent pattern in case of human and zebrafish hsp70 promoters in detecting Cd 2+ (0.2 to 125 M), As 3+ (10 to 300 M) and CuSO 4 (1 to 1.5 M) 16,17,29 . For reporter gene expression analysis, the zebrafish larvae were sampled from the experimental groups as described for fluorescence imaging and stored in RNA later solution (Qiagen, NL) at -20C.…”

“…Several transgenic zebrafish biosensors employing estrogen response elements (ERE), aryl hydrocarbon response elements (AHREs), heat-shock protein promoter elements (HSPs), DNA-damage inducible promoter elements, etc. are reported for monitoring aquatic pollution [14][15][16][17][18][19][20] . Metallothioneins (MTs) are small, ubiquitous, cysteine-rich proteins that are triggered in response to the toxic levels of heavy metals and hence are regarded as effective markers of metal pollution in aquatic systems 21 .…”