Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin

Musumeci, Rosario; Speciale, A; Costanzo, R.; Annino, A.; Ragusa, S.; Rapisarda, Antonio; Pappalardo, Marco; Iauk, L.

doi:10.1016/s0924-8579(03)00085-2

Cited by 105 publications

(58 citation statements)

References 8 publications

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“…As could be expected [8,94], the growth rate in the absence and at a low concentration of erythromycin (0.0625 g mL −1 ) (Figure 4(b)) was greatest in the aerobic, naïve cultures and lowest for the highly resistant strain-the latter effect being due to a "fitness cost" as entailed by most antimicrobial resistance mechanisms [88]. The intrinsic growth inhibition of anaerobic, naïve cultures of S. aureus indicates that pyp, provided it equals php in its function as an EPI [51,52], acts against a metabolically regulated MDR pump with broader, compound nonspecific functions unrelated to antimicrobials. It can be concluded that this hypothetical MDR efflux pump is one other than NorA, as there is no strong indication of erythromycin being a NorA substrate [29,60,82,83] and as we observed no significant effect of pyp against Ent.…”

Section: Discussionmentioning

confidence: 99%

“…It is not an uncommon feature for an EPI from natural sources to possess a direct antibacterial effect [26,35]. For example, the aerobic MIC of php was found to vary between 4 g mL −1 (S. epidermidis) and 500 g mL −1 (S. aureus ATCC 29213) [52], and in another study the death percentage of S. aureus ATCC 26923 in 20 g php mL −1 was 32% [114]. It is conceivable that, as discussed above for S. aureus, pyp exerted its intrinsic effect on the manure microbiota through inhibition of a MDR efflux pump, such as LmrS, MdeA, and possibly NorA, as these and their homologues occur in B. subtilis, a major component of MicroSource S, and are widespread among other low mol percentage guanine-cytosine Gram-positive (phylum Firmicutes) bacteria [27,81,97,100,[115][116][117], the predominant culturable anaerobic microorganisms from swine manure [73,118,119].…”

Section: Discussionmentioning

confidence: 99%

“…The EPI activity of php was first deduced from its berberine-and norfloxacin-potentiating, antimicrobial effect against S. aureus bearing the NorA MDR pump [51]. Later on, this effect was extended to ciprofloxacin and other strains of S. aureus, S. epidermidis, Escherichia (E.) coli, and Pseudomonas aeruginosa [52]. An EPI activity of pyp has not been reported at the time of writing.…”

Section: International Journal Of Antibioticsmentioning

confidence: 99%

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Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbideaand Pyropheophorbideaon Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

Kraatz

Whitehead

Cotta

et al. 2014

International Journal of Antibiotics

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Natural plant compounds, such as the chlorophyll a catabolites pheophorbide a (php) and pyropheophorbide a (pyp), are potentially active in the gastrointestinal tracts and manure of livestock as antimicrobial resistance-modifying agents through inhibition of bacterial efflux pumps. To investigate whether php, a known efflux pump inhibitor, and pyp influence bacterial resistance, we determined their long-term effects on the MICs of erythromycin for reference strains of clinically relevant indicator bacteria with macrolide or multidrug resistance efflux pumps. Pyp reduced the final MIC endpoint for Staphylococcus (S.) aureus and Escherichia (E.) coli by up to 1536 and 1024 g erythromycin mL −1 or 1.4-and 1.2-fold, respectively. Estimation of growth parameters of S. aureus revealed that pyp exerted an intrinsic inhibitory effect under anaerobic conditions and was synergistically active, thereby potentiating the effect of erythromycin and partially reversing high-level erythromycin resistance. Anaerobe colony counts of total and erythromycin-resistant bacteria from stored swine manure samples tended to be lower in the presence of pyp. Tylosin, php, and pyp were not detectable by HPLC in the manure or medium. This is the first study showing that pyp affects growth and the level of sensitivity to erythromycin of S. aureus, E. coli, and anaerobic manure bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: International Journal Of Antibioticsmentioning

confidence: 99%

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Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbideaand Pyropheophorbideaon Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

