beta-Adrenergic receptor subtypes in the rat renal cortex. Selective regulation of beta 1-adrenergic receptors by pheochromocytoma.

Snavely, M D; Motulsky, Harvey J.; Moustafa, Esam; Mahan, Lawrence C.; Insel, Paul A.

doi:10.1161/01.res.51.4.504

Cited by 62 publications

(25 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12 Binding of /3-adrenergic receptors to [ 125 I]ICYP was measured by incubating membranes in triplicate with 8-10 concentrations of radioligand (50-600 pmol) at 25° C for 1 hour. Binding was terminated by diluting samples to 10 ml with ice-cold buffer, filtering over Whatman GF/B filters that had been presoaked in 2% polyethylenimine, 13 and washing the filters with 10 ml of buffer.…”

Section: Radioligand Binding Assaymentioning

confidence: 99%

Propranolol treatment externalizes beta-adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia.

Maisel¹,

Motulsky²,

Insel³

1987

Circulation Research

Self Cite

View full text Add to dashboard Cite

Purified sarcolemmal and light vesicle (intracellular) fractions of/3-adrenergic receptors were used to examine the effects of propranolol on receptor translocation in guinea pig heart. Guinea pigs were given propranolol (0.15 mg/kg/hr) via minipumps for 7 days and either killed or made ischemic for 1 hour via a coronary ligature. Propranolol treatment led to an externalization of ^-receptors from light vesicle to sarcolemmal fractions. This externalization increased the number of surface /3-adrenergic receptors that were functional, as assessed by isoproterenol-stimulated adenylate cyclase activity. After chronic propranolol treatment, ischemia did not further alter receptor distribution. These results suggest that externalization of /3-adrenergic receptors from a light vesicle fraction to the sarcolemma contributes to up-regulation of /3-receptors that occur in response to both propranolol treatment and ischemia. Because propranolol-treated animals show blunting in externalization after myocardial ischemia, propranolol treatment and myocardial ischemia appear to access the same pool of intracellular /3-adrenergic receptors. Depletion of this pool of receptors along with receptor blockade may thus contribute to the mechanism by which the drug is efficacious in preventing some adverse effects of ischemia. (Circulation Research 1986;60:108-112)

show abstract

Section: Radioligand Binding Assaymentioning

confidence: 99%

Propranolol treatment externalizes beta-adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia.

Maisel¹,

Motulsky²,

Insel³

1987

Circulation Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The influence of renal sympathetic nerves (which release catecholamines as neurotransmitters) on renal function has been discussed in several reviews (1,6,32). Catecholamines act on target cells by initially binding to cell surface receptors, and we and others (20,(41)(42)(43) have identified a~-, ~2-, B~-, and/~2-adrenergic receptors in membrane preparations of the kidney cortex by radioligand-binding techniques. However, the sites of action of adrenergic agents along the nephron have not been clearly defined.…”

mentioning

confidence: 99%

alpha 1- and beta 2-adrenergic receptor expression in the Madin-Darby canine kidney epithelial cell line.

Meier¹,

Snavely²,

Brown³

et al. 1983

The Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/ collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selective adrenergic agonists and antagonists, that MDCK cells have both at-and ~2-adrenergic receptors. To determine whether these receptor types were expressed by the same cell, we developed a number of clonal MDCK cell lines. The clonal lines had stable but unique morphologies reflecting heterogeneity in the parent cell line. Some clones expressed only/~2-adrenergic receptors and were nonmotile, whereas others expressed both ~t-and /~2-receptors and demonstrated motility on the culture substrate at low cell densities. In one clone, a-and/~-receptor expression was stable for more than 50 passages. Catecholamine agonists increased phosphatidylinositol turnover by activating a-adrenergic receptors and cellular cyclic adenosine monophosphate accumulation by activating/3-adrenergic receptors. Guanine nucleotide decreased the affinity of isoproterenol for the ~2-receptor but did not alter the affinity of epinephrine for the c~l-receptor. These results show that c~l-and/32-receptors can be expressed by a single renal tubular cell and that the two receptors behave as distinct entities in terms of cellular response and receptor regulation. Heterogeneity of adrenergic receptor expression in MDCK clones may reflect properties of different types of renal tubule cells.Catecholamines regulate a variety of cellular functions in the mammalian kidney, including tubule water and ion reabsorption, renin release, renal hemodynamics, and gluconeogenesis (20,35). The influence of renal sympathetic nerves (which release catecholamines as neurotransmitters) on renal function has been discussed in several reviews (1,6,32). Catecholamines act on target cells by initially binding to cell surface receptors, and we and others (20,(41)(42)(43) have identified a~-, ~2-, B~-, and/~2-adrenergic receptors in membrane preparations of the kidney cortex by radioligand-binding techniques. However, the sites of action of adrenergic agents along the nephron have not been clearly defined. Determination of the location of catecholamine action within the kidney is particularly important to distinguish indirect adrenergic effects (resulting from changes in vascular tone) from direct effects on epithelial transport and metabolism. The kidney consists of segments that are anatomically and functionally distinguishable, yet each segment may contain several morphologically distinct cell types (8). To understand the regulation of renal function by catecholamines and sympathetic nerves, it therefore is important to study homogeneous populations of renal cells. Such populations may be found in the established renal cell lines, which have provided valuable information r...

show abstract

“…Though sensitivity to catecholamines could not be confirmed in the present case because of lack of loading test owing to cerebral hemorrhage, it was speculated that the sensitivity of catecholamines receptors might be extremely high. It has been reported that in pheochromocytoma, a-receptors and (3-receptors in the cardiovascular system generally may be downregulated to excessive catecholamines ( 16,17), and the enhanced sensitivity in this case was considered quite interesting. Astudy on the sensitivity of this case to catecholamines is scheduled after obtaining consent of the patient himself.…”

Section: Discussionmentioning

confidence: 74%

Pheochromocytoma of the Urinary Bladder Revealed with Cerebral Hemorrhage.

et al. 2001

View full text Add to dashboard Cite

The case was a 51-year-old man, who has been undergoing treatment with oral medication for hypertension for three years. The patient was admitted to the author's clinic for hemorrhage in the left putamen. He was diagnosed as having primary pheochromocytoma of the bladder from such symptoms as paroxysmal blood pressure elevation after urination, mild increase in catecholamine levels before and after urination, and from the results of 131I-MIBGscintigraphy, and cystoscopy, and underwent excision of the bladder tumor. Uponendocrinological examination, only mild increases in catecholamine levels were found. Therefore, constant monitoring of blood pressure and 131I-MIBG scintigraphy were useful for a definitive diagnose. (Internal Medicine 40: 638-642, 2001)

show abstract

beta-Adrenergic receptor subtypes in the rat renal cortex. Selective regulation of beta 1-adrenergic receptors by pheochromocytoma.

Cited by 62 publications

References 27 publications

Propranolol treatment externalizes beta-adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia.

Propranolol treatment externalizes beta-adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia.

alpha 1- and beta 2-adrenergic receptor expression in the Madin-Darby canine kidney epithelial cell line.

Pheochromocytoma of the Urinary Bladder Revealed with Cerebral Hemorrhage.

Contact Info

Product

Resources

About