Berberine: Complex with DNA

Krey, Anne K.; Hahn, Fred E.

doi:10.1126/science.166.3906.755

Cited by 103 publications

(41 citation statements)

References 15 publications

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“…Herbal remedies, such as BBR act via interactions with DNA and RNA, cell cycle arrest, and anti-inflammatory and anti-angiogenesis activities (24). The findings of the current study indicated that BBR may be more active in the inhibition of tumor cell proliferation, although BBR shows minor cytotoxicity against normal cells.…”

Section: Discussionmentioning

confidence: 55%

Antiproliferative effect of berberine on canine mammary gland cancer cell culture

Sefidabi¹,

Mortazavi²,

Hosseini³

2016

Biomedical Reports

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Abstract. Canine mammary gland tumors are the most frequent cause of cancer in female dogs. Numerous studies using cancer cell lines and clinical trials have indicated that various natural products and antioxidants reduce or possibly prevent the development of cancer. Berberine (BBR), the most important alkaloid in the Berberidaceae, which exerts a wide range of pharmacological and biochemical effects, has drawn much attention due to its particularly high antitumor activity in vitro and in animal studies. The aim of the present study was to investigate the antiproliferative effect of BBR against a canine mammary gland carcinoma cell line (CF41.Mg) in vitro. CF41.Mg cells were cultured in RPMI-1640 medium containing 10% heat inactived fetal bovine serum (FBS) and 100 mg/ml peniciline-streptomycin. Subsequently the cells were treated with different concentrations of BBR chloride (10, 25, 50, 100 and 200 µM) at a density of 12,000 cells/well in 96-well plates. Following treatment, the MTT assay was used to detect cell viability after 24-, 48-and 72-h incubations at 37˚C with 5% CO 2 . The results indicated that BBR inhibited proliferation of canine mammary gland carcinoma cells, as treatment with 100 µM BBR for 24 h resulted in a significant decrease in cell viability (P<0.005). As the present study demonstrated that BBR (10-200 µM) induced cancer cell death, it is proposed that BBR may serve as a candidate agent against canine mammary tumor cells via its antiproliferative activity.

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Section: Discussionmentioning

confidence: 55%

Antiproliferative effect of berberine on canine mammary gland cancer cell culture

Sefidabi¹,

Mortazavi²,

Hosseini³

2016

Biomedical Reports

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“…The drug thus appears to have a broad antimicrobial activity (2) and seems to be relatively nontoxic to humans (9,18), even though it and its derivatives intercalate themselves into DNA and phospholipid bilayers (5,13) and inhibit enzymes such as alcohol dehydrogenase and xanthine oxidase (22). These latter activities probably account for the ability of berberine sulfate to inhibit reverse transcriptase activity and thereby to inhibit the replication of certain retroviruses (22).…”

