Beta 1, Beta 2 and Beta 3 Adrenergic Receptor Gene Polymorphisms in a Southeastern European Population

Abstract

Genetic polymorphisms in β1-, β2- and β3-adrenergic receptors (β-ARs) have been associated with chronic non-communicable disorders, such as cardiovascular diseases, asthma, chronic obstructive pulmonary disease (COPD) and obesity, as well as β-agonists and antagonists response and toxicity. The purpose of this study was to determine the frequency distribution of ADRB1 genetic variants Ser49Gly and Arg389Gly, ADRB2 variants Gly16Arg and Gln27Glu, ADRB3 variant Trp64Arg in a Southeastern European Caucasian (SEC) population sample and to establish a comparison with existing data from other human populations. A sample of 431 men and 590 women volunteered to participate in this genotyping analysis after anonymization and de-identification. Real Time PCR (Melting Curve Analysis) followed DNA extraction from buccal swabs and statistical analysis of the results was performed. The allele frequencies in the SEC population were Ser49 (90.3%), Arg389 (69.49%), Gly16 (61.61%), Gln27 (65.72%), and Trp64 (94.52%), while a Hardy-Weinberg Equilibrium (HWE) was detected in the population studied. Comparisons for the Ser49Gly, Gln27Glu, and Trp64Arg allele distributions demonstrated significant differences between SEC and the European group. European subgroups comparisons showed that allele distributions were similar for four of the five SNPs between SEC and Southwestern European Caucasians (SWC), while they were quite distinct from the Northwestern European Caucasians (NWC). These data underline the importance of interethnic variability of β-ARs genetic polymorphisms.

Impact of vitamin�D receptor gene polymorphisms on vitiligo susceptibility and clinical features in�a�Southeastern European Caucasian population

“…This study presents some limitations to consider. Apart from the relatively small sample size (110 cases), our analyses included data only from SEC subjects, thus making the results applicable only to this ethnotic group, especially considering that genetic variability differs within European populations ( 32 - 34 ). Moreover, investigations on other common VDR SNPs, such as the Apa I, as well as haplotype analysis was not conducted, thus limiting the acquisition of further genetic information.…”

Obesity ‑ a risk factor for increased COVID‑19 prevalence, severity and lethality (Review)

“…Furthermore, the prolonged iFn responses during persistent chronic inflammation and obesogenesis comprise reciprocal causality between virus susceptibility and obesity (80). additional epigenetic signatures in obesity are likewise altered including methylation and/or histone acetylation levels in genes involved in specific and general metabolic processes, altering thus, the metabolic phenotype of the offspring (81)(82)(83). although no specific therapy exists to block the effects of these factors, recognizing the high risk and anticipating inflammation-associated complications of adipokine release is an important part of optimal patient management.…”

Association of RAGE gene multiple variants with the risk for COPD and asthma in northern Han Chinese

“…Recently, several genes have been documented to be simultaneously associated with the risk for COPD and asthma [11–13], indicating that COPD and asthma might share a common genetic origin that is possibly involved in the development of the lungs [14–17]. Experimental studies supported the strategies of inhibiting the receptor for advanced glycation end products (RAGE) in lung injury [18], and the contribution of RAGE to chronic inflammation, suggesting that RAGE may be a therapeutic target for lung-related diseases [19].…”