Identification of differentially expressed circular RNAs in keloid and normal skin tissue by high‐throughput sequencing

Abstract

Keloid is a kind of pathological skin scar with unclear molecular pathology. Circular RNAs (circRNAs) are involved in the occurrence and development of many diseases; however, their relationship with keloid is not well understood. To investigate the involvement of dysregulated circRNAs in keloid. Thirty-seven keloids and 37 normal skin tissues were collected, and the changes of circRNAs, microRNAs (miRNAs) and mRNAs in 3 keloids and 3 normal samples by high-throughput sequencing were detected first. Based on the circRNA-miRNA-mRNA interaction network construction, gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis combining several signaling pathways associated with keloid formation and progression, the circRNAs required further verification were screened out. The expression levels of the selected circRNAs were verified in 37 keloids and 37 normal skin tissues using quantitative real-time polymerase chain reaction (QPCR). The interaction of candidate circRNA and its predicted binding miRNA was tested by dualluciferase reporter gene experiment. Compared with normal controls, there was an average of 120 and 12 circRNAs, 44 and 63 miRNAs, 656 and 156 mRNAs were upregulated and downregulated, respectively, in keloids. According to the analysis of bioinformation, six circRNAs were picked out. The QPCR validation results of two upregulated circRNAs (hsa_circ_0001320 and circCOL5A1) were consistent with previous sequencing results. The interaction between hsa_circ_0001320 and miR-574-5p was confirmed. This study makes it clear that the abnormal expression of cir-cRNAs may be related to the pathological process of keloid.

Analyses of circRNA and mRNA Profiles in Vogt–Koyanagi–Harada Disease

“…Importantly, based on bioinformatics analysis and validation experiment, we identified that hsa_circ_0001320 and hsa_circ_0001924 may serve as candidate genes for VKH disease. Our results are, by and large, similar to that in keloid reported by Zhang et al ( 32 ). They show that the hsa_circ_0001320 has an aberrantly higher expression in this disease.…”

Circular RNA hsa_circ_0057452 facilitates keloid progression by targeting the microRNA-1225-3p/AF4/FMR2 family member 4 axis

“…Lv et al [ 32 ] found that circCOL5A1 released Epac1 as a competing endogenous RNA via adsorption of miR-7-5p to facilitate pathological hyperplasia of keloids. Zhang et al [ 33 ] revealed that the interaction between hsa_circ_0001320 and miR-574-5p regulates the keloid pathology. In this study, we demonstrated that hsa_circ_0057452 is overexpressed in keloids, which is consistent with previously published circRNA microarray analysis [ 13 ].…”

Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues

“…Nevertheless, the non-self-controlled samples introduced various confounding factors, and long-term culture or in vitro manipulation may alter the epigenetics. Two other articles analyzed the circRNA expression of full-thickness keloid tissues and introduced the impact of epidermal tissues [20,21]. However, none of the four studies have reported the etiology of keloid patients.…”