Kraatz

Whitehead

Cotta

et al. 2014

International Journal of Antibiotics

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“…Berberine is an alkaloid available from the medicinal plant Coptis chinensis that is widely used clinically in the treatment of gastrointestinal infections, diabetes, hypertension, and hypercholesterolemia. There are numeros drug-drug interaction studies related to berberine with Chinese medicine or drugs such as Jiao-Tai-Wan, verapamil, flavonoids of radix Scutellariae, digoxin, simvastatin, ciprofloxacin, fluconazole, doxorubicin, pravastatin, rhein, cyclosporine A, ketoconazole, losartan, probenecid, cloxacillin, midazolam, quinidine and atorvastatin [16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Combination of simvastatin with berberine improves the lipid-lowering efficacy [20].…”

Section: Introductionmentioning

confidence: 99%

In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies

et al. 2016

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Background:We assumed that the pharmacokinetics of lovastatin could be changed by the induction effect of berberine. Methods: An UPLC-MS/MS method was developed and validated for the pharmacokinetics tudy of lovastatin to investigate the in vivo drug-drug interactions between lovastatin and berberine. SD male rats were random divided into lovastatin group and berberine induced prior to lovastatin group for the in vivo pharmacokinetic studies. Meanwhile HepG2 cells were induced by berberine for three days to study the metabolism of lovastatin. Results: The AUC (p < 0.01) and C max (p < 0.01) could be significantly decreased in the berberine-induced group in vivo, and the metabolic activity of HepG2 cell ccould be increased by berberine induction in vitro. The metabolism parameters of lovastatin such as CL, V max and K m were increased after the induction of berberine. From the pharmacokinetic study of lovastatin induced with berberine, we obtained pharmacokinetic parameters which are compliance with the metabolic parameters of lovastatin in HepG2 cells with berberine induction in vitro. Conclusions: From the in vivo pharmacokinetics study and the HepG2 cell metabolism study in vitro, berberine could be an inducer for the metabolism of lovastatin according to our previous research on berberine induction effects on HepG2 cells, which may be relevant to the fact that berberine possesses induction effects through the CYP 450 3A4 enzyme.

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“…It is found to have a varied pharmacology including antidiarrheal, antiarrhythmic activities, and antimicrobial activity. [32][33][34][35] In traditional medicine, the extracts of various Berberidaceae (Berberis aquifolium, Berberis vulgaris, and Berberis aristata) are used to treat rheumatic complaints and other types of chronic inflammations. Furthermore, berberine displayed a significant antimicrobial activity against a variety of organisms including bacteria, viruses, and fungi, and showed antimicrobial activity against all tested strains of MRSA.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic delivery system using nano‐hydroxyapatite/chitosan bone cement consisting of berberine

Zou

Zhang

et al. 2008

J Biomedical Materials Res

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Different concentrations of berberine were mixed with nano-hydroxyapatite/chitosan (n-HA/CS) bone cement to generate an antibiotic drug delivery system for treatment of bone defects. Properties of the system such as setting time, compressive strength, surface morphology, phase compositions, drug release profiles and antimicrobial activity were also characterized. It was shown that the setting time of the cement ranged from 17.03 +/- 0.50 min to 28.47 +/- 0.96 min and the compressive strength changed from 184.00 +/- 7.94 MPa to 120.33 +/- 9.02 MPa with the increase of berberine. The XRD, IR, and SEM analyses suggested that berberine powders were stable in the bone cement in simulated body fluid (SBF). In vitro release of berberine from the bioactive bone cement pellets in SBF could last more than 4 weeks. The release profiles of 1.0 wt % berberine loaded bone cement followed the Higuchi equation at the infusion stage. The drug loaded pellets can inhibit bacterial growth (Staphylococcus aureus) at the standardized berberine minimum inhibitory concentration of 0.02 mg/mL during berberine release from 1 to 28 days. The n-HA/CS bone cement only with 1.0 wt % berberine proved to be an efficient antibiotic drug delivery system.

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Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin

Cited by 105 publications

References 8 publications

Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbideaand Pyropheophorbideaon Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbideaand Pyropheophorbideaon Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies

Antibiotic delivery system using nano‐hydroxyapatite/chitosan bone cement consisting of berberine

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