Bestimmung der Reihenfolge der Aminosäuren am Carboxylende des Tabakmosaikvirus durch Hydrazinspaltung

Braunitzer, Gerhard

doi:10.1002/cber.19550881235

Cited by 93 publications

(6 citation statements)

References 11 publications

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“…The configuration of the amino acids was determined either enzymatically (216,271,336) or by measuring the optical rotatory dispersion of the 2, 4-dinitrophenyl (DNP) derivatives (50,176). The Cand N-terminal amino acids of the peptidoglycan and of peptides were determined by hydrazinolysis (47,176) and by dinitrophenylation (114,310).…”

Section: Determination Of the Amino Acidmentioning

confidence: 99%

Peptidoglycan types of bacterial cell walls and their taxonomic implications

Schleifer¹,

Kandler²

1972

Bacteriol Rev

2,331

590

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Section: Determination Of the Amino Acidmentioning

confidence: 99%

Peptidoglycan types of bacterial cell walls and their taxonomic implications

Schleifer¹,

Kandler²

1972

Bacteriol Rev

2,331

590

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“…However, the known instability of the hydrazides of glycine and serine [24,25] as well as a kinetic study (Table 2) favoured the opinion that their occurrence in C-terminal position has to be considered as negligible.…”

Section: After Chymotrypsin Cleavage Of the C-terminalmentioning

confidence: 99%

Structural and Immunogenic Properties of a Major Antigenic Determinant in Neutral Salt‐Extracted Rat‐Skin Collagen

Stoltz

Timpl

Kühn

et al. 1973

European Journal of Biochemistry

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The major antigenic determinant of neutral salt-extracted rat-skin collagen is located in the C-terminal CNBr peptide al-CBGb of the d-chain. This peptide is shortened by 20 amino acid residues from its C-terminal nonhelical end if compared with al-CB6 obtained from collagen mi-chains extracted under denaturating conditions. As reported earlier, degradation by tissue proteases during extraction was assumed as the main reason for the observed reduction in size.The C-terminal sequence of al-CB6b was determined as Tyr-Asp and was found to be essential for antigenic activity. Proportional loss of binding to antibodies with decrease in size was observed for appropriate proteolytic fragments derived from a1 -CB6b. Since this peptide extends only five amino acid residues beyond the helical collagen sequence, an important contribution of the conformational structure for this antigenic determinant is suggested. Decrease in ability to induce high antibody titers to ai-CB6b was already observed a t a higher structural level, e.g. after denaturation of triple-helical collagen to random-coiled a-chains. The same result could be obtained if adjuvant was omitted in the priming injection.It hag been demonstrated recently that collagen can be used as a good model to correlate antigenic specificity with discrete amino acid sequences. The sites so far elucidated as to their structure [i-31 are located in the terminal, nonhelical sequences of the a-chains which represents a length of about 401, of the total amino acid sequence of the molecule. These nonhelical areas are obviously not involved in the triple-helical collagen structure which explains their sensitivity to proteolytic digestion [4] a finding correlating well with earlier immunologic experience [5 -91. In acid-soluble calf-skin collagen the moat important antigenic determinant could be assigned to the C-terminal cyanogen bromide (CNBr) peptide al-CB6 of the al-chain [7] and was later located in a hexapeptide sequence [3] of the nonhelical region involving 25 amino acid residues of al-CB6 [iO,il]. A limited number of the antisera investigated contained additional antibodies which reacted with an antigenic determinant on a shorter CNBr peptide al-CB6b and apparently required the free cctrboxyl group of a leucine residue located in the region of the original antigenic determinant.Since collagen a-chains extracted under denaturating conditions did not reveal this new antigenic determinant, its creation by tissue proteases being active under the conditions of collagen extraction with neutral salts or acidic buffers was assumed [3].Artificial shortening of the manner described was suspected to be even more prominent in the al-chain of neutral salt-extracted rat-skin collagen [12]. I n spite of this the predominant antigenic determinant of this kind of collagen has been demonstrated on the same C-terminal CNBr peptide [8,13] as in calf collagen. A location close to the C-terminal end was suggested by the sensitivity to further degradation by pronase but not by pepsin [8,9]. The...

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“…Hydroazinolysis. Anhydrous hydrazine was prepared by Braunitzer's (1955) method and the course of the experiments followed from his data. DNA (30 mg. each from 0 07 and 0-14M-benzoate preparations) was used with 0 3 ml.…”

Section: Methodsmentioning

confidence: 99%

Preparation of some deoxyribonucleic acid–protein complexes from rat-liver homogenates

Kirby

1958

Biochemical Journal

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Bestimmung der Reihenfolge der Aminosäuren am Carboxylende des Tabakmosaikvirus durch Hydrazinspaltung

Cited by 93 publications

References 11 publications

Peptidoglycan types of bacterial cell walls and their taxonomic implications

Peptidoglycan types of bacterial cell walls and their taxonomic implications

Structural and Immunogenic Properties of a Major Antigenic Determinant in Neutral Salt‐Extracted Rat‐Skin Collagen

Preparation of some deoxyribonucleic acid–protein complexes from rat-liver homogenates

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