Beta‐catenin causes fibrotic changes in the extracellular matrix via upregulation of collagen I transcription

Roh, Mi Ryung; Kumar, Raj; Rajadurai, Anpuchchelvi; Njauw, Ching‐Ni Jenny; Ryoo, U.H.; Chung, Kee Yang

doi:10.1111/bjd.15079

Cited by 13 publications

(11 citation statements)

References 10 publications

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“…Studies had already shown that miR-574-5p was related to the proliferation and migration of various tumor cells and the differentiation of fibroblasts. 28,[34][35][36][37] The downstream gene of miR-574-5p was predicted to be SMAD4 SMAD4 is a transcriptional activator. It is also and an important molecule in the TGF-ß signaling pathway, plays a key role in the transfer of TGF-ß signals from cell surface to the nucleus.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, we found that hsa_circ_0001320 was interacted with miR‐574‐5p. Studies had already shown that miR‐574‐5p was related to the proliferation and migration of various tumor cells and the differentiation of fibroblasts 28,34‐37 . The downstream gene of miR‐574‐5p was predicted to be SMAD4 SMAD4 is a transcriptional activator.…”

Section: Discussionmentioning

confidence: 99%

“…The upregulation of two circRNAs (hsa_circ_0001320 and circCOL5A1) was consistent with sequencing data ( P < .05). To explore the heterogeneity of keloids and the relationship of different regions of keloids in the pathogenesis, 28 we also compared these two circRNAs between keloids and the normal skin tissues around them, as well as and normal skin of the people without keloids. Meanwhile in order to understand whether the expression level of the two circRNAs were differences between the HT and LT areas, we also compared separately.…”

Section: Discussionmentioning

confidence: 99%

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Identification of differentially expressed circular RNAs in keloid and normal skin tissue by high‐throughput sequencing

Zhang

Liu

et al. 2021

Dermatologic Therapy

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Keloid is a kind of pathological skin scar with unclear molecular pathology. Circular RNAs (circRNAs) are involved in the occurrence and development of many diseases; however, their relationship with keloid is not well understood. To investigate the involvement of dysregulated circRNAs in keloid. Thirty-seven keloids and 37 normal skin tissues were collected, and the changes of circRNAs, microRNAs (miRNAs) and mRNAs in 3 keloids and 3 normal samples by high-throughput sequencing were detected first. Based on the circRNA-miRNA-mRNA interaction network construction, gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis combining several signaling pathways associated with keloid formation and progression, the circRNAs required further verification were screened out. The expression levels of the selected circRNAs were verified in 37 keloids and 37 normal skin tissues using quantitative real-time polymerase chain reaction (QPCR). The interaction of candidate circRNA and its predicted binding miRNA was tested by dualluciferase reporter gene experiment. Compared with normal controls, there was an average of 120 and 12 circRNAs, 44 and 63 miRNAs, 656 and 156 mRNAs were upregulated and downregulated, respectively, in keloids. According to the analysis of bioinformation, six circRNAs were picked out. The QPCR validation results of two upregulated circRNAs (hsa_circ_0001320 and circCOL5A1) were consistent with previous sequencing results. The interaction between hsa_circ_0001320 and miR-574-5p was confirmed. This study makes it clear that the abnormal expression of cir-cRNAs may be related to the pathological process of keloid.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of differentially expressed circular RNAs in keloid and normal skin tissue by high‐throughput sequencing

Zhang

Liu

et al. 2021

Dermatologic Therapy

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“…83 Betacatenin 1 has been found to be involved in tissue fibrosis and regulation of COL1A1/A2 synthesis. 84 In addition, betacatenin 1 is essential for full thickness skin wound healing in mice through the regulation of motility and adhesion of macrophage cells. 85 Moreover, it has been demonstrated that there is an increased level of beta-catenin 1 in hypertrophic scar and keloid tissues compared with atrophic scar tissue.…”

Section: ■ Discussionmentioning

confidence: 99%

Characterization of the Blister Fluid Proteome for Pediatric Burn Classification

et al. 2018

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Blister fluid (BF) is a novel and viable research matrix for burn injury study, which can reflect both systemic and local microenvironmental responses. The protein abundance in BF from different burn severities were initially observed using a 2D SDS-PAGE approach. Subsequently, a quantitative data independent acquisition (DIA) method, SWATH, was employed to characterize the proteome of pediatric burn blister fluid. More than 600 proteins were quantitatively profiled in 87 BF samples from different pediatric burn patients. These data were correlated with clinically assessed burn depth and time until complete wound re-epithelialization through several different statistical analyses. Several proteins from these analyses exhibited significant abundance change between different burn depth or re-epithelialization groups, and can be considered as potential biomarker candidates. Further gene ontology (GO) enrichment analysis of the significant proteins revealed the most significant burn related biological processes (BP) that are altered with burn depth, including homeostasis and oxygen transport. However, for wounds with re-epithelialization times more or less than 21 days, the significant GO annotations were related to enzyme activity. This quantitative proteomics investigation of burn BF may enable objective classification of burn wound severity and assist with clinical decision-making. Data are available via ProteomeXchange with identifier PXD011102.

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“…Collagen types I and III are bioactive substances that are widely present in cartilage, tendons, and other connective tissue; indeed, these fibers constitute the framework of tendons [ 39 , 40 ]. TGF belongs to the TGF superfamily, which regulates cell growth and differentiation and modulates tissue inflammation and repair [ 41 – 43 ].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture

Liu

Jin

et al. 2017

BioMed Research International

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Anterior cruciate ligament injuries are common in humans, though cellular components of the knee have little regenerative or proliferation potential. This study investigated the differentiation of human amnion-derived mesenchymal stem cells (hAMSCs) into human anterior cruciate ligament fibroblasts (hACLFs) in vitro through induction with bFGF and TGF-β1 with coculture systems. Groups A and B comprised hAMSCs at the 3rd passage cultured with and without bFGF and TGF-β1, respectively; Groups C and D consisted of hAMSCs and hACLFs in monolayer coculture with and without bFGF and TGF-β1, respectively; Groups E and F were composed of hAMSCs and hACLFs in Transwell coculture with and without bFGF and TGF-β1, respectively. Cell morphology and proliferation were recorded. Protein expression and relative mRNA expression were evaluated in each group. Cell proliferation was significantly higher in the induced groups than in the noninduced groups. Protein expression increased over time with the highest expression observed in Group E. mRNA levels were significantly higher in Group E than in other groups. This study is the first to demonstrate the use of the Transwell coculture system for this purpose, and hAMSCs were successfully differentiated into hACLFs. Thus, hAMSCs may be a superior choice for hACLF differentiation via Transwell coculture.

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Beta‐catenin causes fibrotic changes in the extracellular matrix via upregulation of collagen I transcription

Cited by 13 publications

References 10 publications

Identification of differentially expressed circular RNAs in keloid and normal skin tissue by high‐throughput sequencing

Identification of differentially expressed circular RNAs in keloid and normal skin tissue by high‐throughput sequencing

Characterization of the Blister Fluid Proteome for Pediatric Burn Classification

Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture

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