mentioning

confidence: 99%

Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane

Sun

Courtney

Beachey

1988

Antimicrob Agents Chemother

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Berberine sulfate is an alkaloid extracted from the roots and bark of various plants and possesses antibacterial, antifungal, and antiprotozoal activities. Most studies have focused on the bacteriostatic or bactericidal activities of this compound. In this study, we report that berberine sulfate is bacteriostatic for streptococci and that sub-MICs of berberine blocked the adherence of streptococci to host cells, immobilized fibronectin, and hexadecane. Concentrations of berberine below its MIC caused an eightfold increase in release of lipoteichoic acid from the streptococci. Higher concentrations of berberine directly interfered with the adherence of streptococci to host cells either by preventing the complexing of lipoteichoic acid with fibronectin or by dissolution of such complexes once they were formed. Thus, berberine sulfate interferes with the adherence of group A streptococci by two distinct mechanisms: one by releasing the adhesin lipoteichoic acid from the streptococcal cell surface and another by directly preventing or dissolving lipoteichoic acid-fibronectin complexes.Berberine sulfate is an extract of plants that has been used either as a crude extract or in pure form to treat trachoma (19), diarrhea (9, 14, 18, 20), amoebiasis (12, 24), and pharyngeal pyogenic infections (25). The drug thus appears to have a broad antimicrobial activity (2) and seems to be relatively nontoxic to humans (9, 18), even though it and its derivatives intercalate themselves into DNA and phospholipid bilayers (5, 13) and inhibit enzymes such as alcohol dehydrogenase and xanthine oxidase (22). These latter activities probably account for the ability of berberine sulfate to inhibit reverse transcriptase activity and thereby to inhibit the replication of certain retroviruses (22).The efficacy of berberine sulfate in the treatment of certain bacterial diseases appears to be primarily dependent upon its direct antibacterial effects. In some cases, however, berberine appears to be efficacious even though high concentrations of the drug have no effect on bacterial growth. The therapeutic effect of berberine sulfate against enterotoxigenic Escherichia coli was suggested to be due to its antisecretory effects on the host rather than any effect on the invading organisms (18,20).In most studies of the anti-infectious activities of berberine sulfate, the focus has been on its antimicrobial effects; little or no attention has been paid to the effects of berberine on the adherence of bacteria to host cells. Since adherence of an organism to mucosal surfaces usually precedes invasion, some of the beneficial properties of berberine could be due to interference with the adherence process. The present study was undertaken to determine the effects of berberine sulfate on the adhesive properties of Streptococcus pyogenes. These microorganisms adhere to epithelial cells (3, 4), fibronectin (6, 23), and hexadecane (7, 17); in each case, lipoteichoic acid (LTA) has been found to be the major streptococcal surface molecule involved in adherence...

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“…Some studies suggest that berberine may either directly bind duplex DNA by intercalation (27)(28)(29)(30) or inflict DNA damage indirectly by its interaction with topoisomerases I and II (28,(31)(32)(33). Other investigators also showed that berberine at 150 mg/ml was able to induce the rate of sister chromatid exchanges, which reflects homologous recombination repair, by several fold (34).…”

Section: Discussionmentioning

confidence: 99%

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

et al. 2009

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Abstract. The cytotoxicity of berberine on C6 rat glioma cells indicated that berberine induced morphological changes and caused cell death through G2/M arrest and apoptosis. While undergoing apoptosis, there was a remarkable accumulation of G2/M cells with the upregulatoin of Wee1 but it also inhibited cyclin B, CDK1 and Cdc25c that led to G2/M arrest. Along with cytotoxicity in C6 cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9, -3 and -8 and DNA fragmentation were induced. Berberine increased the levels of GADD153 and GRP 78 in C6 cells based on the examination of Western blotting and this is a major hallmark of endoplasmic reticulum (ER) stress. We also found that berberine promoted the production of reactive oxygen species and Ca 2+ in C6 cells. Western blotting assay also showed that berberine inhibited the levels of anti-apoptotic protein Bcl-2 but increased the levels of pro-apoptotic protein Bax before leading to a decrease in the levels of mitochondrial membrane potential (Δae m ) followed by cytochrome c release that caused the activations of capase-9 and -3 for apoptotic occurrence. The caspase-8, -9 and -3 were activated by berberine in C6 cells based on the substrate solution (PhiPhiLux-G 1 D 1 , CaspaLux 8-L 1 D 2 , CaspaLux 9-M 1 D 2 for caspase-3, -8 and -9, respectively) and analyzed by flow cytometer and each inhibitor of caspase-8, -9 and -3 led to increase the percentage of viable C6 cells after exposure to berberine. This finding was also confirmed by Western blot assay which showed that berberine promoted the active form of caspase-8, -9 and -3. These results demonstrate that the cytotoxicity of berberine in C6 rat glioma cells is attributable to apoptosis mainly through induced G2/M-arrested cells, in an ER-dependent manner, via a mitochondria-dependent caspase pathway regulated by Bax and Bcl-2.

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Berberine: Complex with DNA

Cited by 103 publications

References 15 publications

Antiproliferative effect of berberine on canine mammary gland cancer cell culture

Antiproliferative effect of berberine on canine mammary gland cancer cell culture

Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

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