Bestimmung von Thiolverbindungen durch selektive Fluorescenzderivatisierung und HPLC

Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

“…The most frequently used method for total plasma aminothiol measurement is RP-HPLC with fluorescent detection after derivatization. Commonly used fluorogenic reagents include monobromobimane (mBrB) [8,9], 9-acetoxy-2-(4-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)phenyl)-3-oxo-3H-naphtho[2,1-b]pyran (ThioGlo) [10], ammonium 7-fluorobenzo-2-oxa-1,3 diazoloe-4-sulfonate (SBD-F) [11,12], 7-fluoro-2,1,3-benzoxadiazole-4-sulfonamide (ABD-F) [13,14], N-(1-pyrenil) maleimide (NPM) [15], dansylaziridine [16]. These reagents are not totally satisfactory.…”

New fluorogenic reagent having halogenobenzofurazan structure for thiols: 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole

“…A few types of reagents, dansylaziridine, bimane, and maleimide, have been used for the purpose. Dansylaziridine is fluorescent itself and gives a large interfering peak (1). Bimane is not selective for thiol because it also reacts with alcohol, phenol, and/or amino groups (2).…”

Spectrofluorometric determination of common epoxides with sodium sulfide and o-phthalaldehyde and taurine reagents

“…Compared with other fluorogenic reagents for thiols such as halogenated benzofurazan derivatives (12,13), dansylazirizine (14), and N-substituted maleimide derivatives (15,16), the OPA reaction seemed appropriate for our purpose because this reaction was completed immediately and fortunately the fluorescence intensity resulting from sodium sulfide was very weak (7).…”